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Drug-induced reactive oxygen species–mediated inhibitory effect on growth of Trypanosoma evansi in axenic culture system

  • Protozoology - Original Paper
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Abstract

Trypanosoma evansi, an extracellular haemoflagellate, has a wide range of hosts receptive and susceptible to infection, in which it revealed highly inconsistent clinical effects. Drugs used for the treatment of trypanosomosis have been utilized for more than five decades and have several problems like local and systemic toxicity. In the present investigation, imatinib and sorafenib were selected as drugs as they are reported to have the potential to cause reactive oxygen species (ROS)–mediated effect in cancer cells. Both have also been reported to have potential against T. brucei, T. cruzi and Leishmania donovani. To date, imatinib and sorafenib have not evaluated for their growth inhibitory effect against T. evansi. Imatinib and sorafenib showed significant (p < 0.001) inhibition on parasite growth and multiplication with IC50 (50% inhibitory concentration) values 6.12 μM and 0.33 μM respectively against T. evansi. Both the drug molecules demonstrated for the generation of ROS in T. evansi and were found up to 65% increased level of ROS as compared with negative control in the axenic culture system. Furthermore, different concentrations of imatinib and sorafenib were found non-toxic on horse peripheral blood mononuclear cells and Vero cell lines. Also, in conclusion, our results demonstrated that imatinib- and sorafenib-induced generation of ROS contributed inhibitory effect on the growth of Trypanosoma evansi in an axenic culture system.

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Acknowledgements

The authors gratefully thank the Director, ICAR-National Research Centre on Equines, Hisar, India, for providing all the necessary facilities for conducting this study.

Funding

The financial support from ICAR-New Delhi under the National Fellow Project is duly acknowledged.

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Authors and Affiliations

  1. Parasitology Lab, ICAR-National Research Centre on Equine, Hisar, Haryana, 125001, India

    Rajender Kumar, Ruma Rani, Khushboo Sethi, Shikha Jain, Sanjay Kumar & B. N. Tripathi

  2. Faculty of Veterinary & Animal Sciences, Banaras Hindu University, RGSC, Barkachha, Mirzapur, Uttar Pradesh, India

    Saroj Kumar

  3. Department of Animal Biotechnology, LLR University of Veterinary and Animal Sciences, Hisar, Haryana, 125001, India

    Kanisht Batra

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  1. Rajender Kumar
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  8. B. N. Tripathi
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Correspondence to Rajender Kumar.

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Prior approval was taken for animal experimentation in the present study from Institutional Animal Ethics Committee of ICAR-NRCE, Hisar.

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Kumar, R., Rani, R., Kumar, S. et al. Drug-induced reactive oxygen species–mediated inhibitory effect on growth of Trypanosoma evansi in axenic culture system. Parasitol Res 119, 3481–3489 (2020). https://doi.org/10.1007/s00436-020-06861-7

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  • DOI: https://doi.org/10.1007/s00436-020-06861-7

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