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L3L4ES antigen and secretagogues induce histamine release from porcine peripheral blood basophils after Ascaris suum infection

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Abstract

The aim of this paper was to investigate the role of porcine basophils in protective immunity. Experimental pigs were infected with 103 Ascaris suum eggs daily for 21 days. Control pigs were maintained helminth-free. Circulating porcine basophils were isolated from the anticoagulated whole blood of A. suum-infected and noninfected pigs by dextran (4.5%) sedimentation of erythrocytes or by the centrifugation of dextran-isolated leukocytes through discontinuous Percoll gradients. Results showed that 2.2% of the isolated leukocytes, stained with May-Grunwald Giemsa, were basophils. Each basophil from infected pigs contained 1.30 × 10−2 to 1.20 × 10−1 pg of histamine. Peripheral blood basophils (PBBs) from infected swine released 49% specific histamine when induced with A. suum-derived antigen (L3L4ES), 55% with anti-immunoglobulin G, and 62% with calcium ionophore A23l87. During A. suum infection, the number of isolated basophils and histamine levels peaked at 14 to 21 days postinfection and then showed a significant decrease. Percent-specific histamine released from PBBs by infected swine was significantly greater than that released by control pigs. The L3L4ES antigen and secretagogues effectively induced specific/nonspecific histamine release from PBBs and should facilitate future investigations of porcine basophils.

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Acknowledgments

Support for this work was generated by a student fellowship from the Graduate School of Arts and Sciences, Howard University (Washington, DC 20059) and the U.S. Department of Agriculture, Parasitology Institute (Beltsville, MD) to P. I. Uston.

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Authors and Affiliations

  1. Department of Biology, Howard University, 415 College Street, NW, Room 126, Washington, DC, 20059, USA

    P. I. Uston, C. M. Lee & F. R. Ampy

  2. United States Department of Agriculture, Nutrient Requirements and Functions Laboratory, Agricultural Research Services, Building 307, BARC-E, Beltsville, MD, 20705, USA

    J. F. Jr. Urban

  3. Comprehensive Sciences, Howard University, Locke Hall, Washington, DC, 20059, USA

    M. Ashraf

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  1. P. I. Uston
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  2. J. F. Jr. Urban
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  3. M. Ashraf
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  4. C. M. Lee
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  5. F. R. Ampy
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Correspondence to C. M. Lee.

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Uston, P.I., Jr. Urban, J.F., Ashraf, M. et al. L3L4ES antigen and secretagogues induce histamine release from porcine peripheral blood basophils after Ascaris suum infection. Parasitol Res 100, 603–611 (2007). https://doi.org/10.1007/s00436-006-0362-1

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