Abstract
Schistosoma japonicum as a pathogeny requires dendritic cells to activate immune response. So, the research is to study the dynamic changes of CD3e−CD11c+ dendritic cells in mice infected with S. japonicum. Zero, 7, 28, 35, and 63 days were selected to study the variation of dendritic cells, and the proportions of CD3e−CD11c+ dendritic cells and CD86+ mature dendritic cells in spleens and bone marrow were tested by flow cytometry. As a result, the variation trends of dendritic cells in spleen and bone marrow are similar as follows: the proportions of CD3e−CD11c+ dendritic cells increased first and then decreased from day 35, but the percentages of CD86+ mature dendritic cells decreased from day 28 and increased in day 63. In vitro, cultured dendritic cells treated with SEA and SAWA were tested by flow cytometry, the variation trends of CD86 on dendritic cells are consistent with the results in days 28 and 63. Besides CD86, the expression of MHC-II also hints immune regulation. In conclusion, it is speculated that dendritic cells play a role of immune regulation through MHC-II and CD86 in S. japonicum infection. Immune regulation of dendritic cells is not only in favor of the survival of host and parasite but also can be used in the therapy for immune diseases.
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Abbreviations
- SEA:
-
Soluble egg antigen
- SAWA:
-
Soluble adult worm antigen
- DC:
-
Dendritic cells
- MHC-II:
-
Major histocompatibility complex II
References
Alloatti A, Kotsias F, Magalhaes JG, Amigorena S (2016) Dendritic cell maturation and cross-presentation: timing matters! Immunological reviews 272:97–108. doi:10.1111/imr.12432
Banchereau J et al (2000) Immunobiology of dendritic cells. Annual review of immunology 18:767–811. doi:10.1146/annurev.immunol.18.1.767
Carvalho L, Sun J, Kane C, Marshall F, Krawczyk C, Pearce EJ (2009) Review series on helminths, immune modulation and the hygiene hypothesis: mechanisms underlying helminth modulation of dendritic cell function. Immunology 126:28–34. doi:10.1111/j.1365-2567.2008.03008.x
Cella M, Sallusto F, Lanzavecchia A (1997) Origin, maturation and antigen presenting function of dendritic cells. Current opinion in immunology 9:10–16
Chai M et al (2006) Transcriptome profiling of lung schistosomula,in vitro cultured schistosomula and adult Schistosoma japonicum. Cellular and molecular life sciences : CMLS 63:919–929. doi:10.1007/s00018-005-5578-1
Cheng PC, Lin CN, Peng SY, Li LL, Luo TY, Fan CK, Lee KM (2013) A study of immunomodulatory genes responses to macrophages of Schistosoma japonicum infection during different stages by microarray analysis. Acta tropica 127:251–260. doi:10.1016/j.actatropica.2013.05.012
Dudek AM, Martin S, Garg AD, Agostinis P (2013) Immature, semi-mature, and fully mature dendritic cells: toward a DC-cancer cells interface that augments anticancer immunity. Frontiers in immunology 4:438. doi:10.3389/fimmu.2013.00438
Duriancik DM, Hoag KA (2009) The identification and enumeration of dendritic cell populations from individual mouse spleen and Peyer’s patches using flow cytometric analysis. Cytometry Part A : the journal of the International Society for Analytical Cytology 75:951–959. doi:10.1002/cyto.a.20794
Hotez PJ, Bethony JM, Diemert DJ, Pearson M, Loukas A (2010) Developing vaccines to combat hookworm infection and intestinal schistosomiasis. Nature reviews Microbiology 8:814–826. doi:10.1038/nrmicro2438
Hussaarts L, Yazdanbakhsh M, Guigas B (2014) Priming dendritic cells for th2 polarization: lessons learned from helminths and implications for metabolic disorders. Frontiers in immunology 5:499. doi:10.3389/fimmu.2014.00499
Kim WS, Kim H, Kwon KW, Im SH, Lee BR, Ha SJ, Shin SJ (2016) Cisplatin induces tolerogenic dendritic cells in response to TLR agonists via the abundant production of IL-10, thereby promoting Th2- and Tr1-biased T-cell immunity. Oncotarget. doi:10.18632/oncotarget.9260
Li J et al (2013) Cyclophilin A from Schistosoma japonicum promotes a Th2 response in mice. Parasites & vectors 6:330. doi:10.1186/1756-3305-6-330
Liu JY et al (2011) Adoptive transfer of dendritic cells isolated from helminth-infected mice enhanced T regulatory cell responses in airway allergic inflammation. Parasite immunology 33:525–534. doi:10.1111/j.1365-3024.2011.01308.x
Lutz MB, Schuler G (2002) Immature, semi-mature and fully mature dendritic cells: which signals induce tolerance or immunity? Trends in immunology 23:445–449
Manicassamy S, Pulendran B (2011) Dendritic cell control of tolerogenic responses. Immunological reviews 241:206–227. doi:10.1111/j.1600-065X.2011.01015.x
Phythian-Adams AT et al (2010) CD11c depletion severely disrupts Th2 induction and development in vivo. The Journal of experimental medicine 207:2089–2096. doi:10.1084/jem.20100734
Reis e Sousa C (2006) Dendritic cells in a mature age. Nature reviews Immunology 6:476–483. doi:10.1038/nri1845
Ricart E, Panes J, Benitez-Ribas D (2011) Dendritic cells: a new horizon in cell therapy for inflammatory bowel disease? Gastroenterologia y hepatologia 34:100–106. doi:10.1016/j.gastrohep.2010.10.011
Rollinson D et al (2013) Time to set the agenda for schistosomiasis elimination. Acta tropica 128:423–440. doi:10.1016/j.actatropica.2012.04.013
Stadecker MJ, Asahi H, Finger E, Hernandez HJ, Rutitzky LI, Sun J (2004) The immunobiology of Th1 polarization in high-pathology schistosomiasis. Immunological reviews 201:168–179. doi:10.1111/j.0105-2896.2004.00197.x
Takakura K, Kajihara M, Ito Z, Ohkusa T, Gong J, Koido S (2015) Dendritic-tumor fusion cells in cancer immunotherapy. Discovery medicine 19:169–174
Terrazas CA, Terrazas LI, Gomez-Garcia L (2010) Modulation of dendritic cell responses by parasites: a common strategy to survive. Journal of biomedicine & biotechnology 2010:357106. doi:10.1155/2010/357106
Tian F et al (2015) B10 cells induced by Schistosoma japonicum soluble egg antigens modulated regulatory T cells and cytokine production of T cells. Parasitology research 114:3827–3834. doi:10.1007/s00436-015-4613-x
Wells JW, Darling D, Farzaneh F, Galea-Lauri J (2005) Influence of interleukin-4 on the phenotype and function of bone marrow-derived murine dendritic cells generated under serum-free conditions. Scandinavian journal of immunology 61:251–259. doi:10.1111/j.1365-3083.2005.01556.x
Wilson NS et al (2003) Most lymphoid organ dendritic cell types are phenotypically and functionally immature. Blood 102:2187–2194. doi:10.1182/blood-2003-02-0513
Wynn TA, Thompson RW, Cheever AW, Mentink-Kane MM (2004) Immunopathogenesis of schistosomiasis. Immunological reviews 201:156–167. doi:10.1111/j.0105-2896.2004.00176.x
Xu X et al (2010) Activation-induced T helper cell death contributes to Th1/Th2 polarization following murine Schistosoma japonicum infection. Journal of biomedicine & biotechnology 2010:202397. doi:10.1155/2010/202397
Xu J, Zhang H, Chen L, Zhang D, Ji M, Wu H, Wu G (2014) Schistosoma japonicum infection induces macrophage polarization. Journal of biomedical research 28:299–308. doi:10.7555/jbr.27.20130072
Xu X, Gao X, Zhao X, Liao Y, Ji W, Li Q, Li J (2016) PU.1-silenced dendritic cells induce mixed chimerism and alleviate intestinal transplant rejection in rats via a Th1 to Th2 shift. Cellular Physiology and Biochemistry: international journal of experimental cellular physiology, biochemistry, and pharmacology 38:220–228. doi:10.1159/000438623
Zhang W, Yu X, Kwak M, Xu L, Zhang L, Yu Q, Jin JO (2016) Maturation of dendritic cells by pullulan promotes anti-cancer effect. Oncotarget. doi:10.18632/oncotarget.10183
Zhao QP, Jiang MS, Dong HF, Nie P (2012) Diversification of Schistosoma japonicum in Mainland China revealed by mitochondrial DNA. PLoS neglected tropical diseases 6:e1503. doi:10.1371/journal.pntd.0001503
Zhou H et al (2012) The secretions products from invading cercariae of S. japonicum (0-3hRP) restrain mouse dendritic cells to mature. Parasitology research 110:119–126. doi:10.1007/s00436-011-2458-5
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Thank you for the help from Professor Li He and Wei Hou and my partners. They have given me many suggestions in the process of revising the manuscript.
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The experiment was approved by the Animal Ethics Committee of Wuhan University, and the protocols were strictly in accordance with the rules. All mice were euthanatized on schedule.
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This research received no specific grant from any funding agency, commercial or not-for-profit sectors.
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Lin Chen and Qingzhou Chen are the first authors and contributed equally to this work.
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Chen, L., Chen, Q., Hou, W. et al. The dynamic changes of CD3e−CD11c+ dendritic cells in spleens and bone marrow of mice infected with Schistosoma japonicum . Parasitol Res 116, 1007–1011 (2017). https://doi.org/10.1007/s00436-017-5381-6
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DOI: https://doi.org/10.1007/s00436-017-5381-6