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Characterization of the complete mitochondrial genome sequence of Homalogaster paloniae (Gastrodiscidae, Trematoda) and comparative analyses with selected digeneans

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Abstract

Gastrodiscidae species are neglected but significant paramphistomes in small ruminants, which can lead to considerable economic losses to the breeding industry of livestock. However, knowledge about molecular ecology, population genetics, and phylogenetic analysis is still limited. In the present study, we firstly sequenced and analyzed the full mitochondrial (mt) genome of Homalogaster paloniae (14,490 bp). The gene contents and organization of the H. paloniae mt genome is the same as that of other digeneans, such as Fasciola hepatica and Paramphistomum cervi. It is interesting that unlike other paramphistomes, H. paloniae is flat in shape which is similar with Fasciola, such as F. hepatica. Phylogenetic analysis of H. paloniae and other 17 selected digeneans using concatenated amino acid sequences of the 12 protein-coding genes showed that Gastrodiscidae is closely related to Paramphistomidae and Gastrothylacidae. The availability of the mt genome sequence of H. paloniae should provide an important foundation for further molecular study of Gastrodiscidae and other digeneans.

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Acknowledgments

This work was supported by the “Special Fund for Agro-scientific Research in the Public Interest” (Grant No. 201303037), “National Key Basic Research Program (973Program) of China” (Grant No. 2015CB150300).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Xin Yang, Mingwei Qi, Zongze Zhang, Chong Gao, Chunqun Wang, Min Hu & Rui Fang

  2. Hubei Provincial Center for Diseases Control and Prevention, Wuhan, 430079, Hubei, China

    Lixia Wang

  3. Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan, 430022, Hubei, China

    Hanli Feng

  4. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Chengye Li

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  1. Xin Yang
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  2. Lixia Wang
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  3. Hanli Feng
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  4. Mingwei Qi
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  5. Zongze Zhang
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  7. Chunqun Wang
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  8. Min Hu
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  9. Rui Fang
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  10. Chengye Li
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Correspondence to Rui Fang.

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Yang, X., Wang, L., Feng, H. et al. Characterization of the complete mitochondrial genome sequence of Homalogaster paloniae (Gastrodiscidae, Trematoda) and comparative analyses with selected digeneans. Parasitol Res 115, 3941–3949 (2016). https://doi.org/10.1007/s00436-016-5160-9

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  • DOI: https://doi.org/10.1007/s00436-016-5160-9

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