Abstract
Entamoeba histolytica produces monocyte locomotion inhibitory factor (MLIF), a pentapeptide with in vitro and in vivo anti-inflammatory properties. MLIF may interfere with leukocyte migration, disturbing the balance of pro- and anti-inflammatory cytokines secreted by CD4+ T lymphocytes. We evaluated the effect of MLIF on expression of pro- and anti-inflammatory cytokines in human CD4+ T lymphocytes. Regulatory cytokines [interleukin-1 beta (IL-1β), IL-2, interferon gamma (IFN-γ), IL-5, IL-6, and IL-10] were studied by enzyme-linked immunosorbent assay method in CD4+-cell supernatant fluids. Proinflammatory cytokines were produced per se by MLIF (IL-1β, IL-2, and IFN-γ) and also anti-inflammatory cytokines (IL-5, IL-6, and IL-10) with 1-phorbol-12 myristate-13 acetate + MLIF; the IL-1β, IFN-γ, IL-5 and IL-6 production was inhibited but not that of IL-10 which disclosed increase in its expression. MLIF disturbs the pro- and anti-inflammatory balance, and it induces inhibition of IL-1β (principal proinflammatory cytokine) and increases IL-10 (prototype of an anti-inflammatory cytokine).
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Acknowledgements
This work was supported by a grant from the Consejo Nacional de Ciencia y Tecnología (CONACYT), México (no. 38104-M). Sara Rojas-Dotor, Programa de Posgrado en Ciencias Biologícas UAM-X/I, is the recipient of a fellowship from CONACYT and IMSS México City, México. The authors are grateful to laboratory technician José de la Luz Romero-Preciado for the blood donation from the Mexican Institute of Social Security’s (IMSS) Centro Médico Nacional Siglo XXI (CMN-SXXI) Blood Bank in Mexico City.
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Rojas-Dotor, S., Rico, G., Pérez, J. et al. Cytokine expression in CD4+ cells exposed to the monocyte locomotion inhibitory factor produced by Entamoeba histolytica . Parasitol Res 98, 493–495 (2006). https://doi.org/10.1007/s00436-005-0090-y
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DOI: https://doi.org/10.1007/s00436-005-0090-y