Skip to main content

Advertisement

SpringerLink
Log in

Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

In the poultry industry, Eimeria spp. is one of the important pathogens which cause significant economic losses. We have previously generated a chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for an antigen located in the apical cytoskeleton of Eimeria acervulina and with cross-reactive among Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp. Furthermore, the protein of Cryptosporidium parvum recognized by the 6D-12-G10 has been identified as elongation factor-1α (EF-1α). In the present study, to identify the target molecule of E. acervulina by the mAb, we performed two-dimensional Western blotting analysis. Finally, we found two positive molecules which are identified as EF-1α and a related protein. Our previous finding using C. parvum and the results in this study suggest that EF-1α could be associated with the invasion facilitated by the cytoskeleton at the apical region of zoites.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Becker M, Kuhse J, Kirsch J (2013) Effects of two elongation factor 1A isoforms on the formation of gephyrin clusters at inhibitory synapses in hippocampal neurons. Histochem Cell Biol 140:603–609

    Article  CAS  PubMed  Google Scholar 

  • Blackman MJ, Bannister LH (2001) Apical organelles of Apicomplexa: biology and isolation by subcellular fractionation. Mol Biochem Parasitol 117:11–25

    Article  CAS  PubMed  Google Scholar 

  • Choi WI, Kim Y, Kim Y, Yu MY, Park J, Lee CE, Jeon BN, Koh DI, Hur MW (2009) Eukaryotic translation initiator protein 1A isoform, CCS-3, enhances the transcriptional repression of p21CIP1 by proto-oncogene FBI-1 (Pokemon/ZBTB7A). Cell Physiol Biochem 23:359–370

    Article  CAS  PubMed  Google Scholar 

  • Constantinoiu CC, Lillehoj HS, Matsubayashi M, Hosoda Y, Tani H, Matsuda H, Sasai K, Baba E (2003) Analysis of cross-reactivity of five new chicken monoclonal antibodies which recognize the apical complex of Eimeria using confocal laser immunofluorescence assay. Vet Parasitol 118:29–35

    Article  CAS  PubMed  Google Scholar 

  • Dalloul RA, Lillehoj HS (2006) Poultry coccidiosis: recent advancements in control measures and vaccine development. Expert Rev Vaccines 5:143–163

    Article  CAS  PubMed  Google Scholar 

  • Doyle A, Crosby SR, Burton DR, Lilley F, Murphy MF (2011) Actin bundling and polymerisation properties of eukaryotic elongation factor 1 alpha (eEF1A), histone H2A-H2B and lysozyme in vitro. J Struct Biol 176:370–378

    Article  CAS  PubMed  Google Scholar 

  • Dubremetz JF, Garcia-Réguet N, Conseil V, Fourmaux MN (1998) Apical organelles and host-cell invasion by Apicomplexa. Int J Parasitol 28:1007–1013

  • Hatefi Y, Yamaguchi M (1996) Nicotinamide nucleotide transhydrogenase: a model for utilization of substrate binding energy for proton translocation. FASEB J 10:444–452

    CAS  PubMed  Google Scholar 

  • Hu K, Roos DS, Murray JM (2002) A novel polymer of tubulin forms the conoid of Toxoplasma gondii. J Cell Biol 156:1039–1050

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  • Lillehoj HS, Sasai K, Matsuda H (1994) Development and characterization of chicken-chicken B cell hybridomas secreting monoclonal antibodies that detect sporozoite and merozoite antigens of Eimeria. Poult Sci 73:1685–1693

    Article  CAS  PubMed  Google Scholar 

  • Matsubayashi M, Kimata I, Iseki M, Lillehoj HS, Matsuda H, Nakanishi T, Tani H, Sasai K, Baba E (2005) Cross-reactivities with Cryptosporidium spp. by chicken monoclonal antibodies that recognize avian Eimeria spp. Vet Parasitol 128:47–57

    Article  PubMed  Google Scholar 

  • Matsubayashi M, Takase H, Kimata I, Nakagawa H, Tani H, Sasai K, Baba E (2008) Electron microscopic observation of cytoskeletal frame structures and detection of tubulin on the apical region of Cryptosporidium parvum sporozoites. Parasitology 135:295–301

    Article  CAS  PubMed  Google Scholar 

  • Matsubayashi M, Teramoto-Kimata I, Uni S, Lillehoj HS, Matsuda H, Furuya M, Tani H, Sasai K (2013) Elongation factor-1α is a novel protein associated with host cell invasion and a potential protective antigen of Cryptosporidium parvum. J Biol Chem 288:34111–34120

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Morrissette NS, Sibley LD (2002) Cytoskeleton of apicomplexan parasites. Microbiol Mol Biol Rev 66:21–38

    Article  PubMed  PubMed Central  Google Scholar 

  • Ransom-Hodgkins WD (2009) The application of expression analysis in elucidating the eukaryotic elongation factor one alpha gene family in Arabidopsis thaliana. Mol Genet Genomics 281:391–405

    Article  CAS  PubMed  Google Scholar 

  • Ridgley EL, Xiong ZH, Kaur KJ, Ruben L (1996) Genomic organization and expression of elongation factor-1 alpha genes in Trypanosoma brucei. Mol Biochem Parasitol 79:119–123

    Article  CAS  PubMed  Google Scholar 

  • Riis B, Rattan SI, Clark BF, Merrick WC (1990) Eukaryotic protein elongation factors. Trends Biochem Sci 15:420–424

    Article  PubMed  Google Scholar 

  • Sasai K, Lillehoj HS, Hemphill A, Matsuda H, Hanioka Y, Fukata T, Baba E, Arakawa A (1998) A chicken anti-conoid monoclonal antibody identifies a common epitope which is present on motile stages of Eimeria, Neospora, and Toxoplasma. J Parasitol 84:654–656

    Article  CAS  PubMed  Google Scholar 

  • Sasai K, Lillehoj HS, Matsuda H, Wergin WP (1996) Characterization of a chicken monoclonal antibody that recognizes the apical complex of Eimeria acervulina sporozoites and partially inhibits sporozoite invasion of CD8+ T lymphocytes in vitro. J Parasitol 82:82–87

    Article  CAS  PubMed  Google Scholar 

  • Santos JM, Lebrun M, Daher W, Soldati D, Dubremetz JF (2009) Apicomplexan cytoskeleton and motors: key regulators in morphogenesis, cell division, transport and motility. Int J Parasitol 39:153–162

    Article  CAS  PubMed  Google Scholar 

  • Schmatz DM, Crane MS, Murray PK (1984) Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. J Protozool 31:181–183

    Article  CAS  PubMed  Google Scholar 

  • Stotish RL, Simashkevich PM, Wang CC (1977) Separation of sporozoites, sporocysts, and oocysts of Eimeria tenella by centrifugal elutriation. J Parasitol 63:1124–1126

    Article  CAS  PubMed  Google Scholar 

  • Vermeulen AN, Kok JJ, van den Boogaart P, Dijkema R, Claessens JA (1993) Eimeria refractile body proteins contain two potentially functional characteristics: transhydrogenase and carbohydrate transport. FEMS Microbiol Lett 110:223–229

    Article  CAS  PubMed  Google Scholar 

  • Yang F, Demma M, Warren V, Dharmawardhane S, Condeelis J (1990) Identification of an actin-binding protein from Dictyostelium as elongation factor 1a. Nature 347:494–496

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We are grateful to Dr. Y. Fukuta, from the APRO Life Science Institute, Inc., for the LC-MS/MS analyses. This study was supported in part by the Grants-in-aid for Scientific Research 23580445 (to K. S., H. T., M. F., and M. M.) and 25450436 (to M. M.) from the Ministry of Education, Culture, Sports, and Science.

Author information

Authors and Affiliations

  1. Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan

    Makoto Matsubayashi, Chisa Minoura, Shintaro Kimura, Hiroyuki Tani, Masaru Furuya, Shigeo Takenaka & Kazumi Sasai

  2. Animal Biosciences and Biotechnology Laboratory, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, 20705, USA

    Hyun S. Lillehoj

  3. Laboratory of Immunobiology, Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi, Hiroshima, 739-8528, Japan

    Haruo Matsuda

  4. Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa, 252-0374, Japan

    Takeshi Hatta & Naotoshi Tsuji

Authors
  1. Makoto Matsubayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chisa Minoura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shintaro Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroyuki Tani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masaru Furuya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hyun S. Lillehoj
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Haruo Matsuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Shigeo Takenaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Takeshi Hatta
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Naotoshi Tsuji
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Kazumi Sasai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kazumi Sasai.

Ethics declarations

Ethics statement

Any animals were not scarified for the purpose of this study. Any human participants were not involved in this study. All the authors have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Matsubayashi, M., Minoura, C., Kimura, S. et al. Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody. Parasitol Res 115, 4123–4128 (2016). https://doi.org/10.1007/s00436-016-5185-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-016-5185-0

Keywords

Search

Navigation