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Genetic variability of Blastocystis sp. isolates obtained from cancer and HIV/AIDS patients

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Abstract

This represents the first study to determine the genetic diversity of Blastocystis sp. among cancer and HIV/AIDS patients. Forty Blastocystis sp. isolates obtained from 20 cancer and 20 HIV/AIDS patients were genotyped by PCR using seven pairs of known sequenced-tagged site primers. Out of the 40 isolates, 38 were identified as one of the known genotypes and two isolates were negative with all the STS primers. Blastocystis sp. subtype 3 which is reported to be associated with disease was found to be predominant among the study subjects.

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References

  • Abe N (2004) Molecular and phylogenetic analysis of Blastocystis isolates from various hosts. Vet Parasitol 120:235–242

    Article  CAS  PubMed  Google Scholar 

  • Albrecht H, Stellbrink HJ, Koperski K, Greten H (1995) Blastocystis hominis in human immunodeficiency virus-related diarrhea. Scand J Gastroenterol 30:909–914

    Article  CAS  PubMed  Google Scholar 

  • Böhm-Gloning B, Knobloch J, Walderich B (1997) Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR amplified 16S-like rDNA. Trop Med Int Health 2:771–778

    Article  PubMed  Google Scholar 

  • Brites C, Barberino MG, Bastos MA, Sampaio Sa M, Silva N (1997) Blastocystis hominis as a potential cause of diarrhea in AIDS patients: a report of six cases in Bahia, Brazil. Braz J Infect Dis 1:91–94

    PubMed  Google Scholar 

  • Cirioni O, Giacometti A, Drenaggi D, Ancarani F, Scalise G (1999) Prevalence and clinical relevance of Blastocystis hominis in diverse patient cohorts. Eur J Epidemiol 15:389–393

    Article  CAS  PubMed  Google Scholar 

  • Clark CG (1997) Extensive genetic diversity in Blastocystis hominis. Mol Biochem Parasitol 87:79–83

    Article  CAS  PubMed  Google Scholar 

  • Dogruman-Al F, Dagci H, Yoshikawa H, Kurt O, Demirel M (2008) A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis. Parasitol Res 103:685–689

    Article  PubMed  Google Scholar 

  • Domínguez-Márquez MV, Guna R, Muñoz C, Gómez-Muñoz MT, Borrás R (2009) High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain). Parasitol Res. doi:10.1007/s00436-009-1485-y

  • Germani Y, Minssart P, Vohito M, Yassibanda S, Glaziou P, Hocquet D, Berthelemy P, Morvan J (1998) Etiologies of acute, persistent, and dysenteric diarrhea in adults in Bangui, Central African Republic, in relation to human immunodeficiency virus serostatus. Am J Trop Med Hyg 59:1008–1014

    CAS  PubMed  Google Scholar 

  • Ghosh K, Ayyaril M, Nirmala V (1998) Acute GVHD involving the gastrointestinal tract and infestation with Blastocystis hominis in a patient with chronic myeloid leukaemia following allogeneic bone marrow transplantation. Bone Marrow Transplant 22:1115–1117

    Article  CAS  PubMed  Google Scholar 

  • Jones WR (1946) The experimental infection of rats with Entamoeba histolytica. Ann Trop Med Parasitol 40:130

    Google Scholar 

  • Jones MS, Whipps CM, Ganac RD, Hudson NR, Boorom K (2009) Association of Blastocystis subtype 3 and 1 with patients from an Oregon community presenting with chronic gastrointestinal illness. Parasitol Res 104:341–345

    Article  PubMed  Google Scholar 

  • Junod C (1995) Blastocystis hominis: a common commensal in the colon. Study of prevalence in different populations of Paris. Presse Med 24:1684–1688

    CAS  PubMed  Google Scholar 

  • Katsarou-Katsari A, Vassalos CM, Tzanetou K, Spanakos G, Papadopoulou C, Vakalis N (2008) Acute urticaria associated with amoeboid forms of Blastocystis sp. subtype 3. Acta Derm Venereol 88:80–81

    Article  PubMed  Google Scholar 

  • Koltas S, Ozcan K, Tannverdi S, Paydas S, Baslamish F (1999) The prevalence of Blastocystis hominis in immunosuppressed patients. Ann. Med. Sci 8:117–119

    Google Scholar 

  • Kurniawan A, Karyadi T, Dwintasari SW, Sari IP, Yunihastuti E, Djauzi S, Smith HV (2009) Intestinal parasitic infections in HIV/AIDS patients presenting with diarrhoea in Jakarta, Indonesia. Trans R Soc Trop Med Hyg. doi:10.1016/j.trstmh.2009.02.017

  • Li LH, Zhang XP, Lv S, Zhang L, Yoshikawa H, Wu Z, Steinmann P, Utzinger J, Tong XM, Chen SH, Zhou XN (2007) Cross-sectional surveys and subtype classification of human Blastocystis isolates from four epidemiological settings in China. Parasitol Res 102:83–90

    Article  PubMed  Google Scholar 

  • Noël C, Dufernez F, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Ho LC, Singh M, Wintjens R, Sogin ML, Capron M, Pierce R, Zenner L, Viscogliosi E (2005) Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J Clin Microbiol 43:348–355

    Article  PubMed  Google Scholar 

  • Ok UZ, Cirit M, Uner A, Ok E, Akcicek F, Basci A, Ozcel MA (1997) Cryptosporidiosis and blastocystosis in renal transplant recipients. Nephron 75:171–174

    Article  CAS  PubMed  Google Scholar 

  • Ozyurt M, Kurt O, Mølbak K, Nielsen HV, Haznedaroglu T, Stensvold CR (2008) Molecular epidemiology of Blastocystis infections in Turkey. Parasitol Int 57:300–306

    Article  PubMed  Google Scholar 

  • Prasad KN, Nag VL, Dhole TN, Ayyagari A (2000) Identification of enteric pathogens in HIV-positive patients with diarrhoea in northern India. J Health Popul Nutr 18:23–26

    CAS  PubMed  Google Scholar 

  • Rao K, Sekar U, Iraivan KT, Abraham G, Soundararajan P (2003) Blastocystis hominis—an emerging cause of diarrhoea in renal transplant recipients. J Assoc Physicians India 51:719–721

    CAS  PubMed  Google Scholar 

  • Rivera WL (2008) Phylogenetic analysis of Blastocystis isolates from animal and human hosts in the Philippines. Vet Parasitol 156:178–182

    Article  CAS  PubMed  Google Scholar 

  • Souppart L, Sanciu G, Cian A, Wawrzyniak I, Delbac F, Capron M, Dei-Cas E, Boorom K, Delhaes L, Viscogliosi E (2009) Molecular epidemiology of human Blastocystis isolates in France. Parasitol Res. 105:413–421

    Google Scholar 

  • Stensvold CR, Suresh GK, Tan KS, Thompson RC, Traub RJ, Viscogliosi E, Yoshikawa H, Clark CG (2007) Terminology for Blastocystis subtypes—a consensus. Trends Parasitol 23:93–96

    Article  PubMed  Google Scholar 

  • Stenzel DJ, Boreham PFL (1996) Blastocystis hominis revisited. Clin Microbiol Rev 9:563–584

    CAS  PubMed  Google Scholar 

  • Tan KSW, Singh M, Yap EH (2002) Recent advances in Blastocystis hominis research: hot spot in terra incognita. Int J Parasitol 32:789–804

    Article  CAS  PubMed  Google Scholar 

  • Tan TC, Suresh KG, Smith HV (2008) Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. Parasitol Res 104:85–93

    Article  CAS  PubMed  Google Scholar 

  • Taşova Y, Sahin B, Koltaş S, Paydaş S (2000) Clinical significance and frequency of Blastocystis hominis in Turkish patients with hematological malignancy. Acta Med Okayama 54:133–136

    PubMed  Google Scholar 

  • Wong KH, Ng GC, Lin RT, Yoshikawa H, Taylor MB, Tan KS (2008) Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Parasitol Res 102:663–670

    Article  PubMed  Google Scholar 

  • Yan Y, Su S, Lai R, Liao H, Ye J, Li X, Luo X, Chen G (2006) Genetic variability of Blastocystis hominis isolates in China. Parasitol Res 99:597–601

    Article  PubMed  Google Scholar 

  • Yoshikawa H, Nagono I, Yap EH, Singh M, Takahashi Y (1996) DNA polymorphism revealed by arbitrary primers polymerase chain reaction among Blastocystis strains isolated from humans, a chicken and a reptile. J Eukaryot Microbiol 43:127–130

    Article  CAS  PubMed  Google Scholar 

  • Yoshikawa H, Wu Z, Kimata I, Iseki M, Ali IK, Hossain MB, Zaman V, Haque R, Takahashi Y (2004) Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries. Parasitol Res 93:22–29

    Google Scholar 

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

    T. C. Tan, S. C. Ong & K. G. Suresh

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  1. T. C. Tan
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  2. S. C. Ong
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  3. K. G. Suresh
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Correspondence to K. G. Suresh.

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Tan, T.C., Ong, S.C. & Suresh, K.G. Genetic variability of Blastocystis sp. isolates obtained from cancer and HIV/AIDS patients. Parasitol Res 105, 1283–1286 (2009). https://doi.org/10.1007/s00436-009-1551-5

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  • DOI: https://doi.org/10.1007/s00436-009-1551-5

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