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Kinetid structure in larval and adult stages of the demosponges Haliclona aquaeductus (Haplosclerida) and Halichondria panicea (Suberitida)

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Abstract

At larval and adult stage of life cycle, sponges (Porifera) have different flagellated cells that play different roles in their body. The larval epithelium cells serve as locomotion organs, while adult collar cells (choanocytes) have a feeding function. Here, for the first time, we describe detailed structure of the flagellar apparatus (kinetid) in larval cells and choanocytes of Haliclona aquaeductus and Halichondria panicea demosponges, and attach schemes of its organization, based on longitudinal and transversal ultrathin serial sections. The kinetid of larvae has proved to be more complicated than the choanocytes ones. It includes well-developed transverse and longitudinal cytoskeletons that strengthen the larval flagellum. The longitudinal skeleton in H. aquaeductus is represented by unusual structures, which were previously understudied: large tubular rootlets made of oblique-crossed fibers, which connect the kinetosome with mitochondria. We also found out that the kinetid composition varies between different cell types of a highly structured parenchymella of H. aquaeductus. In simply organized parenchymella of H. panicea, flagellated cells are identical and its kinetid includes a permanent additional centriole absent in choanocytes of adult sponges. We tried to evaluate the diversity of sponge larval kinetid organization based on literature data and found it variable within Haplosclerida and quite conservative within other sponge lineages.

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Acknowledgements

The research was supported by the Russian Foundation for Basic Research (projects nos. 17-304-50015 and 18-04-01314). We thank Research Resource Center for Microscopy at Zoological Institute of Russian Academy of Sciences and Research Resource Center for Molecular and Cell Technologies (RRC MCT) at St. Petersburg State University for access to the EM facilities. We are also grateful to A. B. Tsetlin and personnel of White Sea Biological station (WSBS MSU) for assistance.

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Authors and Affiliations

  1. A. N. Severtzov Institute of Ecology and Evolution, Leninskij prosp. 33, Moscow, 119071, Russia

    Agniya M. Sokolova

  2. N. K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova street 26, Moscow, 119334, Russia

    Agniya M. Sokolova

  3. Biological Faculty, St. Petersburg State University, Universitetskaya emb. 7/9, St. Petersburg, 199034, Russia

    Igor R. Pozdnyakov, Alexander V. Ereskovsky & Sergey A. Karpov

  4. Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale (IMBE), Avignon University, Station Marine d’Endoume, Aix Marseille University, CNRS, IRD, rue de la Batterie des Lions, 13007, Marseille, France

    Alexander V. Ereskovsky

  5. Zoological Institute of Russian Academy of Science, Universitetskaya emb. 1, St. Petersburg, 199034, Russia

    Sergey A. Karpov

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  1. Agniya M. Sokolova
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  2. Igor R. Pozdnyakov
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Correspondence to Agniya M. Sokolova.

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Sokolova, A.M., Pozdnyakov, I.R., Ereskovsky, A.V. et al. Kinetid structure in larval and adult stages of the demosponges Haliclona aquaeductus (Haplosclerida) and Halichondria panicea (Suberitida). Zoomorphology 138, 171–184 (2019). https://doi.org/10.1007/s00435-019-00437-5

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