Skip to main content

Advertisement

SpringerLink
Log in

A longitudinal survey of gastrointestinal parasites of the black-eared opossum Didelphis aurita at an urban-sylvatic interface area in Southeast Brazil: a morphological and ecological characterization based on helminth eggs

  • Helminthology - Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Parasites are important organisms in ecosystem dynamics and take part in the regulation and structure of host populations. The prevalence, abundance, and distribution of parasites in their host species may be affected by biotic and abiotic factors. Most studies of helminths of Brazilian marsupials are taxonomic descriptions or re-descriptions and records of occurrence. The use of noninvasive techniques for studying parasitic worms of vertebrate hosts is more common in large or threatened species. The aims of this study were to describe and identify the helminth morphotypes and to analyse the parasitological parameters of gastrointestinal helminth eggs obtained in faecal samples of marsupial Didelphis aurita at the Fiocruz Atlantic Forest Biological Station and surroundings, municipality of Rio de Janeiro, southeast Brazil. The common opossums were sampled during a capture-mark-recapture study. Faecal samples collected from each animal were analysed for helminth egg diagnosis using the methods of flotation in sugar solution and sedimentation. Eggs were compared with samples obtained from the uterus of adult worms obtained from a previous study carried out in the same area. Eleven helminth egg morphotypes were found. Among them, seven were identified at the species level. The highest values of parasitic load and prevalence were observed for the families Viannaiidae and Trichuridae. A significant relationship was found between the number of morphotypes and host body size in females. The influence of parasite load on host condition factor or body size was not observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

Code availability

Not applicable.

References

  • Adnet FAO, Anjos DHS, Menezes-Oliveira A, Lanfredi RM (2009) Further description of Cruzia tentaculata (Rudolphi, 1819) Travassos, 1917 (Nematoda: Cruzidae) by light and scanning electron microscopy. Parasitol Res 104:1207–1211. https://doi.org/10.1007/s00436-008-1316-6

    Article  CAS  PubMed  Google Scholar 

  • Anderson RC (2000) Nematode parasites of vertebrates: their development and transmission, 2nd end. Biddles Ltd, Guildford and King’s Lynn, London

    Google Scholar 

  • Antunes GM (2005) Diversidade e potencial zoonótico de parasitos de Didelphis albivrentris Lund, 1841 (Marsupialia: Didelphidae). Thesis, Universidade Federal do Rio Grande do Sul

  • Aragón-Pech RA, Ruiz-Piña HA, Rodríguez-Vivas RI, Cuxim-Koyoc AD, Reyes-Novelo E (2018) Prevalence, abundance and intensity of eggs and oocysts of gastrointestinal parasites in the opossum Didelphis virginiana Kerr, 1792 in Yucatan, Mexico. Helminthol 55:119–126. https://doi.org/10.2478/helm-2018-0008

    Article  Google Scholar 

  • Ayoade JO (1986) Introdução à climatologia para os trópicos. Difel, São Paulo

    Google Scholar 

  • Behnke JM, Lewis JW, Mohd Zain SN, Gilbert FS (1999) Helminth infections in Apodemus sylvaticus in southern England: interactive effects of host-age, sex and year on prevalence and abundance of infections. J Helminthol 73:31–44. https://doi.org/10.1017/S0022149X99000049

    Article  CAS  PubMed  Google Scholar 

  • Bezerra-Santos MA, Fontes CS, Nogueira BCF, Yamatogi RS, Ramos RAN, Galhardo JA, Furtado LFV, Rabelo EML, Araújo JV, Campos AK (2020a) Gastrointestinal parasites in the opossum Didelphis aurita: are they a potential threat to human health? J Parasit Dis 44:355–363. https://doi.org/10.1007/s12639-020-01205-9

    Article  PubMed  PubMed Central  Google Scholar 

  • Bezerra-Santos MA, Furtado LFV, Rabelo EML, Nogueira BCF, Yamatogi RS, Campos AK (2020b) High prevalence of Ancylostoma caninum infection in black-eared opossums (Didelphis aurita) in an urban environment. Parasitol Res 119:2343–2346. https://doi.org/10.1007/s00436-020-06708-1

    Article  PubMed  Google Scholar 

  • Bezerra-Santos MA, Ramos RAN, Campos AK, Dantas-Torres F, Otranto D (2021) Didelphis spp. opossums and their parasites in the Americas: a one health perspective. Parasitol Res. https://doi.org/10.1007/s00436-021-07072-4

    Article  PubMed  PubMed Central  Google Scholar 

  • Bowman DD (1999) Georgis’ parasitology for veterinarians, 7th edn. WB Saunders Company, Philadelphia

    Google Scholar 

  • Bush AO, Lafferty KD, Lotz JM, Shostak AW (1997) Parasitology meets ecology on its own terms: Margolis et al Revisited*. J Parasitol 83:575–583. https://doi.org/10.2307/3284227

    Article  CAS  PubMed  Google Scholar 

  • Byles B, Catzeflis F, Scheibel RP, Agustín JFA (2013) Gastrointestinal helminths of two species of mouse opossums (Marmosa demerarae and Marmosa murina) from French Guiana. Comp Parasitol 80:210–216

    Article  Google Scholar 

  • Caballero E, Barroeta LF, Grocott RG (1956) Helmintos de la República de Panamá. Rev Biol Trop 4:161–177

    Google Scholar 

  • Cáceres NC, Monteiro-Filho E (2001) Food habits, home range and activity of Didelphis aurita (Mammalia, Marsupialia) in a forest fragment of Southern Brazil. Stud Neo Fauna Environ 36:85–92

    Article  Google Scholar 

  • Castro RGBM, Costa-Neto SF, Maldonado AJ, Gentile R (2017) Ecological aspects of nematode parasites of Didelphis aurita (Didelphimorphia, Didelphidae) in urban-sylvatic habitats in Rio de Janeiro, Brazil. Oecol Aust 1:54–61. https://doi.org/10.4257/oeco.2017.2101.06

    Article  Google Scholar 

  • Chagas-Moutinho VA, Oliveira-Menezes A, Cárdenas MQ, Lanfredi RM (2007) Further description of Aspidodera raillieti (Nematoda: Aspidoderidae) from Didelphis marsupialis (Mammalia: Didelphidae) by light and scanning electron microscopy. Parasitol Res 101:1331–1336. https://doi.org/10.1007/s00436-007-0641-5

    Article  CAS  PubMed  Google Scholar 

  • Chagas-Moutinho VA, Sant’Anna V, Oliveira-Menezes A, De Souza W (2014) New Aspidoderidae species parasite of Didelphis aurita (Mammalia: Didelphidae): a light and scanning eléctron microscopy approach. Acta Trop 130:162–166. https://doi.org/10.1016/j.actatropica.2013.10.005

    Article  CAS  PubMed  Google Scholar 

  • Cirino BS, Costa Neto SF, Maldonado Júnior A, Gentile R (2020) First study on the helminth community structure of the neotropical marsupial Metachirus myosuros (Didelphimorphia, Didelphidae). Rev Bras Parasitol Vet 29:1–13. https://doi.org/10.1590/S1984-29612020064

    Article  Google Scholar 

  • Combes C (2001) Parasitism: the ecology and evolution of intimate interactions, 1st end. University of Chicago Press, Chicago

    Google Scholar 

  • Costa-Neto SF, Simões RO, Mota EM, Vilela RV, Torres EJL, Barbosa HS, Gentile R, Maldonado A Jr (2016) Lungworm Heterostrongylus heterostrongylus Travassos, 1925 from the black-eared opossum in South America: morphologic, histopathological and phylogenetic aspects. Vet Parasitol 228:144–152. https://doi.org/10.1016/j.vetpar.2016.08.018

    Article  PubMed  Google Scholar 

  • Costa-Neto SF, Cardoso TS, Boullosa RG, Maldonado A Jr, Gentile R (2019) Metacommunity structure of the helminths of the black-eared opossum Didelphis aurita in peri-urban, sylvatic and rural environments in south-eastern Brazil. J Helminthol 93:720–731. https://doi.org/10.1017/S0022149X18000780

    Article  CAS  PubMed  Google Scholar 

  • Durette-Desset MC (1968) Helmintes de marsupiaux américains, Trichostrongyloidea. Bull Soc Zoo Fr 93:581–594

    Google Scholar 

  • Faria MB, Lanes RO, Bonvicino CR (2019) Marsupiais do Brasil: guia de identificação com base em caracteres morfológicos externos e cranianos, 1st edn. Amélie Editorial, São Caetano do Sul

    Google Scholar 

  • Freitas JFT, Lent H (1936) Estudo sobre o gênero Globocephalus Molin, 1861 (Nematoda: Strongyloidea). Mem Inst Oswaldo Cruz 31:69–83

    Article  Google Scholar 

  • Freitas JFT, Kohn A, Ibáñez NH (1967) Sobre espécies de Brachylaemus (Brachylaemus) Dujardin, 1843 (Trematoda). Mem Inst Oswaldo Cruz 65:41–61

    Article  Google Scholar 

  • Gardner SL, Campbell ML (1992) Parasites as probes for biodiversity. J Parasitol 78:596–600

    Article  CAS  Google Scholar 

  • Gentile R, Fernandez FAS (1999) Influence of habitat structure on a streamside small mammal community in a Brazilian rural area. Mamm 63:29–10

    Article  Google Scholar 

  • Gentile R, Cardoso TS, Costa-Neto SF, Teixeira BR, D’Andrea PS (2018) Community structure and population dynamics of small mammals in an urban-Sylvatic interface area in Rio de Janeiro, Brazil. Zoo 35:1–12. https://doi.org/10.3897/zoologia.35.e13465

    Article  Google Scholar 

  • Gomes APN, Maldonado A, Bianchi RC, Souza JGR, D’Andrea PS, Gompper ME, Ollifiers N (2019) Variation in the prevalence and abundance of acanthocephalans in brown-nosed coatis Nasua nasua and crab-eating foxes Cerdocyon thous in the Brazilian Pantanal. Braz J Biol 79.https://doi.org/10.1590/1519-6984.187881

  • Gomes CD, Cruz PR, Vicente JJ, Pinto RM (2003) Nematode parasites of marsupials and small rodents from the Brazilian Atlantic Forest in the State of Rio de Janeiro, Brazil. Rev Bras Zoo 20:699–707. https://doi.org/10.1590/S0101-81752003000400024

    Article  Google Scholar 

  • Guégan JF, Lambert A, Lévêque C, Combes C, Euzet L (1992) Can host body size explain the parasite species richness in tropical freshwater fishes? Oecol 90:197–204. https://doi.org/10.1007/BF00317176

    Article  Google Scholar 

  • Gregory RD, Keymer AE, Harvey PH (1996) Helminth parasite richness among vertebrates. Biodivers Conserv 5:985–997

    Article  Google Scholar 

  • Hammer O, Harper DA, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron 4:9

    Google Scholar 

  • Heukelbach J, Feldmeier H (2008) Epidemiological and clinical characteristics of hookworm-related cutaneous larva migrans. Lancet Infect Dis 8:302–309. https://doi.org/10.1016/S1473-3099(08)70098-7

    Article  PubMed  Google Scholar 

  • Jansen AM, Xavier SCC, Roque ALR (2015) The multiple and complex and changeable scenarios of the Trypanosoma cruzi transmission cycle in the sylvatic environment. Acta Trop 151:1–15. https://doi.org/10.1016/j.actatropica.2015.07.018

    Article  PubMed  Google Scholar 

  • Jiménez FA, Catzeflis F, Gardner SL (2011) Structure of parasite component communities of Didelphis marsupialis: insights from a comparative study. J Parasitol 97:779–787. https://doi.org/10.1645/GE-2711.1

    Article  PubMed  Google Scholar 

  • Kajin M, Cerqueira R, Vieira MV, Gentile R (2008) Nine-year demography of the black-eared opossum Didelphis aurita (Didelphimorphia: Didelphidae) using life tables. Rev Bras Zoo 25:206–213. https://doi.org/10.1590/S0101-81752008000200007

    Article  Google Scholar 

  • Lima MM, Sarquis O, Oliveira TG, Gomes TF, Coutinho C, Teixeira NFD, Toma HK, Britto C, Teixeira BR, D’Andrea PS, Jansen AM, Bóia MN, Costa FAC (2012) Investigation of Chagas disease in four periurban areas in northeastern Brazil: epidemiologic survey in man, vectors, non-human hosts and reservoirs. Trans R Soc Trop Med Hyg 106:143–149. https://doi.org/10.1016/j.trstmh.2011.10.013

    Article  PubMed  Google Scholar 

  • Lee SH, Hwang SW, Chai JY, Seo BS (1984) Comparative morphology of eggs of heterophyids and Clonorchis sinensis causing human infections in Korea. Korean J Parasitol 22:171–180

    Article  Google Scholar 

  • Loreau M, Roy J, Tilman D (2005) Linking ecosystem and parasite ecology. In: Thomas F, Renaud F, Guégan JF (eds) Parasitism and ecosystems, 1st end. Oxford University Press, London, pp 13–21

    Chapter  Google Scholar 

  • Mittermeier RA, Gil RP, Hoffman M, Pilgrim J, Brooks T, Mittermeier CG, Lamoreux J, Fonseca GAB (2005) Hotspots revisited: earth’s biologically richest and most endangered terrestrial ecoregions, 2nd edn. University of Chicago Press (Boston), Conservation International

  • Monteiro RV, Dietz JM, Raboy B, Beck B, Vleeschower KD, Baker A, Martins A, Jansen AM (2007) Parasite community interactions: Trypanosoma cruzi and intestinal helminths infecting wild golden lion tamarins Leontopithecus rosalia and golden-headed lion tamarins L. chrysomelas (Callitrichidae, L., 1766). Parasitol Res 101:1689–1698. https://doi.org/10.1007/s00436-007-0652-2

    Article  PubMed  Google Scholar 

  • Morand S, Harvey PH (2000) Mammalian metabolism, longevity and parasite species richness. Proc R Soc 267:1999–2003. https://doi.org/10.1098/rspb.2000.1241

    Article  CAS  Google Scholar 

  • Oksanen J, Guillaume Blanchet F, Friendly M, Kindt R, Legendre P, McGlinn D, Minchin PR, O’Hara RB, Simpson GL, Solymos P, Stevens MHH, Szoecs E, Wagner H (2019) vegan: Community Ecology Package. R package version 2.5–6

  • Oliveira-Menezes A, Lanfredi-Rangel A, Lanfredi RM (2011) The first description of eggs in the male reproductive system of Physaloptera bispiculata (Nematoda: Spiruroidaea). J Helminthol 85:142–145. https://doi.org/10.1017/S0022149X10000374

    Article  CAS  PubMed  Google Scholar 

  • Peig J, Green AJ (2009) New perspectives for estimating body condition from mass/length data: the scaled mass index as an alternative method. Oikos 118:1883–1891. https://doi.org/10.1111/j.1600-0706.2009.17643.x

    Article  Google Scholar 

  • Poulin R, George-Nascimento M (2007) The scaling of total parasite biomass with host body mass. Internat J Parasitol 37:359–364. https://doi.org/10.1016/j.ijpara.2006.11.009

    Article  Google Scholar 

  • Püttker T, Meyer-Lucht Y, Sommer S (2008) Effects of fragmentation on parasite burden (nematodes) of generalist and specialist small mammal species in secondary forest fragments of the coastal Atlantic Forest. Brazil Ecol Res 23:207–215. https://doi.org/10.1007/s11284-007-0366-z

    Article  Google Scholar 

  • Ramos-de-Souza J, Maldonado-Jr A, Vilela RV, Andrade-Silva BE, Barbosa HS, Gomes SR, Thiengo SC (2021) First report of the nematode Cruzia tentaculata using molluscs as natural intermediate hosts, based on morphology and genetic markers. Inter J Parasitol: Parasite Wildl 15:105–111. https://doi.org/10.1016/j.ijppaw.2021.02.013

    Article  Google Scholar 

  • Rangel FCS, Gomes, SR, Canuto T, Rodrigues OS, Thiengo SC (2021) Diversity of non-marine gastropods of the Fiocruz Atlantic Forest Biological Station and adjacentes urban áreas, Rio de Janeiro, RJ, Brasil. An Acad Bras Cienc 93.https://doi.org/10.1590/0001-3765202120190691

  • R Core Team (2020) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria 

  • Richardson DJ, Gardner SL, Allen JW (2014) Redescription of Oligacanthorhynchus microcephalus (Rudolphi, 1819) Schmidt 1972 (syn. Oligacanthorhynchus tortuosa (Leidy, 1850) Schmidt 1972) (Acanthocephala: Oligacanthorhynchidae). Comparat Parasitol 81:53–60. https://doi.org/10.1654/4673.1

    Article  Google Scholar 

  • Robles MR, Cutillas C, Panei CJ, Callejón R (2014) Morphological and molecular characterization of a new Trichuris species (Nematoda-Trichuridae), and phylogenetic relationships of Trichuris species of Cricetid rodents from Argentina. PLoS ONE 11:1–11. https://doi.org/10.1371/journal.pone.0112069

    Article  CAS  Google Scholar 

  • Santori RT, Lessa LG, Astúa-de-Moraes D (2012) Alimentação, nutrição e adaptações alimentares de marsupiais brasileiros. In: Cáceres NC (ed) Os marsupiais do Brasil: Biologia, Ecologia e Conservação. 2nd edn. Editora UFMS, Campo Grande, pp 383–404

  • Schulte-Hostedde AI, Millar JS, Hickling GJ (2001) Evaluating body condition in small mammals. Can J Zoo 79:1021–1029. https://doi.org/10.1139/cjz-79-6-1021

    Article  Google Scholar 

  • Schulte-Hostedde AI, Zinner B, Millar JS, Hickling GJ (2005) Restitution of mass-size residuals: validating body condition indices. Ecol 86:155–163. https://doi.org/10.1890/04-0232

    Article  Google Scholar 

  • Sheather AL (1923) The detection of intestinal protozoa and mange parasites by a floatation technique. J Pathol Ther 36:266–275

    Article  Google Scholar 

  • Silva MGQ, Costa HMA (1999) Helminths of White-bellied opossum from Brazil. J Wildl Dis 35:371–374. https://doi.org/10.7589/0090-3558-35.2.371

    Article  Google Scholar 

  • Silva GM, Santos MB, Melo CM, Jeraldo VLS (2020) Achatina fulica (Gastropoda:Pulmonata): ocurrence, environmental aspects and presence of nematodes in Sergipe, Brasil. Braz J Biol. https://doi.org/10.1590/1519-6984.190291

    Article  PubMed  Google Scholar 

  • Taylor MA, Coop RL, Wall RL (2017) Parasitologia Veterinária, 4th end. Guanabara Koogan, Rio de Janeiro

    Google Scholar 

  • Teodoro AKM, Cutolo AA, Motoie G, Meira-Strejevitch CS, Pereira-Chioccola VL, Mendes TMF, Allegretti SM (2019) Gastrointestinal, skin and blood parasites in Didelphis spp. from urban and sylvatic areas in São Paulo state, Brazil. Vet Parasitol Reg Stud Rep. https://doi.org/10.1016/j.vprsr.2019.100286

    Article  Google Scholar 

  • Thatcher VE (2006) Endoparasitos dos marsupiais brasileiros. In: Cáceres N, Monteiro Filho E (eds) Os marsupiais do Brasil: biologia ecologia e evolução, 1st edn. Editora UFMS, Campo Grande, pp 53–68

    Google Scholar 

  • Travassos L (1917) Contribuições para o conhecimento da fauna helmintológica brasileira. VI Revisão dos acantocefalos brasileiros. Parte i Mem Inst Oswaldo Cruz 9:5–62

    Article  Google Scholar 

  • Travassos LP (1937) Revisão da família Trichostrongylidae Leiper, 1912. Monography, Instituto Oswaldo Cruz

  • Vicente JJ, Rodrigues HO, Gomes DC, Pinto RM (1997) Nematoides do Brasil. Parte V: Nematoides de mamíferos. Rev Bras Zoo 14:452

    Article  Google Scholar 

  • Vicente JJ, Rodrigues HO, Steindel M, Padovani CR, Schlemper JRBR (1987) Contribuição ao conhecimento da fauna helmintológica de mamíferos da Ilha de Santa Catarina, SC. At Soc Biol Rio De Janeiro 27:5–8

    Google Scholar 

  • Zabott MV, Pinto SB, Viott AM, Gruchouskei L, Bittencourt LHFB (2017) Helmintofauna de Didelphis albiventris (Lund, 1841) no município de Palotina Paraná, Brasil. Arq de Ciênc Vet Zool UNIPAR 20:19–22. https://doi.org/10.25110/arqvet.v20i1.6315

    Article  Google Scholar 

Download references

Acknowledgements

We would like to thank the staff and students of Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios and Laboratório de Biologia de Tripanossomatíedeos for helping in the field work, Dr Paulo D’Andrea for the ICMBio and INEA licences, the coordinator of Fiocruz Atlantic Forest Campus, Gilson Antunes, for providing local facilities and supporting the general project, and the team of Fiocruz Atlantic Forest Campus, especially to Dr. Ricardo Moratelli.

Funding

This work was financially supported by Instituto Oswaldo Cruz—IOC/FIOCRUZ, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro—FAPERJ (APQ1 E-26/010.001597/2019) and Programa de Pós-Graduação em Biodiversidade e Saúde (IOC-FIOCRUZ). RGB received PhD grants from Fundação Oswaldo Cruz. LM received undergraduate grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq. RG received researcher grants from CNPq (PQ-304355/2018–6).

Author information

Authors and Affiliations

  1. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-360, Brazil

    Raquel Gonzalez Boullosa, Leonardo Morgado, Arnaldo Maldonado Jr & Rosana Gentile

  2. Programa de Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, Manguinhos, Rio de Janeiro, RJ, 4365, 21040-360, Brazil

    Raquel Gonzalez Boullosa & Sócrates F. Costa-Neto

  3. Fundação Oswaldo Cruz, Campus Fiocruz Mata Atlântica, Rio de Janeiro, RJ, 22713-560, Brazil

    Sócrates F. Costa-Neto

Authors
  1. Raquel Gonzalez Boullosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sócrates F. Costa-Neto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Leonardo Morgado
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Arnaldo Maldonado Jr
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rosana Gentile
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

RGB, AMJ and RG contributed to the study conception and design. RGB, RG, SFCN and LM carried out the fieldwork. RGB, SFCN and LM analysed the samples collected. RGB and RG analysed the data. The first draft of the manuscript was written by RGB and RG. All authors contributed to the final version of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Raquel Gonzalez Boullosa.

Ethics declarations

Ethics approval

The animals were captured under the authorization of the Chico Mendes Institute for Biodiversity and Conservation of the Brazilian Government (ICMBIO, license number 13373) and the Environmental Institute of the state of Rio de Janeiro (INEA, license 020/2011). All procedures followed the guidelines for capture, handling and care of animals of the Ethics Committee on Animal Use of the Oswaldo Cruz Foundation (CEUA license number LW-39/14 and L-036/2018).

Consent to participate

Not applicable.

Consent for publication

Not applicable.

Conflict of interest

The authors declare no competing interests.

Additional information

Section Editor: Abdul Jabbar

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Boullosa, R.G., Costa-Neto, S.F., Morgado, L. et al. A longitudinal survey of gastrointestinal parasites of the black-eared opossum Didelphis aurita at an urban-sylvatic interface area in Southeast Brazil: a morphological and ecological characterization based on helminth eggs. Parasitol Res 120, 3815–3825 (2021). https://doi.org/10.1007/s00436-021-07314-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-021-07314-5

Keywords

Search

Navigation