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Incidence and molecular diversity of Acanthamoeba species isolated from public baths in Hungary

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Abstract

Hungary has a large number of thermal baths and spa facilities which attract hundreds of thousands of tourists annually. Until recently, however, the free-living amoebae were not of public health concern. Genotyping of Acanthamoeba species, potential agents of keratitis and granulomatous encephalitis, was carried out in 20 Hungarian public baths for the first time to assess the incidence and molecular diversity of the genus in the country. Our results show that 6.7 % of the samples were positive for Acanthamoeba. Of these positive samples, 6.5 and 7 % was from sterilized and unsterilized pools, respectively. The 18S rRNA gene investigation of the nine Acanthamoeba strains found reveals that seven belong to the hazardous T4 genotype. The remaining two samples were of the T15 type. All the strains kept growing at 36 °C. Our results underline the need to develop a control system for free-living amoebae and supervise the disinfection of Hungarian public baths.

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References

  • Castellano-Sanchez A, Popp AC, Nolte FS, Visvesvara GS, Thigpen M, Redei I, Somani J (2003) Acanthamoeba castellani encephalitis following partially mismatched related donor peripheral stem cell transplantation. Transpl Infect Dis 5:191–194

    Article  CAS  PubMed  Google Scholar 

  • Caumo K, Rott MB (2011) Acanthamoeba T3, T4 and T5 in swimming-pool waters from Southern Brazil. Acta Trop 117:233–235

    Article  PubMed  Google Scholar 

  • Chan LL, Mak JW, Low YT, Koh TT, Ithoi I, Mohamed SM (2011) Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia. Acta Trop 117:23–30

    Article  PubMed  Google Scholar 

  • Corsaro D, Venditti D (2010) Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa. Acanthamoebida). Parasitol Res 107:233–238

    Article  PubMed  Google Scholar 

  • De Jonckheere JF (1979) Pathogenic free-living amebas in swimming pools—survey in Belgium. Ann Microbiol B 130(2):205–212

    Google Scholar 

  • Dykova I, Lom J, Schroeder-Diedrich JM, Booton GC, Byers TJ (1999) Acanthamoeba strains isolated from organs of freshwater fishes. J Parasitol 85:1106–1113

    Article  CAS  PubMed  Google Scholar 

  • Gast RJ (2001) Development of an Acanthamoeba-specific reverse dot-blot and the discovery of a new ribotype. J Eukaryot Microbiol 48:609–615

    Article  CAS  PubMed  Google Scholar 

  • Gast RJ, Ledee DR, Fuerst PA, Byers TJ (1996) Subgenus systematics of Acanthamoeba: four nuclear 18S rDNA sequence types. J Eukaryot Microbiol 43:498–504

    Article  CAS  PubMed  Google Scholar 

  • Hiti K, Walochnik J, Haller-Schober EM, Faschinger C, Aspöck H (2002) Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems. Br J Ophthalmol 86:144–146

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Horn M, Fritsche TR, Gautom RK, Schleifer KH, Wagner M (1999) Novel bacterial endosymbionts of Acanthamoeba spp. related to the Paramecium caudatum symbiont Caedibacter caryophilus. Environ Microbiol 1:357–367

    Article  CAS  PubMed  Google Scholar 

  • Jager BV, Stamm WP (1972) Brain abscesses caused by freeliving amoeba probably of the genus Hartmannella in a patient with Hodgkin’s disease. Lancet 2:1343–1345

    Article  CAS  PubMed  Google Scholar 

  • Khan NA (2006) Acanthamoeba: Biology and increasing importance in human health. FEMS Microbiol Rev 30:564–595

    Article  PubMed  Google Scholar 

  • Kilvington S, Price J (1990) Survival of Legionella pneumophilia within cysts of Acanthamoeba polyphaga following chlorine exposure. J Appl Bacteriol 68:519–525

    Article  CAS  PubMed  Google Scholar 

  • Kong HH (2009) Molecular Phylogeny of Acanthamoeba. Korean J Parasitol 47:S21–S28

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ledee DR, Iovieno A, Miller D, Mandal N, Diaz M, Fell J, Fini ME, Alfonso EC (2009) Molecular identification of T4 and T5 genotypes in isolates from Acanthamoeba keratitis patients. J Clin Microbiol 47:1458–1462

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lorenzo-Morales J, Morcillo-Laiz R, Martín-Navarro CM, López-Vélez R, López-Arencibia A, Arnalich-Montiel F, Maciver SK, Valladares B, Martínez-Carretero E (2010) Acanthamoeba keratitis due to genotype T11 in a rigid gas permeable contact lens wearer in Spain. Contact Lens Ant Eye 34:83–86

    Article  Google Scholar 

  • Maciver K, Asif M, Simmen W, Lorenzo-Morales J (2013) A systematic analysis of Acanthamoeba genotype frequency correlated with source and pathogenicity: T4 is confirmed as a pathogen-rich genotype. Eur J Protistol 49:217–221

    Article  PubMed  Google Scholar 

  • Mahmoudi MR, Taghipour N, Eftekhar M, Haghighi A, Karanis P (2012) Isolation of Acanthamoeba species in surface waters of Gilan province-north of Iran. Parasitol Res 110:473–477

    Article  PubMed  Google Scholar 

  • Martinez AJ (1982) Acanthamoebiasis and immunosuppression. J Neuropathol Exp Neurol 41:548–557

    Article  CAS  PubMed  Google Scholar 

  • Martinez A (1991) Infection of the central nervous system due to Acanthamoeba. Rev Infect Dis 13:399–402

    Article  Google Scholar 

  • Matyi A, Somogyi I, Deak J, Nagy E, Foldes J (1985) Acanthamoeba castellanii altal okozott meningoencephalitis magyarorszagi elofordulasa [Meningoencephalitis caused by Acanthamoeba castellanii in Hungary]. Orv Hetil 126:2541–2544

    CAS  PubMed  Google Scholar 

  • Nagington J, Watson PG, Playfair TJ, McGill J, Jones BR, Steele AD (1974) Amoebic infections of the eye. Lancet 2:1537–1540

    Article  Google Scholar 

  • Page FC (1988) A new key to freshwater and soil gymnamoebae. Freshwater Biological Association, Ambleside, 122 pp

    Google Scholar 

  • Penas-Ares M, Paniagua-Crespo E, Madrinan-Choren R, Marti-Mallen M, Arias-Fernandez MC (1994) Isolation of free-living pathogenic amoebae from thermal spas in N. W. Spain. Water Air Soil Poll 78(1–2):83–90

    Article  CAS  Google Scholar 

  • Pussard M, Pons R (1977) Morphologie de la paroi kystique et taxonomie du genre Acanthamoeba (Protozoa. Amoebida). Protistologica 13:557–598

    Google Scholar 

  • Qvarnstrom Y, Nerad TA, Visvesvara GS (2013) Charcterization of a new pathogenic Acanthamoeba species, A. byersi n.sp., isolated from a human with fatal amoebic encephalitis. J Eukaryot Microbiol 60:626–633

    Article  CAS  PubMed  Google Scholar 

  • Reyes-Batlle M, Todd CD, Martín-Navarro CM, López-Arencibia A, Cabello-Vilchez AM, González AC, Córdoba-Lanús E, Lindo JF, Valladares B, Piñero JE, Lorenzo-Morales J (2014) Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain. Parasitol Res. doi:10.1007/s00436-014-3778-z

    Google Scholar 

  • Rivera F, Lares F, Gallegos E, Ramírez E, Bonnila P, Calderón A, Martínez JJ, Rodríguez S, Alcocer J (1989) Pathogenic amebas in natural thermal waters of 3 resorts of Hidalgo, Mexico. Environ Res 50:289–295

    Article  CAS  PubMed  Google Scholar 

  • Sawyer T (1971) Acanthamoeba griffini a new species of marine amoeba. J Protozool 18:650–654

    Article  Google Scholar 

  • Scaglia M, Gatti S, Brustia R, Strosselli M, Bernuzzi AM, Cevini C (1987) Pathogenic and nonpathogenic Naegleria and Acanthamoeba spp—a new autochtonous isolate from an Italian thermal area. Microbiologica 10:171–182

    CAS  PubMed  Google Scholar 

  • Scheid PL, Schwarzenberger R (2011) Free-living amoebae as vectors of Cryptosporidia. Parasitol Res 109:499–504

    Article  PubMed  Google Scholar 

  • Scheid P, Schwarzenberger R (2012) Acanthamoeba spp. as vehicle and reservoir of adenoviruses. Parasitol Res 111:479–485

    Article  PubMed  Google Scholar 

  • Schuster FL, Visvesvara GS (2004) Free-living amoebae as opportunistic and nonopportunistic pathogens of humans and animals. Int J Parasitol 34:1001–1027

    Article  PubMed  Google Scholar 

  • Stockman LJ, Wright CJ, Visvesvara GS, Fields BS, Beach MJ (2011) Prevalence of Acanthamoeba spp. and other free-living amoebae in household water, Ohio, USA—1990–1992. Parasitol Res 108:621–627

    Article  PubMed  Google Scholar 

  • Stothard DR, Schroeder-Diedrich JM, Awwad MH, Gast RJ, Ledee DR, Rodriguez-Zaragoza S, Dean CL, Fuerst PA, Byers TJ (1998) The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J Eukaryot Microbiol 45:45–54

    Article  CAS  PubMed  Google Scholar 

  • Szénási Z, Endo T, Yagita K, Nagy E (1998) Isolation, identification and increasing importance of ‘free-living’ amoebae causing human disease. J Med Microbiol 47:5–16

    Article  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Trabelsi H, Dendana F, Sellami A, Sellami H, Cheikhrouhou F, Neji S, Makni F, Ayadi A (2012) Pathogenic free-living amoebae: Epidemiology and clinical review. Pathol Biol 60:399–405

    Article  CAS  PubMed  Google Scholar 

  • Tsvetkova N, Schild M, Panaiotov S, Kurdova-Mintcheva R, Gottstein B, Walochnik J, Aspöck H, Siles Lucas M, Müller N (2004) The identification of free-living environmental isolates of amoebae from Bulgaria. Parasitol Res 92:405–413

    Article  PubMed  Google Scholar 

  • Walochnik J, Haller-Schober EM, Kölli H, Picher O, Obwaller A, Aspöck H (2000) Discrimination between clinically relevant and nonrelevant Acanthamoeba strains isolated from contact lens-wearing keratitis patients in Austria. J Clin Microbiol 38:3932–3936

    CAS  PubMed Central  PubMed  Google Scholar 

  • Yu HS, Kong HH, Kim SY, Hahn YH, Hahn TW, Chung DI (2004) Laboratory investigation of Acanthamoeba lugdunensis from patients with keratitis. Invest Ophthalmol Vis Sci 45:1418–1426

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors wish to thank Mr. Robert F. Mattick for his grammatical English language review. Research was financed by the Hungarian National Scientific Foundation (OTKA) no. T-49632 project.

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  1. Government Office of Győr-Moson-Sopron County Policy Administration Services of Public Health, Győr, Hungary

    Csaba Kiss, Zsófia Barna, Márta Vargha & Júlia Katalin Török

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  1. Csaba Kiss
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  2. Zsófia Barna
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  4. Júlia Katalin Török
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Correspondence to Csaba Kiss.

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Kiss, C., Barna, Z., Vargha, M. et al. Incidence and molecular diversity of Acanthamoeba species isolated from public baths in Hungary. Parasitol Res 113, 2551–2557 (2014). https://doi.org/10.1007/s00436-014-3905-x

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