Abstract
Intestinal helmintic infection, continue to be a cause of major concern in several parts of the world, particularly in the developing nations. The use of plant extracts to control poultry helminths is increasing in different rearing systems. The anthelmintic activity of ginger and curcumin was studied on the nematode Ascaridia galli. In vitro and in vivo studies were allocated. Live parasites for in vitro studies were collected from the intestine of naturally infected chickens. Some living worms were incubated at 37 °C in media containing ginger at three concentration levels (25, 50, and 100 mg/ml), and others were incubated in media containing curcumin at the same concentration levels. Another living worm group was incubated in media containing albendazole at a dose of 7.5 mg/ml. The extracts' efficacy was exhibited in a concentration–time-dependent manner mainly at 100 mg/ml and after 48 h. The in vivo study takes place on experimentally infected chickens. Group of infected chickens was treated with ginger extract at dose of 100 mg, another group was treated with curcumin extract at dose of 100 mg, and a third group was treated with albendazole at dose of 7.5 mg. In vivo study of ginger and curcumin recorded lower mortality rates than the in vitro study. It is concluded that ginger and curcumin extracts have potential anthelmintic properties against A. galli. Ginger in all concentrations used exhibited a higher death rate observed than curcumin. Their wormicidal effect is concentration–time dependent.
Similar content being viewed by others
References
Abdel-Ghaffar F, Semmler M, Al-Rasheid KA, Strassen B, Fischer K, Aksu G, Klimpel S, Mehlhorn H (2011) The effects of different plant extracts on intestinal cestodes and on trematodes. Parasitol Res 108(4):979–984. doi:10.1007/s00436-010-2167-5
Abdelqader A, Gauly M, Wollny CB, Abo-Shehdada MN (2008) Prevalence and burden of gastrointestinal helminthes among local chickens in northern Jordan. Prev Vet Med 85:17–22
Abdelqader A, Qarallah B, Al-Ramamneh D, Daş G (2012) Anthelmintic effects of citrus peels ethanolic extracts against Ascaridia galli. Vet Parasitol 188(1–2):78–84. doi:10.1016/j.vetpar.2012.03.003
Adedapo A, Otesile AT, Soetan KO (2007) Assessment of the anthelmintic efficacy of an aqueous crude extract of Vernonia amygdalina. Pharm Biol 45:564–568
Ademola IO, Eloff JN (2011) Anthelminthic activity of acetone extract and fractions of Vernonia amygdalina against Haemonchus contortus eggs and larvae. Trop Anim Health Prod 43:521–527
Ahmad M, Nizami WA (1987) In vitro effects of mebendazole on the carbohydrate metabolism of Avitellina lahorea (Cestoda). J Helminthol 61:247–252
Allam G (2009) Immunomodulatory effects of curcumin treatment on murine Schistosomiasis mansoni. Immunobiology 214(8):712–727
Atjanasuppat K, Wongkham W, Meepowpan P, Kittakoop P, Sobhon P, Bartlett A, Whitfield PJ (2009) In vitro screening for anthelmintic and antitumour activity of ethnomedicinal plants from Thailand. J Ethnopharmacol 123:475–482
Banu MJ, Nellaiappan K, Dhandayuthapani S (1989) Lactate dehydrogenase from adult Setaria digitata (Nematoda: Onchocercidae). Vet Parasitol 32:311–323
Bashtar AR, Hassanein M, Abdel-Ghaffar F, Al-Rasheid K, Hassan S, Mehlhorn H, Al-Mahdi M, Morsy K, Al-Ghamdi A (2011) Studies on monieziasis of sheep I. Prevalence and antihelminthic effects of some plant extracts, a light and electron microscopic study. Parasitol Res 108(1):177–186. doi:10.1007/s00436-010-2060-2
Das G, Kaufmann F, Abel H, Gauly M (2010) Effect of extra dietary lysine in Ascaridia galli infected grower layers. Vet Parasitol 170:238–243
Datta A, Sukul NC (1987) Antifilarial effect of Zingiber officinale on Dirofilaria immitis. J Helminthol 61:268–270
Desrivot J, Waikedre J, Cabalion P, Herrenknecht C, Bories C, Hocquemiller R, Fournet A (2007) Antiparasitic activity of some New Caledonian medicinal plants. J Ethnopharmacol 112(1):7–12
Elango G, Rahuman AA (2011) Evaluation of medicinal plant extracts against ticks and fluke. Parasitol Res 108(3):513–519. doi:10.1007/s00436-010-2090-9
Ferraz ABF, Balbino JM, Zini CA, Ribeiro VL, Bordignon SA, von Poser G (2010) Acaricidal activity and chemical composition of the essential oil from three Piper species. Parasitol Res 107(1):243–248. doi:10.1007/s00436-010-1878-y
Goto C, Kasuya S, Koga K, Ohtomo H, Kagei N (1990) Lethal efficacy of extract from Zingiber officinale (traditional Chinese medicine) or [6]-shogaol and [6]-gingerol in Anisakis larvae in vitro. Parasitol Res 76:653–656
Hossain E, Chandra G, Nandy AP, Mandal SC, Gupta JK (2012) Anthelmintic effect of a methanol extract of leaves of Dregea volubilis on Paramphistomum explanatum. Parasitol Res 110(2):809–814. doi:10.1007/s00436-011-2558-2
Hussain H, Shukla OP, Ghatak S, Kaushal NA (1990) Enzymes of PEP-succinate pathway in Setaria cervi and effect of anthelmintic drugs. Indian J Exp Biol 28:871–875
Iqbal Z, Lateef M, Ashraf M, Jabbar A (2004) Anthelmintic activity of Artemisia brevifolia in sheep. J Ethnopharmacol 93:265–268
Iqbal Z, Lateef M, Akhtar MS, Ghayur MN, Gilani AH (2006) In vivo anthelmintic activity of ginger against gastrointestinal nematodes of sheep. J Ethnopharmacol 106:285–287
Kaufmann F, Das G, Sohnrey B, Gauly M (2011) Helminth infections in laying hens kept in organic free range systems in Germany. Livest Sci 141:182–187
Khalafalla RE, Müller U, Shahiduzzaman M, Dyachenko V, Desouky AY, Alber G, Daugschies A (2011) Effects of curcumin (diferuloylmethane) on Eimeria tenella sporozoites in vitro. Parasitol Res 108(4):879–886. doi:10.1007/s00436-010-2129-y
Klimpel S, Abdel-Ghaffar F, Al-Rasheid KA, Aksu G, Fischer K, Strassen B, Mehlhorn H (2011) The effects of different plant extracts on nematodes. Parasitol Res 108(4):1047–1054. doi:10.1007/s00436-010-2168-4
Lachumy SJT, Sasidharan S, Sumathy V, Zuraini Z (2010) Pharmacological activity, phytochemical analysis and toxicity of methanol extract of Etlingera elatior (torch ginger) flowers. Asian Pac J Trop Med 3:769–774
Lakhdar-Ghazal F, Blonski C, Willson M, Michels P, Perie J (2002) Glycolysis and proteases as targets for the design of new anti-trypanosome drugs. Curr Top Med Chem 2:439–456
Lin RJ, Chenc CY, Chunga LY, Yen CM (2010) Larvicidal activities of ginger (Zingiber officinale) against Angiostrongylus cantonensis. Acta Trop 115:69–76
Liu YT, Wang F, Wang GX, Han J, Wang Y, Wang YH (2010) In vivo anthelmintic activity of crude extracts of Radix angelicae pubescentis, Fructus bruceae, Caulis spatholobi, Semen aesculi, and Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Parasitol Res 106(5):1233–1239. doi:10.1007/s00436-010-1799-9
Magalhães LG, Machado CB, Morais ER, Moreira EB, Soares CS, da Silva SH, Da Silva Filho AA, Rodrigues V (2009) In vitro schistosomicidal activity of curcumin against Schistosoma mansoni adult worms. Parasitol Res 104(5):1197–1201. doi:10.1007/s00436-008-1311-y
Magalhães LG, Kapadia GJ, da Silva Tonuci LR, Caixeta SC, Parreira NA, Rodrigues V, Da Silva Filho AA (2010) In vitro schistosomicidal effects of some phloroglucinol derivatives from Dryopteris species against Schistosoma mansoni adult worms. Parasitol Res 106(2):395–401. doi:10.1007/s00436-009-1674-8
Mahady GB, Pendland SL, Yun GS, Lu ZZ, Stoia A (2003) Ginger (Zingiber officinale Roscoe) and the gingerols inhibit the growth of Cag A+ strains of Helicobacter pylori. Anticancer Res 23:3699–3702
Mahady GB, Pendland SL, Stoia A, Hamill FA, Fabricant D, Dietz BM, Chadwick LR (2005) In vitro susceptibility of Helicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders. Phytother Res 19:988–991
Maphosa V, Masika PJ (2012) The potential of Elephantorrhiza elephantina as an anthelminthic in goats. Parasitol Res 111(2):881–888. doi:10.1007/s00436-012-2914-x
Mehlhorn H, Al-Quraishy S, Al-Rasheid KA, Jatzlau A, Abdel-Ghaffar F (2011) Addition of a combination of onion (Allium cepa) and coconut (Cocos nucifera) to food of sheep stops gastrointestinal helminthic infections. Parasitol Res 108(4):1041–1046. doi:10.1007/s00436-010-2169-3
Mostafa OM, Eid RA, Adly MA (2011) Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice. Parasitol Res 109(2):395–403. doi:10.1007/s00436-011-2267-x
Nandakumar DN, Nagaraj VA, Vathsala PG, Rangarajan P, Padmanaban G (2006) Curcumin-artemisinin combination therapy for malaria. Antimicrob Agents Chemother 50(5):1859–1860
Nery PS, Nogueira FA, Oliveira NJ, Martins ER, Duarte ER (2012) Efficacy of extracts of immature mango on ovine gastrointestinal nematodes. Parasitol Res 111(6):2467–2471. doi:10.1007/s00436-012-3017-4
Nogueira FA, Fonseca LD, da Silva RB, de Paiva Ferreira AV, Nery PS, Geraseev LC, Duarte ER (2012) In vitro and in vivo efficacy of aqueous extract of Caryocar brasiliense Camb. to control gastrointestinal nematodes in sheep. Parasitol Res 111(1):325–330. doi:10.1007/s00436-012-2843-8
Nostro A, Cellini L, Di Bartolomeo S, Cannatelli MA, Di Campli E, Procopio F, Grande R, Marzio L, Alonzo V (2006) Effects of combining extracts (from propolis or Zingiber officinale) with clarithromycin on Helicobacter pylori. Phytother Res 20:187–190
Omar MS, Raoof AM, Al-Amari OM (1996) Onchocerca fasciata: enzyme histochemistry and tissue distribution of various dehydrogenases in the adult female worm. Parasitol Res 82:32–37
Pérez-Arriaga L, Mendoza-Magaña ML, Cortés-Zárate R, Corona-Rivera A, Bobadilla-Morales L, Troyo-Sanromán R, Ramírez-Herrera MA (2006) Cytotoxic effect of curcumin on Giardia lamblia trophozoites. Acta Trop 98(2):152–161
Rana AK, Misra-Bhattacharya S (2013) Current drug targets for helminthic diseases. Parasitol Res 112(5):1819–1831. doi:10.1007/s00436-013-3383-6
Rasmussen HB, Christensen SB, Kvist LP, Karazmi A (2000) A simple and efficient separation of the curcumins, the antiprotozoal constituents of Curcuma longa. Planta Med 66:396–398
Sanderson L, Bartlett A, Whitefield PJ (2002) In vitro and in vivo studies on the bioactivity of a ginger (Zingiber officinale) extract towards adult schistosomes and their egg production. J Helminthol 76:241–247
Schmahl G, Al-Rasheid KA, Abdel-Ghaffar F, Klimpel S, Mehlhorn H (2010) The efficacy of neem seed extracts (Tre-san®, MiteStop®) on a broad spectrum of pests and parasites. Parasitol Res 107(2):261–269. doi:10.1007/s00436-010-1915-x
Semmler M, Abdel-Ghaffar F, Al-Rasheid K, Mehlhorn H (2009) Nature helps: from research to products against blood-sucking arthropods. Parasitol Res 105(6):1483–1487. doi:10.1007/s00436-009-1634-3
Shahiduzzaman M, Dyachenko V, Khalafalla RE, Desouky AY, Daugschies A (2009) Effects of curcumin on Cryptosporidium parvum in vitro. Parasitol Res 105(4):1155–1161. doi:10.1007/s00436-009-1535-5
Sharma RK, Singh K, Saxena R, Saxena KK (1986) Effect of some anthelmintics on malate dehydrogenase activity and mortality in two avian nematodes Ascaridia galli and Heterakis gallinae. Angew Parasitol 27:175–180
Shyu LY, Chang HH, Hsu JD, Lin DP, Teng YH, Lee HH (2011) Curcumin alleviates eosinophilic meningitis through reduction of eosinophil count following albendazole treatment against Angiostrongylus cantonensis in mice. Parasitology 7:1–8
SPSS (2007) SPSS for Windows, version 16.0. Release 16.0.0. Chicago, IL
Squires JM, Foster J, Lindsay D, Caudell D, Zajac A (2010) Efficacy of an orange oil emulsion as an anthelmintic against Haemonchus contortus in gerbils (Meriones unguiculatus) and in sheep. Vet Parasitol 172:95–99
Srinivasan NG, Wariso BA, Kulkarni G, Rao GS, Harris BG (1988) Phosphofructokinase from Dirofilaria immitis. Stimulation of activity by phosphorylation with cyclic AMP-dependent protein kinase. J Biol Chem 263:3482–3485
Tangpu V, Temjenmongla, Yadav AK (2006) Anticestodal property of Strobilanthes discolor: an experimental study in Hymenolepis diminuta—rat model. J Ethnopharmacol 105:459–463
Wu ZF, Zhu B, Wang Y, Lu C, Wang GX (2011) In vivo evaluation of anthelmintic potential of medicinal plant extracts against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Parasitol Res 108(6):1557–1563. doi:10.1007/s00436-010-2211-5
Yazwinski TA, Chapman HD, Davis RB, Letonja T, Pote L, Maes L, Vercruysse J, Jacobs DE (2003) World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines for evaluating the effectiveness of anthelmintics in chickens and turkeys. Vet Parasitol 116:159–173
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bazh, E.K.A., El-Bahy, N.M. In vitro and in vivo screening of anthelmintic activity of ginger and curcumin on Ascaridia galli . Parasitol Res 112, 3679–3686 (2013). https://doi.org/10.1007/s00436-013-3541-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-013-3541-x