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Differential display analysis of gene expression in two immunologically distinct strains of Eimeria maxima

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Abstract

Gene expression during sporulation and sporozoite excystation of two strains of Eimeria maxima was analyzed using the mRNA differential display technique. The two strains, the Guelph strain (GS) and a single sporocyst-derived strain (M6) from Florida, have been shown to be immunologically distinct. We isolated and cloned a 453-bp complimentary DNA (cDNA) fragment (GS-453) found only in GS sporozoites. In GS, this mRNA begins to be expressed during the earliest stages of oocyst sporulation and is continuously expressed up to and including in the excysted sporozoite. In all Northern blots, digoxigenin (DIG)-labeled GS-453 probe recognized an mRNA of approximately 1.6 kb from GS but not from RNA of M6. Southern blots using various endonucleases and probed with DIG-labeled GS-453 demonstrated that the genomes of both strains contained sufficiently similar sequences to permit hybridization with the probe, but the pattern of hybridization differed between the two strains. Extensive searches of the GenBank, European Molecular Biology Laboratory, and various apicomplexan expressed sequence tag databases using the DNA or inferred amino acid sequences of GS-453 cDNA clone did not identify similarity to any existing sequences.

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Acknowledgements

The local Animal Care Committee under the Canadian Council on Animal Care (CCAC) guidelines approved all animal experiments. We wish to thank Julie Cobean and Uyen Ky for skilled technical assistance. This work was supported by research grants from the Natural Sciences and Engineering Research Council of Canada and the Ontario Ministry of Agriculture and Food to J.R.B. and M.A.F.

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Author notes

  1. S. Lee

    Present address: Laboratory Services Division, University of Guelph, Guelph, N1H 8J7, Ontario, Canada

Authors and Affiliations

  1. Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, N1G 2W1, Ontario, Canada

    S. C. Basak, S. Lee, J. R. Barta & M. A. Fernando

Authors
  1. S. C. Basak
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  2. S. Lee
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  3. J. R. Barta
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  4. M. A. Fernando
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Correspondence to M. A. Fernando.

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Basak, S.C., Lee, S., Barta, J.R. et al. Differential display analysis of gene expression in two immunologically distinct strains of Eimeria maxima . Parasitol Res 99, 28–36 (2006). https://doi.org/10.1007/s00436-005-0087-6

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  • DOI: https://doi.org/10.1007/s00436-005-0087-6

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