Abstract
Objectives
Kidney renal clear cell carcinoma (KIRC) is one of the most common lethal cancers in the human urogenital system. As members of the Homeobox (HOX) family, Homeobox-A (HOXA) cluster genes have been reported to be involved in the development of many cancer types. However, the expression and clinical significance of HOXA genes in KIRC remain largely unknown.
Materials and methods
In this study, we comprehensively analyzed the mRNA expression and prognostic values of HOXA genes in KIRC using The Cancer Genome Atlas (TCGA) analysis databases online. Colony formation assay, flow cytometry and Western blot were used to detect cell proliferation, apoptosis, cell cycle, and protein level of the indicated gene.
Results
We found that the HOXA genes were differentially expressed in KIRC tissues when compared with normal tissues. The expression of HOXA4 and HOXA13 were significantly up-regulated, while HOXA7 and HOXA11 were down-regulated in KIRC. High mRNA levels of HOXA2, HOXA3 and HOXA13, and low level of HOXA7 predicted poor overall survival (OS) of KIRC patients. High mRNA level of HOXA13 further indicated a poor disease-free survival (DFS) of KIRC patients. Functionally, knockdown of HOXA13 significantly suppressed cell proliferation of KIRC in vitro, increased the protein level of p53 and decreased the protein level of cyclin D1 in KIRC cells. Over-expression of HOXA13 had the opposite effects on KIRC cells.
Conclusion
Collectively, our findings suggest that HOXA13 functions as a novel oncogene in KIRC and may be a potential biomarker for this malignancy.
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We thank all participants for this manuscript.
Funding
This work was supported by the Key Scientific Research Projects of Institutions of Higher Learning in Henan Province (20A310018), the Provincial Co-construction Project of Medical Science and Technology in Henan (SB201903032), the Key Scientific and Technological Project in Henan Province (182102310328).
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YBC conceived and designed this study and wrote the manuscript. MY, CYZ, JHX, QWZ and SSM participated in functional experiments and data analysis. WC and FXG assisted in the revision of the manuscript and sample collection. All authors read the manuscript and approved it for publication.
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Cui, Y., Yan, M., Zhang, C. et al. Comprehensive analysis of the HOXA gene family identifies HOXA13 as a novel oncogenic gene in kidney renal clear cell carcinoma. J Cancer Res Clin Oncol 146, 1993–2006 (2020). https://doi.org/10.1007/s00432-020-03259-x
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DOI: https://doi.org/10.1007/s00432-020-03259-x