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Allozyme analysis of genetic variation and polymorphism in Eubothrium salvelini and E. crassum (Cestoda: Pseudophyllidea) from alpine lakes

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Abstract

The genetic structure of the most abundant species in the genus Eubothrium, E. crassum and E. salvelini, was studied by allozyme analysis in order to provide a consistent diagnosis for these morphologically similar species, as well as to assess their genetic relatedness. The proportion of fixed allelic differences (56.3%, 9 of 16 loci) between the species falls within the range accepted for valid species. A strict host specificity of E. crassum and E. salvelini for their typical host (lake trout, Arctic charr, respectively) was found in the French localities examined (Lake Annecy, Lake Bourget). For E. salvelini, polymorphism with the established allelic profile was found in the acid phosphatase (ACP) system. No significant deviation from the Hardy-Weinberg equilibrium expectations for ACP were found in either of the E. salvelini populations, which suggests predominantly cross-fertilization of these hermaphrodites. Analysis of the standardized variance in allelic frequencies demonstrated almost no among population differentiation (estimator θ of Fst=−0.03) between E. salvelini populations.

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Acknowledgements

Thanks are due to V. Hamelet (INRA, Thonon) for help in fish sampling and examination. We are also grateful to V. Dudiňák (Parasitological Institute SAS, Košice) for technical help in preparing the figures. The study was supported by the Slovak Grant Agency VEGA (no. 2/4177/04) and the Grant Agency of the Czech Republic (no. 524/01/1314). The experiments conducted comply with the current laws of both the Slovak Republic and France.

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Authors and Affiliations

  1. Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic

    Viliam Šnábel & Vladimíra Hanzelová

  2. Institute of Parasitology, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Tomáš Scholz

  3. Institute of Hydrobiology, INRA, BP 511, 74203, Thonon, France

    Daniel Gerdeaux

  4. Station de Pathologie Aviaire et de Parasitologie, INRA, 37380, Nouzilly, France

    Jacques Cabaret

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  1. Viliam Šnábel
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  2. Vladimíra Hanzelová
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  4. Daniel Gerdeaux
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Correspondence to Viliam Šnábel.

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Šnábel, V., Hanzelová, V., Scholz, T. et al. Allozyme analysis of genetic variation and polymorphism in Eubothrium salvelini and E. crassum (Cestoda: Pseudophyllidea) from alpine lakes. Parasitol Res 93, 290–295 (2004). https://doi.org/10.1007/s00436-004-1129-1

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