Abstract
Our previous studies report epidemics of non-tsetse-transmitted equine trypanosomosis in Mongolia. However, the current status of non-tsetse-transmitted equine trypanosomosis endemicity remains to be clarified in some parts of Mongolia. We previously reported the potential application of rTeGM6-4r-based diagnostic tools, an rTeGM6-4r-based immunochromatographic test (ICT) and an enzyme-linked immunosorbent assay (ELISA), in the serological surveillance of equine trypanosomosis in Mongolia. In the present study, the utility of the rTeGM6-4r-based ICT was validated. The rTeGM6-4r-based ICT accurately diagnosed positive reference sera that had been prepared from dourine horses in Mongolia, similarly to the rTeGM6-4r-based ELISA. The diagnostic performance of the rTeGM6-4r-based ICT was maintained when the strips were preserved for at least 2 months under dry conditions. The ICT detected 42 positive serum samples from a total of 1701 equine sera that had been collected from all 21 provinces of Mongolia. The κ-value, sensitivity and specificity of rTeGM6-4r-based ICT were 0.58, 50.0% (95% CI, 37.7–62.3%) and 99.3% (95% CI, 98.7–99.6%), respectively, in comparison to the rTeGM6-4r-based ELISA. Our field-friendly rTeGM6-4r-based ICT was found to be useful for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia.
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Acknowledgments
We thank Ms. Noriko Endo, Ms. Bat-Uyanga Lhagva and Ms. Shoko Hayakawa for their project management in Mongolia. We thank all of the Mongolian SATREPS project researchers for supporting the present study. We also thank Ms. Yoko Matsushita for her excellent technical assistance. This study was financially supported by the Japan Society for the Promotion of Science (JSPS), Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and AMED/JICA SATREPS (17jm0110006h0005).
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Supplemental Figure 1
The appearance of the rTeGM6-4r-based ICT strips failed and succeeded to detect the control line. These strips were used for the analysis of field samples in Mongolia. Representative strips that failed or succeeded to detect the control line are shown. (PDF 115 kb)
Supplemental Figure 2
The detection thresholds of the rTeGM6-4r-based ELISA and the ICT. The detection thresholds of the rTeGM6-4r-based ELISA and ICT when the tests were applied to positive serum samples diluted 2- to 4096-times. The red dashed line shows the cut-off (0.135) ELISA value. The detection thresholds of the ELISA and ICT were 2048-times dilution and 64-times dilution, respectively. (PDF 104 kb)
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Mizushima, D., Amgalanbaatar, T., Davaasuren, B. et al. The utility of an rTeGM6-4r-based immunochromatographic test for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia. Parasitol Res 117, 2913–2919 (2018). https://doi.org/10.1007/s00436-018-5982-8
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DOI: https://doi.org/10.1007/s00436-018-5982-8