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Effects of galanin(1–16) on pancreatic secretion in anesthetized and conscious rats

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Research in Experimental Medicine

Abstract

Galanin, a 29-amino acid peptide, has been demonstrated in pancreatic nerve endings and found to inhibit insulin release in the rat. However, the data available concerning its effects on exocrine pancreatic secretion are contradictory. The aim of the present study was to evaluate the effects of a synthetic porcine galanin sequence, Gal(1–16), on stimulated pancreatic secretion in hyperglycemic anesthetized and conscious rats. Male Wistar rats were anesthetized and surgically prepared with pancreatic and femoral vein catheters. In anesthetized animals, the pancreatic secretion was continuously stimulated with 150 ng cholecystokinin octapeptide (CCK-8)/kg body weight per 30 min, dissolved in saline or 10% glucose. Synthetic Gal(1–16) (0.3 or 1 nmol/kg per h) was infused over a 60-min period. In conscious rats, 1, 3, or 10 nmol Gal(1–16)/kg per h was administered in a continuous saline or 10% glucose infusion over a 30-min period. The pancreatic secretory volume and protein output were determined in 30-min samples in both models. In anesthetized rats, 0.3 nmol Gal(1–16)/kg per h did not modify pancreatic secretion during CCK-8 stimulation. However, both the pancreatic secretory volume and the protein output were significantly inhibited compared with the basal levels by 1 nmol Gal(1–16)/kg per h. The inhibitory effect of Gal(1–16) on pancreatic secretion was more marked with CCK-8/glucose (53.9%) than with CCK-8/saline stimulation (20.1%). In conscious rats, significant inhibitory effects of 1 nmol Gal(1–16)/kg per h in saline were observed (18%). During glucose infusion, a dose-dependent inhibition of 1, 3, and 10 nmol Gal(1–16)/kg per h on pancreatic secretory volume and protein output (35% inhibition at 1 nmol/kg per h) was observed. In conclusion, the inhibitory effect of Gal(1–16) on exogenous and endogenous CCK-stimulated pancreatic secretion was found to be more potent in the presence of glucose both in anesthetized and in conscious rats. These results may suggest an indirect (insulin-mediated) inhibitory effect of porcine Gal(1–16) on pancreatic secretion in the rat.

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References

  1. Ahren B, Andren-Sandberg A, Nilsson A (1988) Galanin inhibits amylase secretion from isolated rat pancreatic acini. Pancreas 3:559–562

    Article  CAS  PubMed  Google Scholar 

  2. Bauer FE, Adrian TE, Christofides ND (1986) Distribution and molecular heterogeneity of galanin in human, guinea-pig and rat gastrointestinal tracts. Gastroenterology 91:877–883

    CAS  PubMed  Google Scholar 

  3. Bauer FE, Zintel A, Kenny MJ, Calder D, Ghatei MA, Bloom SR (1989) Inhibitory effect of galanin on postprandial gastrointestinal motility and gut hormone release in humans. Gastroenterology 97:260–264

    CAS  PubMed  Google Scholar 

  4. Brodish RJ, Kuvshinoff BW, Fink AS, McFadden DW (1994) Inhibition of pancreatic exocrine secretion by galanin. Pancreas 9:297–303

    Article  CAS  PubMed  Google Scholar 

  5. Bruzzone R, Trimble ER, Gjinovci A, Renold AE (1984) Glucose-insulin interactions on exocrine secretion from the perfused rat pancreas. Gastroenterology 87:1305–1312

    CAS  PubMed  Google Scholar 

  6. Fölsch UR, Cantor P, Wilms HM, Schafmayer A, Becker HD, Creutzfeldt W (1987) Role of cholecystokinin in the negative feedback control of pancreatic secretion in conscious rats. Gastroenterology 92:449–458

    PubMed  Google Scholar 

  7. Fölsch UR, Winckler K, Wormsley KG (1978) Influence of repeated administration of cholecystokinin and secretin on the pancreas of the rat. Scand J Gastroenterol 13:663–671

    Article  PubMed  Google Scholar 

  8. Green MG, Lyman RL (1972) Feedback regulation of pancreatic enzyme secretion as a mechanism for trypsin inhibitor induced hypersecretion in rats. Proc Soc Exp Biol Med 140:6–12

    Article  CAS  PubMed  Google Scholar 

  9. Gregersen S, Hermansen K, Yanaihara N, Ahrén B (1991) Galanin fragments and analogues: effects on glucose-stimulated insulin secretion from isolated rat islets. Pancreas 6:216–220

    Article  CAS  PubMed  Google Scholar 

  10. Herzig KH, Brunke G, Schön I, Schaffer M, Fölsch UR (1993) Mechanism of galanin’s inhibitory action on pancreatic enzyme secretion: modulation of cholinergic transmission — studies in vivo and in vitro. Gut 34:1616–1621

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Kanno T, Ueda N, Saito A (1976) Insuloacinar axis: a possible role of insulin potentiating the effects of pancreozymin in the pancreatic acinar cell. In: Fujita T (ed) Endocrine gut and pancreas. Elsevier, Amsterdam, pp 335–345

    Google Scholar 

  12. Kaplan LM, Spindler ER, Isselbacher JK, Chin WW (1988) Tissue-specific expression of the rat galanin gene. Proc Natl Acad Sci USA 85:1065–1069

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Kashimura J, Shimosegawa T, Kikuchi Y, Yoshida K, Koizumi M, Mochizuki T, Yanaihara N, Toyota T (1994) Effects of galanin on amylase secretion from dispersed rat pancreatic acini. Pancreas 9:258–262

    Article  CAS  PubMed  Google Scholar 

  14. Lee KY, Zhou L, Ren XS, Chang TM, Chey WY (1990) An important role of endogenous insulin on exocrine insulin on exocrine pancreatic secretion in rats. Am J Physiol 258: G268–274

    CAS  PubMed  Google Scholar 

  15. Lindskog S, Ahren B (1987) Galanin effect on basal and stimulated insulin and glucagon secretion in the mouse. Acta Physiol Scand 129:305–309

    Article  CAS  PubMed  Google Scholar 

  16. Lindskog S, Ahren B, Dunning BE, Sundler F (1991) Galanin-immunoreactive nerves in the mouse and rat pancreas. Cell Tissue Res 264:363–368

    Article  CAS  PubMed  Google Scholar 

  17. Lowry OH, Rosenburgh NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  18. Manabe T, Yoshimura T, Kii RC (1986) Galanin-induced hyperglycaemia: effect on insulin and glucagon. Endocrinol Res 12:93–98

    Article  CAS  Google Scholar 

  19. McDonald TJ, Dupré J, Tatemoto K, Greenberg GR, Radziuk J, Mutt V (1985) Galanin inhibits insulin secretion and induces hyperglycaemia in dogs. Diabetes 34:192–196

    Article  CAS  PubMed  Google Scholar 

  20. Otsuki M, Williams JA (1982) Effect of diabetes mellitus on the regulation of enzyme secretion by isolated rat pancreatic acini. J Clin Invest 70:148–156

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Rossowski WJ, Zacharia S, Jiang NY, Mungan Z, Mills M, Ertan A, Coy DH (1993) Galanin: structure-dependent effect on pancreatic amylase secretion and jejunal strip contraction. Eur J Pharmacol 240:259–267

    Article  CAS  PubMed  Google Scholar 

  22. Rünzi M, Müller MK, Schmid P, von Schonfeld J, Goebell H (1992) Stimulatory and inhibitory effects of galanin on exocrine and endocrine rat pancreas. Pancreas 7:619–623

    Article  PubMed  Google Scholar 

  23. Saito A, Williams JA, Kanno T (1980) Potentiation of cholecystokinin-induced exocrine secretion by both exogenous and endogenous insulin in isolated and perfused rat pancreata. J Clin Invest 65:777–782

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  24. Sofrankova A, Dockray GJ (1983) Cholecystokinin- and secretin-induced pancreatic secretion in normal and diabetic rats. Am J Physiol 244: G370–G374

    CAS  PubMed  Google Scholar 

  25. Takács T, Pap A, Varró V (1986) Effects of somatostatin on basal and stimulated pancreatic secretion in conscious rat. Acta Med Hung 43:45–53

    PubMed  Google Scholar 

  26. Takács T, Nagy I, Pap A, Varró V (1988) The anti-CCK effect of glutaramic acid derivatives in anesthetized and conscious rats. Pancreas 3:465–470

    Article  PubMed  Google Scholar 

  27. Tatemoto K, Rökaeus A, Jörnvall H, McDonald TJ, Mutt V (1983) Galanin — a novel biologically active peptide from porcine intestine. FEBS Lett 164:124–128

    Article  CAS  PubMed  Google Scholar 

  28. Verchere CB, Kwok YN, Brown JC (1992) Stimulus-specific inhibition of insulin release from rat pancreas by both rat and porcine galanin. Life Sci 51:1945–1951

    Article  CAS  PubMed  Google Scholar 

  29. Yagci RV, Altekin N, Zacharia S, Coy DH, Ertan A, Rossovsky WJ (1991) Galanin inhibits pancreatic amylase secretion in pentobarbital-anaesthetized rats. Regul Pept 34:275–282

    Article  CAS  PubMed  Google Scholar 

  30. Zeng-fa Gu, Rossowski WJ, Coy DH, Pradhan TK, Jensen RT (1993) Chimeric galanin analogs that function as antagonists in the CNS are full agonists in gastrointestinal smooth muscle. J Pharmacol Exp Ther 256:912–918

    Google Scholar 

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Authors and Affiliations

  1. First Department of Medicine, Albert Szent-Györgyi Medical University, 469, H-6701, Szeged, Hungary

    T. Takács, P. Hegyi, L. Czakó & J. Lonovics

  2. Central Research Institute of Chemistry of the Hungarian Academy of Sciences, Budapest, Hungary

    L. Baláspiri

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  1. T. Takács
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  2. P. Hegyi
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  3. L. Czakó
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  4. L. Baláspiri
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  5. J. Lonovics
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Takács, T., Hegyi, P., Czakó, L. et al. Effects of galanin(1–16) on pancreatic secretion in anesthetized and conscious rats. Res. Exp. Med. 199, 275–283 (1999). https://doi.org/10.1007/s004330050125

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  • DOI: https://doi.org/10.1007/s004330050125

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