Vermamoeba vermiformis as etiological agent of a painful ulcer close to the eye
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In the present article, we report on the identification of Vermamoeba (Hartmannella) vermiformis as the etiological agent of a tissue infection close to the eye of a female patient. Laboratory examination revealed no involvement of any pathogenic bacteria or fungi in the tissue infection. V. vermiformis was identified by cultivation and morphology of trophozoites and cysts as well as phylogenetic analysis of nuclear 18S rDNA. The lesion improved in the course of 4 weeks by application of zinc paste.
KeywordsFree-living amoebae Vermamoeba vermiformis Hartmannella vermiformis Tissue infection
The author would like to thank Dr. David Lam (MD, MPH, Shaman Medical Consulting) for review and English language editing of the article and S. Njul for the technical assistance.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Abedkhojasteh H, Niyyati M, Rahimi F, Heidari M, Farnia S, Rezaeian M (2013) First report of Hartmannella keratitis in a cosmetic soft contact lens wearer in Iran. Iran J Parasitol 8:481–485Google Scholar
- Adl SM, Bass D, Lane CE et al (2019) Revision to the classification, nomenclature, and diversity of eukaryotes. J Eukaryot Micobiol 66:4–119Google Scholar
- Balczun C, Scheid P (2017) Free-living amoebae as hosts for and vectors of intracellular microorganisms with public health significance. Viruses 9:E65Google Scholar
- Brieland JK, Fantone JC, Remick DG, LeGendre M, McClain M, Engleberg NC (1997) The role of Legionella pneumophila - infected Hartmannella vermiformis as an infectious particle in a murine model of Legionaires’ disease. Infect Immun 65:5330–5333Google Scholar
- Cabello-Vílchez A, Mena R, Zuñiga J, Cermeño P, Martín-Navarro C, González A, López-Arencibia A, Reyes-BatlleM PJE, Valladares B, Lorenzo-Morales J (2014) Endosymbiotic Mycobacterium chelonae in a Vermamoeba vermiformis strain isolated from the nasal mucosa of an HIV patient in Lima, Peru. Exp Parasitol 145(Suppl):S127–S130CrossRefGoogle Scholar
- De Jonckheere JF, Brown S (1998b) Is the free-living ameba Hartmannella causing keratitis? Clin Infect Dis 27:1337–1338Google Scholar
- Masangkay F, Milanez G, Karanis P, Nissapatorn V (2018) Vermamoeba vermiformis—global trend and future perspective. In: Reference module in earth systems and environmental sciences. https://doi.org/10.1016/b978-0-12-409548-9.11005-x
- Nicholas K, Nicholas H, Deerfield D (1997) GeneDoc: analysis and visualization of genetic variation. Embnew News 4:14Google Scholar
- Page FC (1988) A new key to freshwater and soil Gymnamoebae with instructions for culture. Freshwater Biological Association, Ambleside, UKGoogle Scholar
- Page FC (1991) Nackte Rhizopoda. In: Page FC, Siemensma FJ (eds) Nackte Rhizopoda und Heliozoa, Protozoenfauna Band 2. Gustav Fischer Verlag, Stuttgart, New York, pp 7–170Google Scholar
- Rohr U, Weber S, Michel R, Selenka F, Wilhelm M (1998) Comparison of free-living amoebae in hot water systems of hospitals with isolates from moisty sanitary areas by identifying genera and determining temperature tolerance. Appl Environ Microbiol 64:1822–1824Google Scholar
- Scheid P (2018) Free-living amoebae and their multiple impacts on environmental health. In: Encyclopedia of environmental health, 2nd Edtion. https://doi.org/10.1016/B978-0-12-409548-9.10969-8
- Tyson J, Pearce M, Vargas P, Bagchi S, Mulhern B, Cianciotto N (2013) Multiple Legionella pneumophila type II secretion substrates, including a novel protein, contribute to differential infection of the amoebae Acanthamoeba castellanii, Hartmannella vermiformis, and Naegleria lovaniensis. Infect Immun 81:1399–1410CrossRefGoogle Scholar
- Wadowsky RM, Butler LJ, Cook MK, Verma SM, Paul MA, Fields BS, Keleti G, Sykora JL, Yee RB (1988) Growth-supporting activity for Legionella pneumophila in tap water cultures and implication of hartmannellid amoebae as growth factors. Appl Environ Microbiol 54:2677–2682Google Scholar