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Functional morphology of the raptorial forelegs in Mantispa styriaca (Insecta: Neuroptera)

Abstract

The insect leg is a multifunctional device, varying tremendously in form and function within Insecta: from a common walking leg, to burrowing, swimming or jumping devices, up to spinning apparatuses or tools for prey capturing. Raptorial forelegs, as predatory striking and grasping devices, represent a prominent example for convergent evolution within insects showing strong morphological and behavioural adaptations for a lifestyle as an ambush predator. However, apart from praying mantises (Mantodea)—the most prominent example of this lifestyle—the knowledge on morphology, anatomy, and the functionality of insect raptorial forelegs, in general, is scarce. Here, we show a detailed morphological description of raptorial forelegs of Mantispa styriaca (Neuroptera), including musculature and the material composition in their cuticle; further, we will discuss the mechanism of the predatory strike. We could confirm all 15 muscles previously described for mantis lacewings, regarding extrinsic and intrinsic musculature, expanding it for one important new muscle—M24c. Combining the information from all of our results, we were able to identify a possible catapult mechanism (latch-mediated spring actuation system) as a driving force of the predatory strike, never proposed for mantis lacewings before. Our results lead to a better understanding of the biomechanical aspects of the predatory strike in Mantispidae. This study further represents a starting point for a comprehensive biomechanical investigation of the convergently evolved raptorial forelegs in insects.

Introduction

The insect legs are locomotory appendages in the first place. However, they can vary greatly in functional abilities and morphological attributes: for example, bees store pollen in a specialized basket at their hind legs (Martins et al. 2014), webspinners spin their homes with a spinning apparatus located in their front feet (Büsse et al. 2015), and cicadas jump using their enlarged hind legs (Gorb 2004). Nevertheless, all these insects still utilize their legs for walking as well. Here, another example for this multifunctionality is presented, the raptorial forelegs. These prehensile legs are used for prey capturing and can most commonly be divided into two different types within insects: chelate and subchelate raptorial forelegs legs (compare Fig. 1 in Weirauch et al. 2011). The chelate type—best known as 'crab claws'—is in insects present in some Reduviidae (Hemiptera; Weirauch et al. 2011). The sub-chelate type raptorial forelegs are best known for Mantodea (see Roy 1999; Wieland 2013 or Brannoch et al. 2017). However, they can be found in other insect groups, such as Hemiptera, Diptera and Neuroptera as well, representing a prominent example of convergent evolution (Brémond 1974; Simpson 1975; Poivre 1976; Castro-Huertas et al. 2019). All these insects are ambush predators, showing strong morphological adaptations regarding the use of their forelegs as predatory striking and grasping devices (Ferris 1940; Ulrich 1965; Matsuda 1970; Loxton and Nicholls 1979; Frantsevich 1998). At first glance, praying mantises and mantis lacewings (Neuroptera: Mantispidae) share numerous morphological similarities (Ferris 1940; Ulrich 1965; Frantsevich 1998): a very movable, and often elongated, prothorax to strike for large distances (Frantsevich 1998), strong leg muscles to perform a fast predatory strike (Ulrich 1965; Gray and Mill 1983, 1985), as well as elongated and spined leg articles to hold the prey (Loxton and Nicholls 1979; Roy 1999; Wieland 2013). However, Mantispidae in general, show some very unique behavioural and morphological adaptations (Aspöck et al. 1980; Aspöck and Aspöck 1994, 2007; Redborg 1998). Representatives of the sub-group Mantispinae, for example, are spider associated parasites (Kaston 1938; Redborg and MacLeod 1983; Rice 1986; Redborg 1998; Aspöck and Aspöck 2007; Haug et al. 2018); within this context one of the few detailed studies on functional leg morphology in neuropterans was conducted, describing the highly specialised leg of the first larval instar of a representative (Jandausch et al. 2018).

Fig. 1
figure 1

Lateral view of Mantispa styriaca

The adult mantis lacewing is a classic sit-and-wait ambush predator (Boyden 1983), highly specialised to a predatory lifestyle (Fig. 1), as described above. The prothorax is elongated and flexibly connected to the following mesothorax (Ferris 1940; Ulrich 1965). The foreleg suspension is adapted for reliable aiming and controlling of fast and powerful strikes. Furthermore, it must provide enough versatility to hold and handle the prey (Frantsevich 1998). The leg articles—especially femur, tibia, and tarsus—are strongly modified: the femur is thickened and thorned, the tibia is blade-like and the tarsus forms a rather immobile elongation of the tibia (Ferris 1940; Ulrich 1965). Morphological differences, especially in the occurrence of femoral and tibial spines, in fossil and extant representatives of the sub-groups of Mantispidae are multifarious and allow for evolutionary and/or phylogenetic implications (Shi et al. 2015; Pérez-de la Fuente and Peñalver 2019). But details on the anatomy of the raptorial foreleg’s (Frantsevich 1998) as well as functional implications are rare.

The material composition of the raptorial foreleg’s cuticle of any insect, was, to our knowledge, never investigated. Yet, Loxton and Nicholls (1979) indicated differences within the cuticle in Mantodea, due to either heavily sclerotized or thin and flexible cuticle at the base of femoral spines, that needs further investigation. However, the relevance of the material composition in a functional and/or biomechanical context, was shown in a number of studies for a variety of character systems in insects like: mouthparts (Büsse and Gorb 2018), thorax (Bäumler and Büsse 2019) or abdomen (Willkommen et al. 2015), but most often for wings (Gorb 1999; Haas et al., 2000; Appel and Gorb 2011; Rajabi et al. 2016) and legs (Peisker et al. 2013; Jandausch et al. 2018; Petersen et al. 2018; Büsse et al. 2019a, b). The main purpose of the study is therefore, to present a detailed description of the raptorial forelegs of Mantispa styriaca (Poda, 1761), including extrinsic and intrinsic musculature as well as the material composition of their cuticle. Based on these data, we propose a spring-actuated mechanism (catapult mechanism) as driving force for the predatory strike, never suggested for mantis lacewings before. This results in a better understanding of biomechanical aspects of the predatory strike in Mantispidae, additionally allowing for a comparison to that of the Mantodea. This study represents a starting point to investigate the convergent evolution of raptorial forelegs in insects, giving new insights from a biomechanically very interesting, but often overlooked character system.

Materials and methods

Adult Mantispa styriaca (Fig. 1), from the collection of the Museum of Natural History Vienna (Austria), were used. The specimens were stored in 70–80% ethanol. Prior to scanning, both in micro-computed tomography (µCT) and scanning electron microscopy (SEM), the samples were dehydrated in an ascending ethanol series and dried at the critical point using a fully automatic Leica EM CPD300 (Leica, Wetzlar, Germany) CPD system.

High resolution X-ray tomography (μCT) was carried out using a SkyScan 1172 desktop µCT scanner (Bruker micro-CT, Kontich, Belgium) at 40 kV and 250 μA, with images taken over a full 360° rotation (step size: 0.25°). The segmentation of the data was done in Amira 6.2 (Thermo Fisher Scientific, Waltham, USA) and for visualization, the open source 3D creation suite Blender 2.82a (Blender Foundation, Amsterdam, Netherlands) was used.

For scanning electron microscopy (SEM), the dried samples were sputter-coated with gold–palladium (10 nm thickness; Leica Bal-TEC SCD500, Leica Camera AG, Wetzlar, Germany). Afterwards, the samples were mounted on a rotatable sample holder (Pohl 2010) and examined in a Hitachi TM3000 (Hitachi Ltd. Corporation, Tokyo, Japan) scanning electron microscope at an accelerating voltage of 15 kV.

For confocal laser scanning microscopy (CLSM), the legs were dissected and embedded in glycerine (99.9%) on a glass slide and covered with a high-precision cover slip prior to scanning. For CLSM visualisation, a Zeiss LSM 700 (Carl Zeiss AG, Jena, Germany) was used. Four laser lines (wavelengths of 405, 488, 555 and 639 nm) and four emission filters (BP420-480, LP490, LP560, LP640 nm) were used to visualize the autofluorescence of the cuticle (cf. Fig. 1 in Büsse and Gorb 2018). Maximum intensity projections were combined using ZEN2008 software (Carl Zeiss Microscopy AG, Jena, Germany). For more information on using CLSM to determine the material properties of the insect cuticle, we refer to Michels and Gorb (2012). The maximum intensity projections of the CLSM analysis, result in visualization of combined autofluorescence signals in every single pixel, providing information about the presence of various components within the cuticle with different material properties (Andersen 1979; Vincent 2002; Michels and Gorb 2012). It is therefore possible, to estimate the material composition of the analysed cuticle. The following colour code can be assigned: (1) red autofluorescence—stiff sclerotized cuticle; (2) blue, green and red combined (resulting in pink, brownish, yellow, and green autofluorescence within the overlay)—stiff but more flexible cuticle, if compared with the previous one, often chitin dominated; (3) characteristic blue autofluorescence— rubber-like cuticle with likely high proportion of resilin. The results of the CLSM analyses described in the following section allow for a qualitative assessment only and does not represent a quantitative measurement.

For stacked photography, we used a custom-made 3D-printed illumination dome system (Bäumler et al. 2020) and an Olympus OMD 10mkII digital camera (Olympus K.K., Tokyo, Japan), equipped with a Leica 45 mm macro lens (Leica Camera AG, Wetzlar, Germany). All images were subsequently processed in Affinity Photo and Affinity Designer (Serif Ltd, Nottingham, United Kingdom).

Extrinsic leg musculature terminology follows the nomenclature introduced by Friedrich and Beutel (2008), intrinsic leg musculature terminology follows the nomenclature used in Jandausch et al. (2018). Additionally, all terms regarding the outer morphology of the raptorial foreleg follow the nomenclature introduced by Wieland (2013) for Mantodea. The plane of orientation for the legs of M. styriaca are assigned, as if the forelegs were orientated as a cursorial insect leg, i.e. stretched aside (in congruence with Gray and Mill 1985; Wieland 2013). Newly introduced terms not found in the literature are defined at their first appearance in the text and put in quotation marks (‘…’).

Results

External leg morphology

In general, the sub-chelate type of raptorial forelegs is featured. The coxa of the raptorial foreleg is strongly elongated and the foreleg suspension (‘coxo-thoracic joint'—ctj) consists of large membranous areas (Fig. 2), reaching further posterior in ventral than in dorsal direction. Furthermore, the fronto-coxa shows one articulatory point with the pleural process, and the pleural condyle is connected via a ball and socket type joint with the coxa (Frantsevich 1998). The coxa is covered with a vestiture of small setae along the dorsal, and more strongly along the ventral area (Fig. 2). Here, a distinct posterodorsal ‘rabbet’, consisting of a dorsal and a posterior notch; forming a rabbet-like slot is present on the surface (Figs. 1, 2).

Fig. 2
figure 2

Scanning electron microscopy micrograph of Mantispa styriaca, ventrolateral view of head, prothorax and raptorial forelegs. Ctj coxo-thoracic joint

A small number of setae can be found on the trochanter. The trochanter is linked to the femur via a bowl-shaped dorsal oriented joint, becoming narrower towards the pointy distal end. The femur is almost triangular, with a wider and thicker area in the middle. It shows a 'posteroventral carina' (pvc), equipped with a median row of spines (referred to as 'anteroventral spines'—avs; Fig. 3), running from the widest point in the middle up to the proximal end. The most prominent character is a large, at the base bended, spine on the anteroventral side—the 'subbasal spine' (sbs; introduced from Willmann 1990 as major spine)—at the base of the carina, approximately in the middle of the femur (Fig. 3). A vestiture of small setae is almost completely covering the femur. This coverage is dense at the base of the femur, on the dorsal, and the anterior side (except on the mentioned carina; Fig. 3). On the anterior side and in a close radius around the proximal area of the carina, only a small quantity of setae is developed (Fig. 3). The tibial base is widened and reinforced, before bending further distal into a sickle-shape, with a distinct blade-like ‘cutting edge’ (ce) on the ventral side (Figs. 2, 3). A patch of setae can be found on the dorsal base, a few setae along the dorsal side and at the base of the cutting edge. Furthermore, a long triangular-shaped area, densely packed with small and thickened setae is located on the anterior side of the tibia.

Fig. 3
figure 3

Scanning electron microscopy micrographs of Mantispa styriaca, forelegs. a, c anterior and posterior view of closed raptorial foreleg; b, d anterior and posterior view of open raptorial foreleg; e ventral view of femur. As area of setae, Avs anteroventral spines, Btn basitarsal notch, Ce cutting edge, Cx coxa, Datp distal anterotibial protrusion, Dptp distal posterotibial protrusion, Fe femur, Pvc posteroventral carina, Sbs subbasal spine, Ta tarsus, Ti tibia, Tr trochanter

Expanding and forming the tip of the sickle-shape, the first tarsal article is an elongation of the tibial sickle. Additionally, the distal tip of the tibia forms a smaller posterior and larger anterior thorn-like protrusion—'distal posterotibial protrusion' (dptp) and 'distal anterotibial protrusion' (datp). The latter (datp) placed into a 'basitarsal notch' (btn) on the anterior side (Fig. 3).

Material composition

The most prominent parts of the raptorial foreleg (femur, tibia, and tarsus), exhibit a mixture of very different material properties (Fig. 4). The anterior area of the femur is strongly sclerotized (Fig. 4a), whereas the posterior area is a patchwork of resilin-dominated and chitinous parts, showing a median area of resilin-dominated cuticle (‘femoral posterior pad’—fpp; Fig. 4e). At the base of the femur (anterior view; Fig. 4a), a v-shaped, strongly resilin-dominated area stretches dorsally (‘femoral dorsobasal pad’—fdbp; approx. 1/3 the length of the femur) and ventrally (‘femoral ventrobasal pad’—fvbp) up to the subbasal spine (approx. 1/2 the length of the femur) (Fig. 4a) —stretching onto the subbasal spine (‘subbasal spine pad’—sbsp) facing proximal (Fig. 4b). In contrast, the distal face of the subbasal spine is strongly sclerotised (especially at the base), so is the tip. The anteroventral carina with its spines, is embedded in sclerotised cuticle anteriorly and chitinous cuticle posteriorly; the carina itself is also strongly sclerotised (Fig. 4a, e). The sickle-shaped tibia is more sclerotised on its anterior face and shows a line of resilin-dominated cuticle running from proximal to distal on the posterior face ('tibial posterior pad'—tpp; Fig. 4e). Additionally, the base of the tibia is slightly thickened and chitinous, with one anteriorly located resilin-dominated pad ('tibial anterobasal pad'—tabp; Fig. 4d) and one posteriorly oriented at the distal end (Fig. 4c). The mentioned cutting edge of the tibia is strongly sclerotised. At the distal tip of the tibia, a sclerotised protrusion—'distal anterior protrusion' (dap)—on the anterior side is developed.

Fig. 4
figure 4

Maximum intensity projection (CLSM) of the raptorial foreleg of Mantispa styriaca. a anterior view; b enlarged view of subbasal spine, C enlarged view of the distal tibial protrusion, d enlarged view of the widened tibial base, e posterior view. Fdbp femoral dorsobasal pad, Fvdp femoral ventrobasal pad, Fpp femoral posterior pad, Sbsp subbasal spine pad, Tadp tibial anterobasal pad, Tpp tibial posterior pad

The entire setae on the femur and tibia show no auto-fluorescence, appearing black in the CLSM maximum intensity projection (Fig. 4); which is presumably due to the presence of melanin in the cuticle.

Musculature

In all following descriptions, the non-moving end of a muscle will be referred to as a point of origin (O) and its moving end will be referred to as a point of insertion (I). All names for muscles follow the nomenclature introduced by Beutel et al. (2014), supplemented with information from Jandausch et al. (2018). Muscles marked with an asterisk are newly described.

Extrinsic musculature (Fig. 5)

Fig. 5
figure 5

Musculature of Mantispa styriaca’s raptorial foreleg. Three-dimensional visualisation from µCT data, lateral view. At certain areas, the cuticle is displayed transparent, to get a better view of the musculature. a, e, d intrinsic musculature; b, c extrinsic musculature; a, b inner layer; c, e outer layer. Dtp dorsal tibial protrusion, M muscle, Pcm pleurocoxal musculature, Scm sternocoxal musculature, Vtc ventral tibial complex

Dorso-ventral musculature

Idvm15—M. pronoto-trochantinocoxalis

O: Anterior part of pronotum.

I: At anterior procoxal rim next to Ipcm5.

Pleuro-coxal musculature

Ipcm4—M. propleuro-coxalis superior

O: Anterodorsal area of propleura.

I: Lateral at procoxal articulation.

Ipcm5—M. propleuro-coxalis inferior

O: Posteroventral face of propleural apodeme.

I: At anterior procoxal rim next to Idvm15.

Ipcm6—propleuro-coxalis posterior

O: Anteroventral face of propleural apodeme.

I: Posterolateral procoxal rim.

Sterno-coxal musculature

Iscm1—M. profurca-coxalis anterior

O: Lateral face of profurca stem.

I: Median, at proximal procoxal wall.

Iscm3—M. profurca-coxalis medialis

O: Lateral face of profurca.

I: Anteromesal procoxal rim.

Intrinsic musculature (Fig. 5)

Coxa:

ctm—Coxal-trochanteral muscle

O: Posterolateral procoxal rim.

I: Ventral trochanteral protrusion, dorsal of the insertion of M20 and M21.

M20—M. pleura-trochanteralis

O: Anteroventral wall of distal coxal half.

I: Ventral trochanteral protrusion, anteroventral of ctm.

M21—M. coxa-trochanteralis medialis

O: Posteroventral wall of distal coxal half.

I: Ventral trochanteral protrusion, posteroventral of ctm.

M22—M. coxa-trochanteralis lateralis

O: Three bundles of muscles, at anterior and posterior wall of distal half of coxa and at dorsal area of proximal coxal rim.

I: Dorsal, at protrusion at trochanteral rim.

Trochanter:

M23—M. reductor femoris

O: Ventral area of proximal end of trochanter.

I: Ventral, at most proximal end of femur.

Femur:

M24a—dorsal median und ventral

M. depressor tibiae a.

O: Anterodorsal to ventral area of proximal 2/3 of femur.

I: Together with M24b with a tendon at the enlarged tibial basis.

M24b—dorsal lateral und ventral

M. depressor tibiae b.

O: Posterio-dorsal to anteroventral area of proximal 2/3 of femur.

I: Together with M24a with a tendon at the enlarged tibial basis.

M25—M. levator tibiae

O: On dorsal area at most proximal region of femur.

I: Dorsal, on proximal rim of tibia.

Tibia:

M26—M. depressor pretarsi

O: Dorsal area of distal tibia.

I: Probably on tendon to claw.

M24c*—M. depressor tibiae O: With two strains on the ventrolateral and ventromedian base of the tibia. I: At the tendon, originating from muscle M24a & M24b.

Discussion

As previously mentioned, mantis lacewings share many morphological similarities with praying mantises, while also having particular separating morphological features. Interestingly, the raptorial forelegs of mantis lacewings are highly flexible in the coxo-thoracic, but also the coxotrochanteral joint. As already described by Frantsevich (1998), this is supported by the large membranous area of the coxothoracic joint (Figs. 1, 2)—resembling a multiaxial ball-and-socket-like joint. This flexibility allows for various movements like grooming (Lequet 2018: 0:50–0:58), handling prey (Lequet 2018: 2:10–2:18; Granier 2019: 0:40–0:50), grasping or walking (Frantsevitch 1998). The trochanter appears to be almost completely fused with the femur (Fig. 2), restricting any movement between those two parts to a minimum and forming a trochanter-femur complex.

In contrast, the joint between coxa and trochanter allows for a high degree of freedom. While handling prey (Lequet 2018: 2:10–2:18; Granier 2019: 0:41–0:55), a rotation of the coxotrochanteral joint of about 35° can be observed. At the distal end of the coxa, the rabbet is formed, consisting of one dorsal and one posterior notch forming a slot (Fig. 2). It likely stabilizes the trochanter-coxa joint in resting position, by limiting the possible degrees of freedom to a minimum in the anterior and posterior direction (Lequet 2018: 0:00–0:10). Typically, mantis lacewings using their meso- and metathoracic legs for walking only, here stabilizing and keeping the forelegs in a safe position is of major importance (Lequet 2018). The mentioned high degree of flexibility is further supported, by the arrangement of extrinsic muscle bundles (Fig. 5), as previously discussed by Frantsevitch (1998). As described in Jandausch et al. (2018) and found in the present study as well, the muscle M22 consists of 3 muscle bundles—one originating anterior, one posterior and one in the center of the proximal coxa—all inserting at the same region of the trochanteral rim (Fig. 5). This separation in three bundles may lead to different hypothetical rotational movements of the trochanter-femur complex: (i) central bundle—general depression (ii) anterior and posterior bundles—adjustments of the respective spatial orientation. Here, handling of prey while feeding may be improved significantly. Muscles M20, M21 and CTM are functioning as antagonists to M22, elevating the trochanter-femur complex (Fig. 5).

In contrast to mantis lacewings, praying mantises show more than twice as many muscles that interact between thorax, coxa and trochanter (Gray and Mill 1985; Frantsevitch 1998). Although, the reasons are not clear yet, a few possibilities are already discussible. A higher number of different muscles could facilitate adjustments of the predatory strike to varying situations, to might improve hunting success. Furthermore, it is probably necessary to use the predatory forelegs for additional purposes, such as walking and increased load-carrying compared to M. styriaca.

Also functioning in a similar way to praying mantises, particular differences of the predatory strike and the raptorial forelegs are noticeable (Granier 2019: 1:28–1:35; Jo Alwood 2016: 1:20–1:30; Josephs 2017: 0:00–0:05). In contrast to praying mantises (Brannoch et al. 2017), M. styriaca has no distal tibial spur; as this seems also to be true for the fossil mantodean lineage (Dittmann et al. 2015; Hörnig et al. 2017). In modern praying mantises, this tibial spur is described to cooperate with the discoidal spine(s), to lock the prey item when the tibia closes on the femur (Fig. 2 in Loxton and Nicholls 1979). Furthermore, the discoidal spine is described as surrounded by an area of soft cuticle and therefore, movable in distal direction to the femur (Fig. 1 in Loxton and Nicholls 1979). When capturing prey, those two spines work as a lever and ratchet system, as described for praying mantises in Loxton (Fig. 5 in Loxton and Nicholls 1979). The same mechanism is conceivable, in M. styriaca’s raptorial forelegs. As the lock-like structure, formed by the distal tibial protrusions (dptp, datp in Fig. 3b, d) together with the basitarsal notch, is noticeably sclerotized (Fig. 4c, e), it expands and elongates the sickle-shape of the tibia. Likely it has a similar purpose as the tibial spur in praying mantises. Additionally, the material composition of M. styriaca’s subbasal spine supports this assumption, as it shows a resilin-dominated area basally (sbsp in Fig. 4a, b), comparable to the discoidal spine(s) described for praying mantises. The distal face of the subbasal spine, the ventral surface, as well as the lateral base appear to be highly sclerotized (Fig. 4b, e). The medial and posteromedial face, as well as the median base and the following ventral area of the femur in contrast, appear to be resilin-dominated (Fig. 4b, e). This indicates a similar mechanism—a lever and ratchet system—formed by the elongated sickle-shape of tibia and tarsus in M. styriaca, resembling what is described for praying mantises by Loxton and Nicholls (1979). The anteroventral carina with its spines seems to work as a unit, with different possible functions. On the one hand, the strongly sclerotized surface, in interaction with the sclerotized cutting edge of the ventral tibia (ce in Fig. 4d), might work in a similar way as the carnassial teeth in carnivorous Mammalia (Mellett 1981). This mechanism could be used to get a hold on the prey, but also maybe breaking its outer cuticle, similar to the mentioned carnassial's. On the other hand, it could fix the spatial orientation of the tibia, ensuring its grip on the prey. In this case, the subbasal spine, in combination with a notch located anteroventral at the distal end of the femur, prevents the tibia from moving anteriorly. Additionally, the posteroventral carina with the anteroventral spines prevent the tibia from moving posteriorly (Fig. 3a, b). The subbasal spine, described here in detail for M. styriaca (Figs. 3, 4a, b), is not present in all extant Mantispidae (see Plega signata (Hagen, 1877) in Ferris 1940), but well known from the fossil record of, for example, Depranicinae (Shi et al. 2015; Pérez-de la Fuente and Peñalver 2019). As elaborately described by Shi and colleagues (2020) this spine seems to be a prominent character of the group comprising Depranicinae, Calomantispinae and Mantispinare, a well-established sister group relationship within Mantispidae (Liu et al. 2014).

Different setups of spines on the sub-chelate type raptorial forelegs are also present in the hemipteran group Reduviidae (Weirauch et al. 2011; Zhang et al. 2016; Castro-Huertas et al. 2019), including enlarged spines that might resemble the here, and in mantises (Loxton and Nicholls 1979), described system.

Outside Insecta, enlarged spines to capture prey with sub-chelate type raptorial forelegs are well-known from mantis shrimps (Crustacea: Stomatopoda). These spines are most likely strongly sclerotised and immobile because they are used to spear prey (Caldwell and Dingle 1975; deVries et al. 2012).

The mentioned sickle-shaped tibia of M. styriaca seems to be present in Mantispidae in general (Ferris 1940; Shi et al. 2015, 2020; Pérez-de la Fuente and Peñalver 2019). In M. styriaca no tibial spines are present; in general, no to minute spines on the tibial surface are present in Mantispidae (Pérez-de la Fuente and Peñalver 2019). This tibial condition differs significantly from the one found in Reduviidae (Weirauch et al. 2011; Zhang et al. 2016; Castro-Huertas et al. 2019) and Mantodea (Roy 1999; Wieland 2013 or Brannoch et al. 2017), both groups showing a significant density of spines on both the femur and the tibia. In Belostomatidae and Nepidae (Insecta: Hemiptera), however, sub-chelate type raptorial forelegs show no spines at all, neither on the tibia nor the femur (Schuh and Slater 1995).

The muscle set-up and movement sequence of the femur and tibia are of particular interest. The thin and long muscle M25 is running dorsally from the most proximal region of the femur to the proximal tibial rim, enabling the opening of the tibia – abduction (Fig. 5e, 6). Its large antagonists M24a and M24b, are capable of a fast and powerful closing of the tibia—adduction (Figs. 5a, e, 6). Together, these latter two muscles cover approximately 2/3 of the inner surface of the femur (Fig. 5a, e), converging at the distal end of the femur into a shared tendon. This tendon separates in two independent tendons at its apical end, attaching to the anterodorsal and posterodorsal proximal inner surface of the widened tibial base. Furthermore, muscle M24c originates at the lateral and median side of the ventral inner surface of the widened tibial base (Figs. 5a, 6), attaching to the mentioned tendon.

Fig. 6
figure 6

Functional schematic of the tibial catapult mechanism. a opening of the tibia; b loading of the catapult mechanism; c releasing of the catapult mechanism; d, e 3D schematic of the hypothetical muscle tendon complex of the catapult mechanism. d catapult unloaded; e catapult loaded. Dtp dorsal tibial protrusion, M muscle, Vtc ventral tibial complex

In combination with two cuticular protrusions (dorsotibial protrusion—dtp and ventrotibial complex—vtc), these four muscles likely represent a latch-mediated spring-actuated mechanism (Longo et al. 2019). This catapult mechanism is proposed here for mantis lacewings for the first time. Catapult systems are well known to operate ultra-fast predatory strikes, for example in dragonfly larvae (Büsse et al. 2021), or mantis shrimps (Patek et al. 2004). Here, it is functioning comparable to the described catapult systems in cicadas (Gorb 2004) or trap-jaw ants (Gronenberg 1999). Figure 6 shows a functional schematic of the hypothetical action of all structures involved in the process. At first, muscle M25 (blue) contracts, applying force to the dorsoproximal tibial rim, opening the tibia (Fig. 6a). Simultaneously, the 'dorsotibial protrusion' (dtp in Fig. 6b) deflects the tendon (green) in ventral direction, while the 'ventrotibial complex' (vtc in Fig. 6b)—this triangular sclerite is shaped like the collar bone (clavicle) of birds—deflects the tendon in dorsal direction (Fig. 6b). The tendon is deformed—absorbing kinetic energy, by the contraction of muscles M24a and M24b (Fig. 6b, d, e). Simultaneously, the shape of the ventrotibial complex becomes important; this triangular sclerite shows an anterior and posterior protrusion with a median gap proximally (Fig. 6d, e). To allow for spring actuation, the trigger muscle M24c contracts, pulling the tendon in lateral direction (Fig. 6b, e). At this point, the system is fully loaded and ready to strike, with the trigger muscle M24c in contracted state (Fig. 6b, e). To launch the spring, the trigger muscle M24c relaxes, causing the tendon to slide in ventromedial direction (Fig. 6c, d). Thereby, the two parts of the tendon are pulled over the edge of the anterior and posterior protrusion into the median gap of the ventrotibial complex (Fig. 6c, d). By crossing the axis of rotation, the tendon now returns into its original shape, releasing the previously stored energy and causing the fast-closing movement of the tibia (Fig. 6c). The video footage supports our morphological findings. When catching prey, the tibia of M. styriaca is always fully opened, before movements of the remaining parts of the predatory foreleg takes place—representing the loading of the catapult mechanism (Granier 2019: 1:27).

Conclusion

Using µCT analysis, we could confirm all previously described muscles for mantis lacewings, including one important new muscle—M24c. Considering the intrinsic musculature, the muscle setup in adult M. styriaca is different to, for example, previous descriptions for praying mantises. Additionally, we revealed a key feature of the raptorial forelegs in M. styriaca, previously only known for praying mantises: the subbasal spine in collaboration with the elongated sickle shape, formed by the tibia together with the tarsus, working as a lever and ratchet system, which is supported by the material properties indicated by our CLSM analysis. Furthermore, we proposed a latch-mediated spring-actuated (catapult) mechanism for the closing process of the tibia on the femur, unknown for mantis lacewings before. Our investigation resulted in new insights into the morphology of the raptorial forelegs and the functional mechanism of the predatory strike in mantis lacewings. Nevertheless, our study is only a door opener, to prove the proposed catapult mechanism in M. styriaca experimentally.

Data availability

All data supporting our findings are presented in the paper and the supplementary material respectively. The raw data of the CT- and CLSM-analysis can be made available on reasonable request.

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Acknowledgements

We are grateful for the support by the members of the Functional Morphology and Biomechanics Group at Kiel University, especially to T. H. Büscher, L. M. Hindenberg and A. Koehnsen. Many thanks to S. Randolf, Natural History Museum Vienna, Austria for providing specimens.

Funding

Open Access funding enabled and organized by Projekt DEAL. SB is directly supported through the DFG grants BU3169/1-1 & 1-2.

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SB and SNG designed the project and developed the concept of the study. FB and SB did the µCT analysis and post-processing. SB carried out the CLSM analysis. All authors wrote the manuscript as well as read and approved the final version.

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Correspondence to Sebastian Büsse.

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Büsse, S., Bäumler, F. & Gorb, S.N. Functional morphology of the raptorial forelegs in Mantispa styriaca (Insecta: Neuroptera). Zoomorphology 140, 231–241 (2021). https://doi.org/10.1007/s00435-021-00524-6

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Keywords

  • Mantis fly
  • Lacewing
  • Striking legs
  • Predatory strike
  • Catapult mechanism
  • Spring actuation
  • Insect cuticle