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Pathological and molecular characterization of avian malaria in captive Magellanic penguins (Spheniscus magellanicus) in South America

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Abstract

Avian malaria is a mosquito-borne disease that affects multiple avian species and is caused by protozoans of the genus Plasmodium. An avian malaria infection caused by Plasmodium sp. in Magellanic penguins (Spheniscus magellanicus) with high mortality is described in a zoo in Southern Brazil. Clinically, three birds presented signs of inappetence, anorexia, pale mucosa, dyspnea, and opisthotonus, with death in a clinical course of 5–8 h. At the necropsy, all birds exhibited pale mucosa, marked splenomegaly and hepatomegaly, in addition to moderate leptomeningeal blood vessels ingurgitation in the brain. Microscopically, multiple exoerythrocytic meronts were observed in the cytoplasm of endothelial cells in the spleen, liver, heart, lungs, brain, kidneys, and pancreas. The spleen had a multifocal perivascular inflammatory infiltrate of lymphocytes, plasma cells, and macrophages, which also exhibited hemosiderosis and erythrophagocytosis. The liver had a multifocal periportal inflammatory infiltrate of lymphocytes, macrophages, and plasma cells, in addition to marked hemosiderosis in the hepatic sinusoids. Fragments of spleen, liver, brain, skeletal muscle, and lung were tested by the polymerase chain reaction technique for the detection of a fragment of the cytochrome B gene from haemosporidians, which resulted positive for Plasmodium spp. After sequencing, the samples were phylogenetically associated to Plasmodium sp. detected in Turdus albicollis (KU562808) in Brazil and matched to the lineage TURALB01 previously detected in T. albicollis. Avian malaria infections caused by Plasmodium sp. of lineage TURALB01 may occur in S. magellanicus with high mortality, and, thus, it is essential to detect and characterize the agent involved to obtain the differential diagnosis of the condition.

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Authors and Affiliations

  1. Departamento de Patologia Clínica Veterinária, Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9090, Prédio 42505, Porto Alegre, RS, 91540-000, Brazil

    Paula Augusto Taunde, Matheus Viezzer Bianchi, Fernando Soares da Silva, Tainã Normanton Guim, David Driemeier & Saulo Petinatti Pavarini

  2. Departamento de Patologia Veterinária, Laboratório de Imunoparasitologia, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade do Estado de São Paulo (UNESP), Via de Acesso Prof. Paulo Donato Castellani, Jaboticabal, SP, 14884-900, Brazil

    Lívia Perles & Marcos Rogério André

  3. GramadoZoo, Gramado, RS, Brazil

    Renan Alves Stadler

Authors
  1. Paula Augusto Taunde
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  2. Matheus Viezzer Bianchi
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  3. Lívia Perles
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  4. Fernando Soares da Silva
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  5. Tainã Normanton Guim
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  6. Renan Alves Stadler
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  7. Marcos Rogério André
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  8. David Driemeier
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  9. Saulo Petinatti Pavarini
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Correspondence to Matheus Viezzer Bianchi.

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The authors declared that there was explicit owner informed consent for inclusion of animals in this study. All co-authors approved the manuscript and its submission to the journal.

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The authors declare that they have no conflicts of interest.

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Section Editor: Larissa Howe

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Taunde, P.A., Bianchi, M.V., Perles, L. et al. Pathological and molecular characterization of avian malaria in captive Magellanic penguins (Spheniscus magellanicus) in South America. Parasitol Res 118, 599–606 (2019). https://doi.org/10.1007/s00436-018-6155-5

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  • DOI: https://doi.org/10.1007/s00436-018-6155-5

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