Abstract
Purpose
To better understand the gene mutational status and heterogeneity between primary and metastatic CRC (mCRC) using a sensitive sequencing method.
Methods
The mutational status of EGFR, KRAS, NRAS, PIK3CA, ERBB2, BRAF, KIT, and PDGFRA was analyzed in 65 patients, with 147 samples of primary and paired live or lung metastatic CRC, using next-generation sequencing (NGS), quantitative RT-PCR (qPCR), and Sanger sequencing.
Results
Fifteen cases (15/22, 68.2%) of lung mCRC and thirteen cases (13/20, 65%) of liver mCRC harboured the same mutation profiles of KRAS, NRAS, or BRAF in the primary lesions. To all detected genes, 11 cases (11/22, 50%) of lung mCRC and 11 cases (11/20, 55%) of liver mCRC showed different mutational genes in the primary tumours. KRAS and BRAF mutations were more frequent in lung metastatic lesions (p = 0.004 and 0.003, respectively). The gene mutations in KRAS, NRAS, BRAF, and PIK3CA in the lung metastatic sites were more frequent than those in the liver metastatic sites (86.7 vs. 44%, respectively, p = 0.000). Some new mutations were not covered in the qPCR ranges but were detected by NGS.
Conclusion
The study demonstrated that the discordance of gene mutational status between paired primary and metastatic tumours is rather high when detected by NGS. Evaluating the mutational status of both the primary and metastatic tumours should be considered in clinical mutation testing.
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Introduction
Colorectal cancer (CRC) is one of the most common malignancies, and it is the fifth leading cause of cancer-related deaths in China (Chen et al. 2016). Invasion and metastasis account for the high mortality rate of CRC. Targeted therapies have transformed the current strategies in tumour treatment. Patients with metastatic CRC (mCRC) have greatly benefited from antibody-mediated blockade of the epidermal growth factor receptor (EGFR), with antibodies such as cetuximab and panitumumab (Dietel et al. 2015).
The EGFR signalling pathway plays a pivotal role in the proliferation and survival of CRC (Efferth 2012). The targeted drug that blocks EGFR is a therapeutic option in the treatment of mCRC. However, mutations in the genes downstream of the EGFR signalling pathway result in a constitutive activation of the EGFR and thus might abolish the effects of EGFR antibodies (Sinicrope et al. 2016). KRAS and NRAS are some of these well-known genes. BRAF and PIK3CA mutations may also be negative predictive biomarkers for anti-EGFR therapies (Nakayama et al. 2017). Moreover, other cancer-related genes, such as KIT and PDGFRA, may also act as important predictive and prognostic markers in CRC (Li et al. 2016). Therefore, it is essential to accurately detect the mutational status of CRC. The selection of suitable tissue samples is critical for accurate mutation detection but is challenging for pathologists (Van Cutsem et al. 2016). Some studies reported that the concordance in KRAS mutational status between the primary and the metastatic tumours is high, and tissue from either the primary tumour or metastasis may be used for testing mutations according to the latest NCCN guidelines for the treatment of mCRC (Han et al. 2012). However, some studies find that spatial and temporal tumour heterogeneity, which exists between the primary and the metastatic tumours, is significant and thus accounts for targeted therapy failure (Goswami et al. 2015).
Next-generation sequencing has been widely used in mutation detection in recent years, as it provides high analytic sensitivity and a broad reportable range (Haley et al. 2015). NGS has become a robust tool for molecular diagnosis of CRC (Kothari et al. 2014; Malapelle et al. 2015). Moreover, NGS can provide a quantitative measurement of mutant allele frequencies and predict tumour heterogeneity. In this study, we compared the mutational status between primary CRC and paired liver or lung metastasis by NGS, qPCR, and Sanger sequencing assays. We hope that this study may help in better understanding the mutational status and heterogeneity in mCRC and selecting suitable samples and technology for the accurate mutation detection of CRC by pathologists.
Materials and methods
Patient selection
This study included patients from a consecutive pathology database with surgically resected liver or lung mCRC in the Department of Pathology, Cancer Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College from 2007 to 2015, whose primary CRC was also resected at this hospital. Both the primary and metastatic resected specimens could be obtained for testing. At the time of surgical resection of mCRC, the patients had only lung or liver metastasis, but other metastatic sites, such as peritoneal, brain, ovary, etc., were not excluded. Patients who underwent neoadjuvant therapy before resection of primary tumours were not chosen for the study. All patients were not treated with cetuximab or other targeted agents before or after surgical resections of the primary or metastatic CRC.
Specimen selection
For each patient, every lesion of both primary and metastasis CRC was taken out, and the H&E slides were evaluated by two pathologists (SMZ and JMY). One of the representative paraffin blocks from each lesion was chosen for testing. A tumour content of more than 50% was usable for DNA extraction.
Genomic DNA extraction and quantitation
The genomic DNA from no less than five consecutive, 5 µm-thick slides of FFPE samples was extracted using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) per the manufacturer’s instructions. The DNA concentration was determined by the genomic DNA quantitative detection kit (Beijing ACCB Biotech Ltd., Beijing, China) on a CFX96 RT-PCR instrument (Bio-Rad, California, USA), and it was normalized to approximately 20–50 ng/µl. The DNA samples were stored at − 20 °C before utilization.
Mutation analysis by next-generation sequencing (NGS)
The NextDaySeq colon panel on the Ion TorrentTM System (Manufactured by Beijing ACCB Biotech, Beijing, China) was used to construct libraries according to the manufacturer’s instructions. Briefly, genomic regions of EGFR exons 18, 19, 20, and 21; KRAS exons 2 and 3; NRAS exons 2 and 3; PIK3CA exons 9 and 20; ERBB2 exon 20; BRAF exons 11 and 15; KIT exons 9, 11, 13, 14, and 17; and PDGFRA exons 12, 14, and 18 were amplified with pooled primer pairs, followed by ligations of the adaptors and barcodes. The libraries were purified and quantified using a Qubit dsDNA HS Assay Kit on a Qubit 2.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA); they were then diluted to a concentration of 3 ng/ml and pooled. The library pool was sequenced using the Ion Torrent PGM system. Briefly, the library pool was clonally amplified in an emulsion PCR reaction using Ion Sphere Particles on the OneTouch 2 instrument (Thermo Fisher Scientific). Template-positive ion sphere particles were enriched on the Ion OneTouch ES (Thermo Fisher Scientific). After enrichment, the sequencing primers and polymerase were added from the Ion PGM™ Sequencing Supplies 200 v2 Kit (Thermo Fisher Scientific). The libraries were loaded to generate sequencing data on the Ion 318 chips (Thermo Fisher Scientific) and sequenced on an Ion Torrent PGM (Thermo Fisher Scientific). A proprietary bioinformatics pipeline, the DanPA bioinformatics pipeline, was employed for variant calling and annotation. The pipeline was specifically designed for the NextDaySeq panels. The cutoff of mutation frequency for mutation calling was 1%, and that of mutation reads was 5.
Mutation analysis by quantitative RT-PCR (qPCR)
Mutation profiles of EGFR, KRAS, NRAS, BRAF, and PIK3CA were detected using the corresponding Human Gene Mutations Detection Kit, Human KRAS Gene Mutations Detection Kit, Human NRAS Gene Mutations Detection Kit, Human BRAF Gene Mutations Detection Kit, and Human PIK3CA Gene Mutations Detection Kit (Real-time Fluorescent PCR, Beijing ACCB Biotech Ltd., Beijing, China), respectively. The tests cover 72 hotspot mutations including 45 in exons 18, 19, 20, and 21 of EGFR; 12 in exons 2 and 3 of KRAS; 9 in exons 2 and 3 of NRAS; 5 in exons 9 and 20 of PIK3CA; BRAF V600E. The quantitative PCR was performed on an Mx3000P PCR instrument (Agilent, CA, USA) with the following settings: 95 °C for 10 min; 40 cycles of 95 °C for 15 s and 60 °C for 1 min. The results were interpreted as per the manufacturer’s instructions.
Mutation analysis by Sanger sequencing
The same genomic regions of eight genes covered in the NextDaySeq colon panel were amplified and sequenced bi-directionally with the ABI Prism Big Dye Terminator v 3.1 Cycle Sequencing Kit on an ABI 3130XL Genetic Analyzer (Thermo Fisher Scientific) according to the manufacturer’s instructions.
Definition of the gene mutation type
The wild type was defined as having no gene mutations detected in the gene panel by any of the gene sequencing methods. The mutant type was defined as having any mutation detected in the gene panel by any of the gene sequencing methods. A single mutation was defined as having only one gene mutation in the gene panel detected by any of the gene sequencing methods. The combined mutation was defined as having more than one gene mutation in the gene panel detected by any of the gene sequencing methods.
Statistics
Statistical analyses were performed with SPSS 25.0 software (SPSS, Chicago, IL). To evaluate the statistical significance of the differences observed between the groups, a Chi-square test was applied. To be specific, the Pearson’s chi-square test, the Fisher’s exact test, and continuity correction were used in different situations. All P values represent two-sided statistical tests and only those with less than 0.05 were considered significant.
Results
Basic characteristics of patients and primary CRC
Seventy-five patients with the primary and paired metastatic CRCs were chosen for the study. In ten of the cases, either the primary or the metastatic specimen failed the gene mutation test because of poor DNA quality. Finally, a total of 65 CRC patients, including 29 patients with lung metastasis and 36 patients with liver metastasis, were included in this study. They were divided into two groups according to the different metastasis locations. There were 42 males and 23 females; the median age was 58 years (30–78 years). No statistical difference was found between the two groups based on sex and age (p = 0.891). Some of the patients had more than one primary and metastatic CRC lesion. As a whole, 147 samples of primary CRC lesions and the paired metastases samples were analyzed.
There were 13 cases of primary CRC tumours that were located in the right half colon (caecum to transverse colon), 15 were located in the left half colon (descended to rectosigmoid colon), and 37 were located in the rectum. Fifty-six cases were well differentiated (G1/G2) and nine cases were poorly differentiated (G3/G4) according to the 2010 WHO classification of gastrointestinal tumours. Pathologic TNM classification was done according to the AJCC 7th. T3 took the majority between the two groups (48/65, 74%), followed by T4a (9/65, 14%). More than two-thirds of patients suffered from lymph node metastasis. Three cases had additional peritoneal metastasis besides liver metastasis. There was no significant difference between the two groups on the primary tumour characteristics, including location, differentiation, and T and N classifications. Twenty-nine and thirty-six cases had synchronous or metachronous distant metastasis, respectively. Synchronous metastasis was more common in the liver than it was in the lung metastasis group (p = 0.000). Therefore, the median time from primary tumour resections to metastasis resections was much longer in the lung metastasis group (23, 1–61 months) than that in the liver metastasis group (0, 0–35 months). The patient and primary tumour characteristics are summarized in Table 1.
Gene mutational status detected by qPCR, NGS, and Sanger sequencing
All the 147 samples were sequenced by both NGS and qPCR. Ninety-five samples were mutant type. The KRAS, NRAS, BRAF, PIK3CA, EGFR, KIT, and PDGFRA mutation detected by either NGS or qPCR was 54 (36.73%), 11 (7.48%), 6 (4.08%), 18 (12.24%), 3 (2.04%), 2 (1.36%), and 1 (0.68%), respectively. NGS detected more mutations than qPCR did (83 vs 76, respectively), especially for PIK3CA. NGS found 18 PIK3CA mutations, while qPCR only detected nine mutations. A total of 14 mutations were detected only by NGS, which are 1 KRAS L52P, 3 NRAS A91V, 1 BRAF G469A, 2PIK3CA E545A, 2 PIK3CA Q546K, 1 EGFR G735C, 1 EGFR G796S, 1 KIT W557*, 1 KIT P573L, and 1 PDGFRA M648fs (the details are shown in Table 2).
Sanger sequencing was performed in the first 16 tested cases, with 32 samples. Only 20 samples, including 9 wild type and 11 mutant type, showed the same results with NGS or qPCR method. Twelve samples were not consistent with NGS or qPCR results, which were nine samples mutant type in NGS or qPCR, but were wild type in Sanger sequencing. The other three cases were no results by Sanger sequencing. In addition, no mutations that were found by Sanger sequencing were not detected by NGS or qPCR. The results showed that the low sensitivity of Sanger sequencing compared with the other two methods.
Gene mutations in the primary and the metastatic CRC
Twenty-nine cases, with fifty-nine paired primary and lung metastatic CRC tissues, were analyzed. One case (No. 20) had two lung metastatic loci. Both the primary and the paired lung metastatic CRC were wild type in 7 patients (7/29, 24.1%). Gene mutations were found in the left 22 cases, 18 (18/29, 62.1%) in the primary sites, and 20 (20/30, 66.7%) in the metastatic sites. A total of 46 gene mutations were found, 20 (20/46, 43.5%) in the primary sites, and 26 (26/46, 56.5%) in the metastatic sites. In the primary sites, the gene mutations included 14 in KRAS, 2 in NRAS, 1 in PIK3CA, 2 in BRAF-V600E, 1 in KIT W557*, 1 in PDGFRA M648fs. The gene mutations in the metastatic sites were 17 in KRAS, 1 in NRAS, 3 in PIK3CA, 4 in BRAF, and 1 in EGFR (the details are shown in Table 3). The KRAS mutations were most frequent both in the primary and the metastatic CRCs (13/20, 65% and 17/26, 65.4%, respectively). Some mutations were detected by NGS and not included in the qPCR genomic regions, which were KIT W557*, PDGFRA M648fs, KRAS L52P, and BRAF G469A.
Thirty-six cases, with eighty-eight paired primary and liver metastatic CRC, were analyzed. Two cases had two primary sites; and three, two, and one cases had two, three, and eight metastatic sites, respectively. Both the primary and paired liver metastatic CRC were wild type in 16 patients (16/36, 44.4%). Gene mutations were found in 20 cases, 18 (18/20, 90%) from the primary site, and 17 (17/20, 85%) from the metastatic sites. A total of 49 gene mutations were found: 27 (27/49, 55.1%) from the primary sites and 22 (22/49, 44.9%) from the metastatic sites. There were more gene mutations in the primary sites than those in the metastatic sites. In the primary sites, gene mutations included 13 in KRAS, 5 in NRAS, 8 in PIK3CA, and 1 in EGFR. The gene mutations in the metastatic sites were 11 in KRAS, 3 in NRAS, 6 in PIK3CA, 1 in KIT, and 1 in EGFR. No BRAF mutation was found in either the primary or the liver metastatic CRC (the details are shown in Table 3). Some mutations were detected by NGS and were not included in the qPCR genomic regions, which were KIT P573L, EGFR G735C, and EGFR G796S.
When comparing the mutational gene status between the primary site of CRC with lung and liver metastasis (the details are shown in Table 3), we found that there was no significant difference in KRAS, NRAS, BRAF, and PIK3CA. Regarding the metastatic site, gene mutations in the lung metastatic sites were significantly much more prevalent than those in the liver metastatic sites (p = 0.000), with a total 26 gene mutations (26/30, 86.7%) and 22 gene mutations (22/50, 44%), respectively. We also found 17 and 11 KRAS gene mutation in the lung metastasis and liver metastasis, respectively (56.7 vs. 22%, p = 0.002).
The mutational consistency between paired primary and lung metastatic CRC
Fifteen cases (15/22, 68.2%) harboured the same mutation profiles between the primary and the lung metastatic lesions in KRAS, NRAS, and BRAF, which were 4 KRAS G12D, 6 KRAS G13D, 1 KRAS G12R, 1 KRAS G12A, 1 NRAS G12D, and 2 BRAF V600E. All were CRCs with metachronous metastasis. Eleven cases (11/22, 50%) harboured the exactly the same mutation between the primary and the lung metastatic lesions. The other 11 cases (11/22, 50%) showed somewhat different mutational genes (the details are shown in Table 4). Five of these cases showed gene mutation discrepancies beside KRAS, NRAS, and BRAF, which were EGFR L858R, PIK3CA H1047R, PIK3CA E542K, PDGFRA M648fs, and KIT W557*. In the other six cases, the gene mutation profiles were different in KRAS, NRAS, or BRAF, and five of them were metachronous metastasis. Only five cases underwent adjuvant chemotherapy or radiochemotherapy after primary tumour was resected, the gene mutation inconsistent cases were not statistical different between adjuvant and non-adjuvant therapy groups.
The mutational consistency between paired primary and liver metastatic CRC
Thirteen cases (13/20, 65%) harboured the same mutation profiles between the primary and the liver metastatic lesions in KRAS, NRAS, and BRAF, which were KRAS G12D, 1 KRAS G13D, 1 KRAS G12V, 1 KRAS Q61H, 1 NRAS G12D, 1 NRAS A91V, 1 PIK3CA E542K, 1 KRAS G13D and PIK3CA Q546K, and 1 PIK3CA H1047R and NRAS G12D. Nine cases (9/20, 45%) harboured the exactly the same mutation between the primary and the liver metastatic lesions. The other 11 cases (11/20, 55%) showed somewhat different mutational genes (the details are shown in Table 5). Six of these cases showed gene mutation discrepancies beside KRAS, NRAS, and BRAF, which were PIK3CA E545K, PIK3CA H1047R, EGFR G796S, and KIT P573L. In the other five cases, the gene mutation profiles were different in KRAS, NRAS, or BRAF. Most of these mutation inconsistent cases were with synchronous metastasis (9/11, 81.8%), there were 17 cases underwent adjuvant therapy. The mutation inconsistent cases were more in non-adjuvant therapy group than in adjuvant therapy group (9/19 vs 2/17, P = 0.021).
Gene mutation discrepancies among the separate primary or metastatic site(s) in one case
For those cases with more than one primary or metastatic site, each site was sequenced separately. We did observed that gene mutational status discrepancies were exist in CRC with liver metastasis. The details were summarized in Table 6. Case Liver 04 was a rectal cancer patient who suffered multiple simultaneous liver metastases in the sixth and seventh hepatic segment. The patient had a KIT P573L mutation only in the seventh hepatic segment lesion, while both the primary and the sixth hepatic segment metastatic lesions were wild type. Case Liver 28 was a patient with two primary lesions in the rectum and one simultaneous liver metastatic lesion in the seventh hepatic segment. The NRAS A91V mutation could be detected in all three lesions in this case, while the NRAS Q61R mutation was only detected in one of the primary lesions.
Discussion
KRAS and NRAS mutations are critical negative predictors of anti-EGFR therapy. In addition to KRAS and NRAS mutations, BRAF and PIK3CA mutations may also be potential biomarkers of resistance to anti-EGFR targeted treatments (Hsu et al. 2016; Karthaus et al. 2016). Some studies find that high concordance can be observed between the primary and metastatic tumours, suggesting that the detection of one lesion is sufficient to predict the response to EGFR-targeted therapy (Artale et al. 2008; Knijn et al. 2011; Santini et al. 2008). Therefore, the latest NCCN guideline strongly recommends genotyping of tumour tissue (either primary tumour or metastasis) in all patients with metastatic colorectal cancer for RAS and BRAF. However, most of these reports perform the mutation test using Sanger sequencing or ARMS-PCR, which may be limited by low sensitivity or detection of common mutations (Artale et al. 2008; Knijn et al. 2011; Santini et al. 2008). Compared with these traditional technologies, NGS can provide higher sensitivity and border detection ranges. Therefore, using NGS may better uncover the genetic heterogeneity between primary and metastatic tumours.
In this study, we detected the mutation profile between paired primary and liver or lung metastatic tumours using multiple sequencing methods. In general, NGS detected more mutations than qPCR did. We found that the mutation profile discordant rate was high between primary and metastatic CRC, which may influence the treatment decision on anti-EGFR target therapy. There were 11 cases (11/22, 50%) which showed different mutational genes between the primary and the lung metastatic tumours. The gene mutation profiles were different in RAS or BRAF in six of these cases. In four cases, tumours were wild type in primary sites, but were RAS mutant type in lung metastatic sites; in another 1 case, gene mutation changed from KRAS G12D and PIK3CA E545A in primary site to wild type in lung metastatic site. There were also 11 cases (11/20, 55%) which showed different mutational genes between the paired primary and liver metastatic CRCs. The gene mutation profiles were different in RAS or BRAF in five of these cases. Three cases were RAS mutant type in primary sites, but were RAS wild type in metastatic sites; on the contrary, another 1 case was RAS wild type in primary site, but was RAS mutant type in metastatic site. We also found that mutation inconsistent cases were much more in CRC with synchronous liver metastasis. These data strongly support the notion that testing the mutational status of metastatic tumours is necessary rather than only testing the mutational status of primary tumours to further guide targeted therapy selection, both in synchronous and metachronous metastatic cases.
Neoadjuvant or adjuvant radiochemotherapy may have potential effect on gene mutation status. Some studies tried to determine if there are any changes in KRAS mutation status before and after preoperative chemoradiotherapy (CRT) in primary CRC. The results were controversial. In the study by Boissiere-Michot et al., KRAS mutation was identified in 9/31 (29%) surgical specimens which major tumour regression is achieved in locally advanced rectal adenocarcinomas after radiochemotherapy, less than in the paired pretreatment biopsies (12/31, 39%) (Boissiere-Michot et al. 2012). Jo et al. did not obtain evidence that CRT results in changes of the KRAS mutation pattern and no intratumoural heterogeneity in the KRAS mutational status could be proven before and after neoadjuvant CRT. The discrepancy observed in these studies may be caused by the amount of viable tumour cells after CRT and the sensitivity of the analytical method (Jo et al. 2016). The gene mutation discrepancy between primary and metastatic tumour with or without adjuvant therapy is unknown. In the present study, there were 17 cases which underwent adjuvant chemotherapy in liver metastatic CRC group. We observed that the mutation inconsistent cases were more in non-adjuvant therapy group than in adjuvant therapy group (9/19 vs 2/17, P = 0.021). This data may show potential therapy-related changes, but it needs to be proven by further larger and well-designed studies.
The presence of tumour heterogeneity has been found in many cancers and is a possible explanation for therapy resistance (Xie et al. 2014). Several studies have demonstrated the genetic, epigenetic, and transcriptomic heterogeneity in CRC (Jones et al. 2017; Punt et al. 2017). In our study, we tested the mutational status between 65 paired primary and metastatic tumours, including 36 paired primary-liver metastatic tumours and 29 paired primary-lung metastatic tumours. We found that the discordant rate of mutational status between the paired primary and the metastatic tumours was as high as 55%. In addition, more gene mutations were detected in the lung metastatic tumours than those in the primary tumours. The possible explanation is that most lung metastatic tumours are metachronous metastasis. Discordant mutations may be acquired in the lung metastases because of clonal evolution following chemotherapy compared with that in the primary tumours (Findlay et al. 2016).
In addition to predicting the response to EGFR-targeted therapy, the KRAS, NRAS, BRAF, and PIK3CA mutations may also act as significant prognostic factors in CRC (Foltran et al. 2015; Vogelaar et al. 2015). Here, we found that the frequency of KRAS and BRAF mutations was higher in the lung metastatic tumours than that in the liver metastatic tumours. These data indicate that determining the mutation profiling of CRC may have a potential implication for CRC development and progression.
Moreover, some rare gene mutations were observed in our studies, such as the KIT and EGFR mutations. These rare mutations may also act as a potential target for the treatment of CRC, and application of NGS can comprehensively determine the mutational status of CRC (Chen et al. 2015; Cho et al. 2014). However, more studies are necessary to further explore the relationship between these rare mutations and target therapies.
This study has some limitations. First, it is a retrospective study of a single centre. Second, some samples were excluded for poor DNA quality and the number of samples was relatively small. Third, only the resection samples were in the study, which may show case selection bias, because those were all resectable or potentially resectable mCRC, which were in a relatively early stage.
In conclusion, our study demonstrates that the discordance of the gene mutational status between paired primary and metastatic CRC is rather high when detected by NGS. Therefore, evaluating the mutational status of both the primary and the metastatic tumours should be considered in clinical mutation testing to better guide targeted therapies of mCRC.
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Funding
This study was funded by: 1. National Key R&D Program of China (Grant No: 2016YFC0905300). 2. CAMS Innovation Fund for Medical Sciences (CIFMS) (Grant no.2017-I2M-1-006 and 2016-I2M-1-001). 3. Beijing Hope Run Special Fund of Cancer Foundation of China (Grant no. LC2014A08 and LC2017A07).
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SMZ designed and conducted the study, and wrote and submit the manuscript. WHL wrote some of the original manuscript. WMW performed the statistical analyses and wrote some of the original manuscript. WBL analyzed and interpreted of the experimental data. SSS collected, interpreted, and analyzed the clinical data. JMY designed and managed the study, and evaluated the H&E slides. NL designed and managed the study, and had final responsibility for the decision to submit for publication.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Cancer Hospital, Chinese Academy of Medical Sciences, National GCP Center for Anticancer Drugs, and The Independent Ethics Committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Zou, SM., Li, WH., Wang, WM. et al. The gene mutational discrepancies between primary and paired metastatic colorectal carcinoma detected by next-generation sequencing. J Cancer Res Clin Oncol 144, 2149–2159 (2018). https://doi.org/10.1007/s00432-018-2742-1
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DOI: https://doi.org/10.1007/s00432-018-2742-1