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Biochemical evidence of efficacy of potash alum for the control of dengue vector Aedes aegypti (Linnaeus)

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Abstract

Aedes aegypti is the primary vector of dengue, yellow fever and chikungunya in India and other South East Asian countries, and novel insecticides for vector control are urgently needed. In the present investigation, efficacy of potash alum, a traditionally known double salt in Indian and Chinese medicine system, was tested against the larvae of dengue vector, A. aegypti. LC50, LC90 and LC99 values were recorded for various instar larvae where I instar larvae were found to be the most susceptible and IV instar larvae as the least susceptible one. The LC50 values of crude and standard potash alum of various instar larvae ranged between 15.29 and 48.53 ppm and 20.50–65.10 ppm, respectively. Biochemical changes were also evidenced in IV instar A. aegypti larvae following a sublethal exposure for 24 h in the levels of various nutrient reserves and primary metabolites such as sugar, glycogen, lipids and proteins suggesting possible mode of action responsible for larval mortality. Sugar and glycogen concentrations were measured as 24.6 and 10.67 μg per five larvae in controls which were significantly (p < 0.05) reduced by 32.11–93.98% and 39.26–94.47%, respectively, in larvae treated with crude alum. In controls, protein and lipid content were recorded as 210.74 and 94.71 μg per five larvae which dropped up to 26.53% and 25.5%, respectively, in larvae following treatment with crude alum. Moreover, drastic changes were also recorded for DNA content with 25.39–44.17% decrease in crude alum-treated larvae. It is evident from these results that potash alum, a fairly cheaper and readily available ecofriendly compound could be recommended as a potential chemical larvicide against dengue vector at mosquito breeding sites in the vicinity of human dwellings.

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Acknowledgements

We are thankful to the University Grants Commission and the Department of Science and Technology, New Delhi, India for the financial assistance and gratefully acknowledge Prof. V.G. Das, Director, Dayalbagh Educational Institute, Dayalbagh, Agra for providing the necessary facilities.

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  1. Department of Zoology, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra, 282110, India

    Shabad Preet & A. Sneha

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  1. Shabad Preet
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Correspondence to Shabad Preet.

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Preet, S., Sneha, A. Biochemical evidence of efficacy of potash alum for the control of dengue vector Aedes aegypti (Linnaeus). Parasitol Res 108, 1533–1539 (2011). https://doi.org/10.1007/s00436-010-2210-6

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