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Chemical composition and larvicidal activity of plant extracts from Clausena dentata (Willd) (Rutaceae) against dengue, malaria, and filariasis vectors

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Abstract

Mosquitoes in the larval stage are attractive targets for pesticides because mosquitoes breed in water, and thus, it is easy to deal with them in this habitat. The use of conventional pesticides in the water sources, however, introduces many risks to people and/or the environment. Natural pesticides, especially those derived from plants, are more promising in this aspect. Aromatic plants and their essential oils are very important sources of many compounds that are used in different respects. Insecticides of botanical origin may serve as suitable alternative to chemical insecticides. Acetone, chloroform, ethyl acetate, methanol, and petroleum benzine leaf extracts of Clausena dentata were tested against the fourth instar larvae of Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti (Diptera: Culicidae). Larval mortality was observed after 24 h of exposure. The highest larval mortality was found in acetone leaf extract, C. quinquefasciatus (LC50 = 0.150278 mg/ml; LC90 = 7.302613 mg/ml), A. aegypti (LC50 = 0.169495 mg/ml; LC90 = 1.10034 mg/ml), and A. stephensi (LC50 = 0.045684 mg/ml; LC90 = 0.045684 mg/ml). GC–MS analysis of plant extracts of acetone solvent revealed 16 compounds, of which the major compounds were benzene,1,2,3-trimethoxy-5-(2-propenyl) (14.97 %), Z,Z-6,28-heptatriactontadien-2-one (6.81 %), 2-allyl-4-methylphenol (28.14 %), 2-allyl-4-methylphenol (17.34 %), and 2,6,10,14,18,22-tetracosahexaene, 2,6,10,15,19,23-hexamethyl (10.35 %). Our result shows acetone leaf extracts of C. dentata have the potential to be used as an ideal eco-friendly approach for mosquito control.

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Acknowledgments

We would like to thank the VIT, Vellore, for providing the facility to carry out GC–MS analysis work. The financial support is provided by Indian Council of Medical Research- Vector Science Forum Major Research Project (F.No. 5/8-7 (335) V-2011-ECD-II, 21/06/2013). We also thank the Department of Biotechnology, Periyar University, Salem, for providing the infrastructural facility for carrying out this research work.

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Authors and Affiliations

  1. Molecular Entomology Lab, Department of Biotechnology, Periyar University, Salem, 636 011, Tamil Nadu, India

    Murugesan Susitra Manjari, Sengodan Karthi, Govindaraju Ramkumar, Ranganathan Muthusamy & Muthugoundar Subramanian Shivakumar

  2. Natural Drug Research Laboratory, Department of Biotechnology, Periyar University, Salem, 636 011, Tamil Nadu, India

    Devarajan Natarajan

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  1. Murugesan Susitra Manjari
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  2. Sengodan Karthi
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  3. Govindaraju Ramkumar
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  4. Ranganathan Muthusamy
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  6. Muthugoundar Subramanian Shivakumar
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Correspondence to Muthugoundar Subramanian Shivakumar.

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Manjari, M.S., Karthi, S., Ramkumar, G. et al. Chemical composition and larvicidal activity of plant extracts from Clausena dentata (Willd) (Rutaceae) against dengue, malaria, and filariasis vectors. Parasitol Res 113, 2475–2481 (2014). https://doi.org/10.1007/s00436-014-3896-7

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  • DOI: https://doi.org/10.1007/s00436-014-3896-7

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