Abstract
Considering the snake venoms’ pharmacological properties and chemotherapeutic potential as well as the need for new alternatives for Giardia infection treatment, the present study was carried out aiming to evaluate the in vitro effects of crude Crotalus durissus terrificus and Bothrops jararaca venoms on the growth and adherence of Giardia duodenalis trophozoites. Trophozoites (106) were exposed to serial twofold dilutions of C. durissus terrificus and B. jararaca venoms that ranged from 3.125 to 200 μg/ml and from 5 to 320 μg/ml, respectively. The two venoms inhibited the growth of trophozoites, and the level of inhibition varied according to the assayed concentrations and incubation times. The highest reduction of parasite growth was observed with increasing concentrations of the venoms. No effect on parasite adherence was observed. Light microscope observations revealed changes of the pear-shape aspect of the cell and reduction of flagellar beating frequency in the great part of the trophozoites. This is the first attempt to evaluate the in vitro effects of snake venoms on Giardia trophozoites. The findings stress the need for more investigations and prompt us to perform further studies to identify active principles with antigiardial effect.
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References
Barat LM, Bloland PB (1997) Drug resistance among malaria and other parasites. Infect Dis Clin North Am 11:969–987
Bendesky A, Menédez D, Ostrosky-Wegman P (2002) Is metronidazole carcinogenic? Mutat Res 511:133–144
Bercovici D, Chudziniski AM, Dias WO, Esteves MI, Hiraichi E, Oishi NY, Picarelli ZP, Rocha MC da, Ueda CMPM, Yamanouye N, Raw I (1987) A systematic fractionation of Crotalus durissus terrificus venom. Mem Inst Butantan 49:69–78
Chin JB, Scheinin DM, Rauth AM (1978) Screening for mutagenicity of nitro-group containing hypoxic cell radiosensitizers using Salmonella typhimurium strains TA100 and TA98. Mutat Res 58:1–10
De Meo M, Vanelle P, Bernardini F, Laget M, Maldonado J, Jentzer O, Crozet MP, Dumenil G (1992) Evaluation of the mutagenic and genotoxic activities of 48 nitroimidazoles and related imidazole derivates by the Ames test and the SOS chromotest. Environ Mol Mutagen 19:167–181
Edlind TD, Hang TI (1990) Activity of the anthelmintic benzimidazoles against Giardia lamblia in vitro. J Infect Dis 162:1408–1411
Fernandez-Gomes R, Zerrouk H, Sebti F, Loyens M, Benslimane A, Ouaissi MA (1994) Growth inhibition of Trypanosoma cruzi and Leishmania donovani infantum by different snake venoms: preliminary identification of proteins from Cerastes cerastes venom which interact with the parasites. Toxicon 32:875–882
Freitas SF, Shinohara L, Sforcin JM, Guimarães S (in press) In vitro effects of propolis on Giardia duodenalis trophozoites. Phytomedicine
Gonçalves AR, Soares MJ, de Souza W, Da Matta RA, Alves EW (2002) Ultrastructural alterations and growth inhibition of Trypanosoma cruzi and Leishmania major induced by Bothrops jararaca venom. Parasitol Res 88:598–602
Harris JC, Plummer S, Turner ME, Lloyd D (2000) The microaerophilic flagellate Giardia intestinalis: Allium sativum (garlic) is an effective antigiardial. Microbiol 146:3119–3127
Hayashi MAF, Camargo ACM (2005) The bradykinin-potentiating peptides from venom gland and brain of Bothrops jararaca contain highly site specific inhibitors of the somatic angiotensin-converting enzyme. Toxicon 45:1163–1170
Keister DB (1983) Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 77:487–488
Jacques C, Loiseau PPM, Goudey-Perriere F (1998) In vitro antiprotozoal activity of snake venoms on kinetoplastidae. Toxicon 36:1739
Lemée V, Zaharia I, Nevez G, Rabodonirina M, Brausser P, Ballet JJ, Favennec L (2000) Metronidazole and albendazole susceptibility of eleven French human isolates of Giardia duodenalis. J Antimicrob Chemother 46:819–821
Lipps BV (1999) Novel snake venom proteins cytolytic to cancer cells in vitro and in vivo systems. J Venom Anim Toxins 5:173–183
McAllister TA, Annett CB, Cockwill CL, Olson ME, Wang Y, Cheeke PR (2001) Studies on the use of Yucca schidigera to control giardiosis. Vet Parasitol 97:85–99
Saguchi K, Hagiwara-Saguchi Y, Murayama N, Ohi H, Fujita Y, Camargo ACM, Serrano SMT, Higuchi S (2005) Molecular cloning of serine proteinases from Bothrops jararaca venom gland. Toxicon 46:72–83
Silva RJ, Fecchio D, Barraviera B (1997) Effec of Crotalus durissus terrificus venom on the Ehrlich ascites tumor. J Venom Anim Toxins 3:324–341
Silva RJ, Silva MG, Vilela CV, Fecchio D (2002) Antitumor effect of Botrhops jararaca venom. Mediators Inflamm 11:99–104
Sousa MC, Poiares-da-Silva J (2001) Cytotoxic effects of ciprofloxacin in Giardia lamblia trophozoites. Toxicol In Vitro 15:297–301
Stilles BG, Sexton FW, Weinstein SA (1991) Antibacterial effects of different snake venoms: purification and characterization of antibacterial proteins from Pseudechis australis (Australian king brown or mulga snake) venom. Toxicon 29:1129–1141
Tempone AR, Andrade HF, Spencer PJ, Lourenço CO, Rogero JR, Nascimento N (2001) Bothrops moojeni venom kills Leishmania spp. with hydrogen peroxide generated by its l-amino acid oxidase. Biochem Biophys Res Commun 280:620–624
Thompson RC (2000) Giardiasis as a re-emerging infectious disease and its zoonotic potential. Int J Parasitol 30:1259–1267
Upcroft P, Upcroft JA (2001) Drug targets and mechanisms of resistance in the anaerobic protozoa. Clin Microbiol Rev 14:150–164
Zar JH (1996) Biostatistical analysis, 2nd ed. Prentice-Hall, Englewood Cliffs, 718pp
Zieler H, Keister DB, Dvorak JA, Ribeiro JMC (2001) A snake venom phospholipase A2 blocks malaria parasite development in the mosquito midgut by inhibiting ookinete association with the midgut surface. J. Exp. Biol 204:4157–4167
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The authors thank Dr. Adalberto José Crocci for the statistical analysis. The experiments comply with current Brazilian laws.
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Shinohara, L., de Freitas, S.F., da Silva, R.J. et al. In vitro effects of Crotalus durissus terrificus and Bothrops jararaca venoms on Giardia duodenalis trophozoites. Parasitol Res 98, 339–344 (2006). https://doi.org/10.1007/s00436-005-0037-3
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DOI: https://doi.org/10.1007/s00436-005-0037-3