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Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

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Abstract

A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial small subunit ribosomal RNA (SSU rRNA) and GP60 partial genes and polymerase chain reaction restriction fragment length polymorphism of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and two sows (10%). Twenty-one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and Cryptosporidium suis in finishers were found. Both sows were infected with the Cryptosporidium parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.

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Acknowledgments

This work was supported by the grant of the Ministry of Education, Youth and Sports of the Czech Republic (MSM 6007665806), the Grant Agency of the Czech Republic (project no. 523/07/P117), and research project of the Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic (Z60220518).

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Authors and Affiliations

  1. Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Martin Kváč, Bohumil Sak & Dana Květoňová

  2. Faculty of Agriculture, University of South Bohemia in České Budějovice, Studentská 13, 370 05, České Budějovice, Czech Republic

    Martin Kváč, Dagmar Hanzlíková & Jiřina Kotilová

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  1. Martin Kváč
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  2. Bohumil Sak
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  3. Dagmar Hanzlíková
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  4. Jiřina Kotilová
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  5. Dana Květoňová
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Correspondence to Bohumil Sak.

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Kváč, M., Sak, B., Hanzlíková, D. et al. Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic. Parasitol Res 104, 425–428 (2009). https://doi.org/10.1007/s00436-008-1215-x

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  • DOI: https://doi.org/10.1007/s00436-008-1215-x

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