Abstract
A myxosporean of the genus Chloromyxum sp., which sporulates in the kidney tubules, was detected in Atlantic salmon, Salmo salar L., and brown trout, Salmo trutta L., from three sites in Scotland. A comparison with other reports of Chloromyxum spores in the kidney of European salmonids suggests that they all refer to the same species, Chloromyxum schurovi [Shul’man BS, Ieshko EP (2003). Parazitologia 37:246–247]. Apart from Chloromyxum majori [Yasutake WT, Wood EM (1957). J Parasitol 43:633–637] which has a different geographical and host range, as well as tissue localisation, this is the only representative of the genus in the kidney of salmonids described so far. This study provides a re-description of C. schurovi including its myxosporean and actinosporean stage, i.e. Neoactinomyxum eiseniellae [Ormieres R, Frezil JL (1969). Protistologica 5:137–144], which was identified by 18S rDNA sequence identity. Results of phylogenetic analysis of the 18S rDNA sequence of C. schurovi and all other Chloromyxum species with published sequences showed that C. schurovi clusters with representatives of other myxozoan genera which inhabit the urinary system, whereas the other Chloromyxum species form a more basic clade together with another gall bladder parasite.
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Andree KB, Szekely C, Molnar K, Gresoviac SJ, Hedrick RP (1999) Relationships among members of the genus Myxobolus (Myxozoa: Bilvalvidae) based on small subunit ribosomal DNA sequences. J Parasitol 85:68–74
Eszterbauer E (2004) Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Dis Aquat Organ 58:35–40
Feist SW, Peeler EJ, Gardiner R, Smith E, Longshaw M (2002) Proliferative kidney disease and renal myxosporidiosis in juvenile salmonids from rivers in England and Wales. J Fish Dis 25:451–458
Felsenstein J (1993) Phylogeny inference package (PHYLIP). University of Washington, Seattle
Fiala I, Dyková I (2004) The phylogeny of marine and freshwater species of the genus Chloromyxum Mingazzini, 1890 (Myxosporea, Bivalvulida) based on small subunit ribosomal RNA gene sequences. Folia Parasitol 51:211–214
Holst JC, Nilsen F, Hodneland K, Nylund A (1993) Observations of the biology and parasites of postsmolt Atlantic salmon, Salmo salar, from the Norwegian Sea. J Fish Biol 42:962–966
Holzer AS, Sommerville C, Wootten R (2004) Molecular relationships and phylogeny in a community of myxosporeans and actinosporeans based on their 18S rDNA sequences. Int J Parasitol 34:1099–1111
Kent ML, Margolis L, Corliss JO (1994) The demise of a class of protists—taxonomic and nomenclatural revisions proposed for the protist phylum Myxozoa Grasse, 1970. Can J Zool 72:932–937
Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156
Lom J, Dykova I (1993) Scanning electron microscopic revision of common species of the genus Chloromyxum (Myxozoa, Myxosporea) infecting European freshwater fishes. Folia Parasitol 40:161–174
Lom J, McGeorge J, Feist SW, Morris D, Adams A (1997) Guidelines for the uniform characterisation of the actinosporean stages of parasites of the phylum Myxozoa. Dis Aquat Organ 30:1–9
Marques A (1984) Contribution a la connaissance des Actinomyxidies: ultrastructure, cycle biologique, systematique. Ph.D. thesis, Universite des Sciences et Techniques de Languedoc, Monpellier
Molnar K, El-Mansy A, Szekely C, Baska F (1999) Experimental identification of the actinosporean stage of Sphaerospora renicola Dyková & Lom 1982 (Myxosporea: Sphaerosporidae) in oligochaete alternate hosts. J Fish Dis 22:143–153
Ormieres R, Frezil JL (1969) Aurantiactinomyxon eiseniellae n. sp., actinomyxidie parasite de l’oligochete Eiseniella tetraedra Savinj (Oligochaeta, Lumbricidae). Protistologica 5:137–144
Sedlaczek J (1991) Chloromyxum sp. (Myxosporidia) in der Niere von Salmo trutta f. fario (Osteichthyes). Angew Parasitol 32:137–142
Shul’man BS, Ieshko EP (2003) Chloromyxum schurovi sp. n.—a new myxosporidian species (Myxosporea: Sphaerosporidae) from salmonid fishes (Salmonidae). Parazitologia 37:246–247
Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W—improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Yasutake WT, Wood EM (1957) Some myxosporidia found in Pacific Northwest salmonids. J Parasitol 43:633–637
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This research was funded by a DOC studentship to A.H. from the Austrian Academy of Sciences.
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Holzer, A.S., Sommerville, C. & Wootten, R. Molecular identity, phylogeny and life cycle of Chloromyxum schurovi Shul’man & Ieshko 2003. Parasitol Res 99, 90–96 (2006). https://doi.org/10.1007/s00436-005-0057-z
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DOI: https://doi.org/10.1007/s00436-005-0057-z