Abstract
Little information is available on the epidemiology of Cryptosporidium in pigs in central Vietnam. The aims of this study were to investigate the prevalence and to characterize the genotype distribution of Cryptosporidium isolates in pigs in this region. A total of 193 pig fecal samples were screened for the presence of Cryptosporidium oocysts using the modified Ziehl–Neelsen staining method, and 28 (overall prevalence 14.5 %) were identified as positive by microscopic observation. Positive samples were further analyzed by polymerase chain reaction amplification and sequencing. Genetic identification based on the 18S ribosomal RNA and 70 kDa heat shock protein genes revealed that pigs in Vietnam are infected with two species/genotypes (Cryptosporidium suis and Cryptosporidium pig genotype II). This study is the first molecular characterization of Cryptosporidium in pigs in Vietnam. The presence of these host-adapted species/genotypes suggests that pigs may not pose a significant public health risk in this area. More extensive studies are necessary to ascertain the zoonotic potential of Cryptosporidium in porcine hosts in Vietnam.
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Acknowledgments
This study was supported by the Japan Society for the Promotion of Science (JSPS) under the Ronpaku Fellowship (no. VAST-10936) for Sam Thi Nguyen. The authors would like to thank colleagues in the Laboratory of Sustainable Environmental Biology, Tohoku University and at the Central Vietnam Veterinary Institute for their supports.
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Nguyen, S.T., Fukuda, Y., Tada, C. et al. Molecular characterization of Cryptosporidium in pigs in central Vietnam. Parasitol Res 112, 187–192 (2013). https://doi.org/10.1007/s00436-012-3124-2
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DOI: https://doi.org/10.1007/s00436-012-3124-2