Abstract
The protozoan parasite Haplosporidium nelsoni (MSX) was identified in Japanese scallops Patinopecten yessoensis (Jay, 1857) from Dalian along the northern coast of the Yellow Sea, China by histopathologic examination, polymerase chain reaction (PCR) amplification, and in situ hybridization (ISH) assay. H. nelsoni plasmodia-like structures were identified in the digestive glands of scallops by histologic examination, but no parasite spores were observed. PCR using the Hap-F2, R2 primer pair produced a sequence with 100% homology with the corresponding small subunit rDNA region of H. nelsoni. An ISH assay using the oligonucleotide probe MSX1347 produced a positive reaction with the Japanese scallop parasite. This is the first report of H. nelsoni in P. yessoensis in China.
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Acknowledgments
We thank S.F. Sui et al. for their assistance in the collection of Japanese scallop samples. We also acknowledge the contribution of the reviewers to improving the manuscript. This work is supported by the National Natural Science Foundation of China under contract no. 30470275 and the National Special Grant of China under contract no. 908-01-ZH3.
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Wang, Z., Lu, X. & Liang, Y. Haplosporidium nelsoni (MSX) in Japanese scallops Patinopecten yessoensis (Jay, 1857) from Dalian along the northern coast of the Yellow Sea, China. Parasitol Res 110, 1445–1451 (2012). https://doi.org/10.1007/s00436-011-2647-2
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DOI: https://doi.org/10.1007/s00436-011-2647-2