Abstract
There is always controversy regarding identification of different species in the genus Eurytrema. Identification has been based mainly on morphology, which can be misleading and subject to differing interpretation among the scientists. Therefore, the aim of this study was to identify Eurytrema flukes both by morphology and molecular properties on the basis of 18-subunit ribosomal RNA (18S rRNA) gene as well as internal transcribed spacer 2 (ITS2) to clarify their phylogenetic status. Among six different agroecological areas of Bangladesh, 22 Eurytrema flukes were recovered from the bile ducts of 22 cattle in Bandarban, a hill district. The flukes were identified as Eurytrema cladorchis through morphometric and morphological studies. Phylogenetic analyses were conducted by neighbor-joining phylogram inferred from both 18S rRNA (1784 bp) gene and ITS2 (229 bp) sequences. A monophyletic clade was constructed by the E. cladorchis from Bangladesh; however, the clade was distinct from those formed by Eurytrema pancreaticum and Eurytrema coelomaticum. This study first described the existence of E. cladorchis from Bangladesh and may provide useful information for both morphological and molecular properties that may further help to clarify phylogenetic relationships within the genus Eurytrema and also for other digeneans.
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References
Blair D, Barker SC (1993) Affinities of the Gyliauchenidae: utility of the 18S rRNA genes for the phylogenetic inference in the Digenea (Platyhelminthes). Int J Parasitol 23:527–532
Cai Z, Zhang Y, Ye X (2012) Phylogenetic relationships of the genus Eurytrema from domestic and wild animal based on 18S rRNA sequences. Parasitol Res 111:1637–1644
Itagaki T, Tsutsumi K (1998) Triploid form of Fasciola in Japan: genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS–2 sequence of nuclear rDNA. Int J Parasitol 28:777–781
Jin DX, Li GZ, Wei CF (1965) A new species of Eurytrema in animal in Guizhou Province. Acta Parasitol Sinica 2:28–35
Jones A (1985) Eurytrema cladorchis Chin, Li and Wei, 1965 (Trematoda: Dicrocoelidae), a little known species from China and Nepal. Syst Parasitol 7:43–45
Kumar V (1998) Trematode infections and diseases of man and animals. Eurytremiasis. Kluwer Academic Publisher, Dordrecht, pp 230–233
Orosova M, Ivica KH, Eva B, Marta S (2010) Chromosomal characteristics of multiple rDNA clusters and intragenomic variability of ribosomal ITS2 in Caryophyllaeides fennica (Cestoda). Parasitol Int 59:351–357
Swofford DL (2001) PAUP: Phylogenetic analysis using parsimony and other methods ver. 4.0beta. Sanderland, Massachussetts: Sinauer Associates
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular evolution genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Tang CC (1950) Studies on the life history of Eurytrema pancreaticum Janson, 1889. J Parasitol 36:559–574
Tang CT, Lin TM (1980) Investigations of Eurytrematosis of cattle and goats in mountainous regions of North Fu–jian. Acta Zool Sin 26:42–51 (In Chinese )
Tang CC, Tang CT (1977) The biology and epidemiology of Eurytrema coelomaticum (Giard and Billet, 1892) and Eurytrema pancreaticum (Jansen, 1889) in cattle and sheep in China. Acta Zool Sin 23:267–282 (In Chinese )
Tang CC, Tang CT (1978) Investigation on trematodes belonging to the family Dicrocoelidae Odhner, 1911. J Xiamen Univ 4:64–80
Tang CT, Lin TM, Lin HM (1978) On the life cycle of Eurytrema cladorchis of cattle and goat in Pucheng, North Fujian. Xiamen Daxue Xuebao 14:104–117 (In Chinese)
Wiroreno W, Carney WP, Ansori M (1987) Description and growth pattern of Eurytrema pancreaticum from Bos indicus from East Java. Proc Helminthol Soc Wash 54:73–77
Yamada S, Yoshida A, Yoshida K, Kuraishi T, Hattori S, Kai C, Nagai Y, Sakoda T, Tatara M, Abe S, Fukumoto SI (2012) Phylogenetic relationships of three species within the family Heligmonellidae (Nematoda; Heligmosomoidea) from Japanese rodents and a lagomorph based on the sequences of ribosomal DNA internal transcribed spacers, ITS–1 and ITS–2. Jpn J Vet Res 60:15–21
Zheng YD, Luo XN, Shi CH, Zong RQ, Jing ZZ, Cai XP (2006) Molecular relationship of Eurytrema coelomaticum inferred from 18S rRNA sequence. Chin J Parasitol Parasit Dis 24:345–348 (in Chinese)
Zheng Y, Luo X, Jing Z, Hu Z, Cai X (2007) Comparison of 18S ribosomal gene sequences of Eurytrema coelomaticum and Eurytrema pancreaticum. Parasitol Res 100:645–646
Acknowledgments
This study was supported in part by a Grant-in-Aid for Science Research (B) and (C) (nos. 23405044, 24580420, 22405037) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Mohanta, U.K., Ichikawa-Seki, M., Hayashi, K. et al. Morphological and molecular characterization of Eurytrema cladorchis parasitizing cattle (Bos indicus) in Bangladesh. Parasitol Res 114, 2099–2105 (2015). https://doi.org/10.1007/s00436-015-4398-y
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DOI: https://doi.org/10.1007/s00436-015-4398-y