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Evaluation of an I-box wind tunnel model for assessment of behavioral responses of blow flies

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Abstract

The behavioral response of flies to olfactory cues remains the focus of many investigations, and wind tunnels have sometimes been employed for assessment of this variable in the laboratory. In this study, our aim was to design, construct, and operate a new model of I-box wind tunnel with improved efficacy, highlighting the use of a new wind tunnel model to investigate the behavioral response of the medically important blow fly, Chrysomya megacephala (Fabricius). The I-box dual-choice wind tunnel designed for this study consists of seven conjoined compartments that resulted in a linear apparatus with clear glass tunnel of 30 × 30 × 190 cm ended both sides with wooden "fan compartments" which are equipped with adjustable fans as wind source. The clear glass tunnel consisted of two “stimulus compartments” with either presence or absence (control) of bait; two “trap compartments” where flies were attracted and allowed to reside; and one central “release compartment” where flies were introduced. Wind tunnel experiments were carried out in a temperature-controlled room, with a room light as a light source and a room-ventilated fan as odor-remover from tunnel out. Evaluation of testing parameters revealed that the highest attractive index was achieved with the use of 300 g of 1-day tainted pork scrap (pork meat mixed with offal) as bait in wind tunnel settings wind speed of 0.58 m/s, during 1.00–5.00 pm with light intensity of 341.33 lux from vertical light and 135.93 lux from horizontal light for testing a group of 60 flies. In addition, no significant response of well-fed and 24 h staved flies to this bait under these conditions was found. Results of this study supported this new wind tunnel model as a suitable apparatus for investigation of behavioral response of blow flies to bait chemical cues in the laboratory.

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References

  • Aak A, Knudsen GK, Soleng A (2010) Wind tunnel behavioral response and field trapping of the blowfly Calliphora vicina. Med Vet Entomol 24:250–257

    PubMed  CAS  Google Scholar 

  • Adams MR, Moss MO (1995) Food microbiology. New Age International Publishers, New Delhi, India

    Google Scholar 

  • Baker TC, Haynes KF (1989) Field and laboratory electroantennographic measurements of pheromone plume structure correlated with oriental fruit moth behaviour. Physiol Entomol 14:1–12

    Article  Google Scholar 

  • Becher PG, Bengtsson M, Hansson BS, Witzgall P (2010) Flying the fly: long-range flight behavior of Drosophila melanogaster to attractive odors. J Chem Ecol 36:599–607

    Article  PubMed  CAS  Google Scholar 

  • Bunchu N, Sukontason KL, Olson JK, Kurahashi H, Sukontason K (2008) Behavioral responses of Chrysomya megacephala to natural products. Parasitol Res 102:419–429

    Article  PubMed  Google Scholar 

  • Burkett DA, Butler JF (2005) Laboratory evaluation of colored light as an attractant for female Aedes aegypti, Aedes albopictus, Anopheles quadrimaculatus, and Culex nigripalpus. Florida Entomol 88:383–389

    Article  Google Scholar 

  • Cardé RT (1996) Odour plumes and odour-mediated flight in insects. In: Cardew G, Goode J (eds) Mosquito olfaction and olfactory-mediated mosquito–host interactions. Ciba Foundation Symposium 2000. John Wiley & Sons Ltd, New York, pp 54–70

    Google Scholar 

  • Chaudhury MF, Welch JB, Alvarez LA (2002) Responses of fertile and sterile screwworm (Diptera: Calliphoridae) flies to bovine blood inoculated with bacteria originating from screwworm-infested animal wounds. J Med Entomol 39:130–134

    Article  PubMed  CAS  Google Scholar 

  • Cooperband MF, McElfresh JS, Millar JG, Cardé RT (2008) Electroantennogram and behavioural responses of the malaria vector Anopheles gambiae to human-specific sweat components. J Insect Physiol 54:1184–1192

    Article  PubMed  CAS  Google Scholar 

  • Costantini C, Birkett MA, Gibson G, Ziesmann J, Sagnon NF, Mohammed HA, Coluzzi M, Pickett JA (2001) Electroantennogram and behavioural responses of the malaria vector Anopheles gambiae to human-specific sweat components. Med Vet Entomol 15:259–266

    Article  PubMed  CAS  Google Scholar 

  • Davidson MM, Butler RC, Teulon DAJ (2006) Starvation period and age affect the response of female Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) to odor and visual cues. J Insect Physiol 52:729–736

    Article  PubMed  CAS  Google Scholar 

  • Geier M, Bosch OJ, Boeckh J (1999) Influence of odour plume structure on upwind flight of mosquitoes towards hosts. J Exp Biol 202:1639–1648

    PubMed  Google Scholar 

  • Gibson G, Torr SJ (1999) Visual and olfactory responses of haematophagous Diptera to host stimuli. Med Vet Entomol 13:2–23

    Article  PubMed  CAS  Google Scholar 

  • Goldansaz SH, McNeil JN (2006) Effect of wind speed on the pheromone-mediated behavior of sexual morphs of the potato aphid, Macrosiphum euphorbiae (Thomas) under laboratory and field conditions. J Chem Ecol 32:1719–1729

    Article  PubMed  CAS  Google Scholar 

  • Gomes L, Gomes G, Casarin FE, da Silba IM, Sanches MR, Von Zuben CJ, Fowler HG (2007) Visual and olfactory factors interaction in resource-location by the blowfly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae), in natural conditions. Neotropical Entomol 36:633–639

    Article  Google Scholar 

  • Linhares AX, Avancini RP (1989) Ovarian development in the blowflies Chrysomya putoria and C. megacephala on natural diets. Med Vet Entomol 3:293–295

    Article  PubMed  CAS  Google Scholar 

  • Marchand D, McNeil JN (2000) Effects of wind speed and atmosphere pressure on mate searching behavior in the aphid parasitoid Aphidius nigripes (Hymenoptera: Aphidiidae). J Insect Behav 13:187–199

    Article  Google Scholar 

  • Ngoen-klan R, Moophayak K, Klong-klaew T, Irvine KN, Sukontason KL, Prangkio C, Somboon P, Sukontason K (2011) Do climatic and physical factors affect populations of the blow fly Chrysomya megacephala and house fly Musca domestica? Parasitol Res 109:1279–1292

    Article  PubMed  Google Scholar 

  • Okada K, Sakuma M (2009) An odor stimulator controlling odor temporal pattern applicable in insect olfaction study. Chem Senses 34:425–433

    Article  PubMed  Google Scholar 

  • Pipitgool V, Waree P, Sithithaworn P, Limviroj W (1998) Studies on biting density and biting cycle of Culex quinquefasciatus Say in Khon Kaen City, Thailand. Southeast Asian J Trop Med Public Health 29:333–336

    PubMed  CAS  Google Scholar 

  • Spradbery JP (1979) The reproductive status of Chrysomya species (Diptera: Calliphoridae) attracted to liver-baited blow fly traps in Papua New Guinea. J Aust Entomol Soc 18:57–61

    Article  Google Scholar 

  • Stensmyr MC, Giordano E, Balloi A, Angioy AM, Hasson BS (2003) Novel natural ligands for Drosophila olfactory receptor neurons. J Exp Biol 206:715–724

    Article  PubMed  CAS  Google Scholar 

  • Stoffolano JG Jr, Li MF, Sutton JA Jr, Yin CM (1995) Faeces feeding by adult Phormia regina (Diptera: Calliphoridae): impact on reproduction. Med Vet Entomol 9:388–392

    Article  PubMed  Google Scholar 

  • Sucharit S, Tumrasvin W, Vutikes S (1976) A survey on house flies in Bangkok and neighboring provinces. Southeast Asian J Trop Med Public Health 7:85–90

    Google Scholar 

  • Sukontason KL, Chaiwong T, Piangjai S, Upakut S, Moophayak K, Sukontason K (2008) Ommatidia of blow fly, house fly, and flesh fly: implication of their vision efficiency. Parasitol Res 103:123–131

    Article  PubMed  Google Scholar 

  • Turling TCJ, Davison AC, Tamò C (2004) A six-arm olfactometer permitting simultaneous observation of insect attraction and odour trapping. Physiol Entomol 29:45–55

    Article  Google Scholar 

  • Urech R, Green PE, Franke F, Mulder JC, Roberts C (1994) Behavioural responses of Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae) to olfactory stimuli: evaluation of an olfactometer. J Aust Entomol Soc 33:137–141

    Article  Google Scholar 

  • Vickers NJ, Christensen TA, Baker TC, Hildebrand JG (2001) Odour-plume dynamics influence the brain’s olfactory code. Nature 410:466–470

    Article  PubMed  CAS  Google Scholar 

  • Wall RL, Fisher P (2001) Visual and olfactory cue interaction in resource-location by the blowfly, Lucilia sericata. Physiol Entomol 26:212–218

    Article  Google Scholar 

  • Wall R, Cruickshank I, Smith KE, French NP, Holme AS (2002) Development and validation of a simulation model for blowfly strike of sheep. Med Vet Entomol 16:335–346

    Article  PubMed  CAS  Google Scholar 

  • Wang X, Zhong M, Liu Q, Aly SM, Wu C, Wen J (2013) Molecular characterization of the carbon dioxide receptor in the oriental latrine fly, Chrysomya megacephala (Diptera: Calliphoridae). Parasitol Res 112:2763–2771

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by grants from the Royal Golden Jubilee Ph.D. Program (PHD/0203/2548), Thailand Research Fund (RMU5080036), and Ananthamahidol Foundation. We thank the Faculty of Medicine and Chiang Mai University for facility.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand

    Kittikhun Moophayak, Kabkaew L. Sukontason & Kom Sukontason

  2. Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan

    Hiromu Kurahashi

  3. Department of Biology, Midwestern State University, Wichita Falls, TX, 76308, USA

    Roy C. Vogtsberger

Authors
  1. Kittikhun Moophayak
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  2. Kabkaew L. Sukontason
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  3. Hiromu Kurahashi
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  4. Roy C. Vogtsberger
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  5. Kom Sukontason
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Correspondence to Kom Sukontason.

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Moophayak, K., Sukontason, K.L., Kurahashi, H. et al. Evaluation of an I-box wind tunnel model for assessment of behavioral responses of blow flies. Parasitol Res 112, 3789–3798 (2013). https://doi.org/10.1007/s00436-013-3566-1

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  • DOI: https://doi.org/10.1007/s00436-013-3566-1

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