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Hox B4 as potential marker of non-differentiated cells in human cervical cancer cells

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Abstract

Background

Cervical cancer (CC) is a common malignancy in women worldwide. Cervical tumorigenesis involves a multistep process in which accumulations of genetic alterations are present. Homeotic genes, such as HOX gene re-expression, have been reported in a wide variety of tumors.

Methods

In order to know the role of HOX B4 gene expression in CC, in the present study, two-dimensional polyacrylamide gel electrophoresis, matrix-assisted laser desorption/ionization, and time-of-flight mass spectrometry were used for differential screening of protein expression in CC. Immunohistochemical analysis was performed on the cervical tissue microarray (TMA) to detect the Hox B4 protein.

Results

Hox B4 peptide was detected among 15 increased spots differentially observed in CC. Using TMA, Hox B4 protein was also immunodetected in the nuclei of cervical epithelial tumor cells, while in normal cervical epithelium, it was absent. Interestingly, it was possible to detect the Hox B4 protein in the precursor lesions.

Conclusions

Hox B4 protein is present in the precursor lesions as CC cells, suggesting that Hox B4 could be a protein related to the neoplastic state (non-differentiated cells) of human cervical epithelium.

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Acknowledgments

This work was partially supported by the Fondos Mixtos Guanajuato and San Luis Potosí, Mexico, and Fondos Sectoriales 87244 and 69719 CONACYT, Mexico. We thank Dra. Sara Fonseca Castañol for her invaluable help on this work.

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Authors and Affiliations

  1. Division de Biologia Molecular, Instittuto Potosino de Investigacion, Ciencia y Tecnologia, Mexico, SLP, Mexico

    Ana P. Barba-de la Rosa, Erika Briones-Cerecero, Ofelia Lugo-Melchor, Antonio De León-Rodríguez & Leticia Santos

  2. Departamento de Ginecologia y Obstetricia, Hospital Central “Ignacio Morones Prieto”, San Luis Potosí, SLP, Mexico

    Julio Castelo-Ruelas

  3. Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN S-XXI, IMSS, Av. Cuauhtémoc 330, Col. Doctores, Mexico, D.F., 06720, Mexico

    Alejandra Valdivia, Patricia Piña, Daniel Hernandez-Cueto & Mauricio Salcedo

  4. Departamento de Proteomica, CINVESTAV-IPN, U. Irapuato, Gto, Mexico

    Alicia Chagolla-López

  5. Servicio de Patologia, Hospital de Oncologia, CMNSXXI-IMSS, Mexico, Mexico

    Alejandra Mantilla

  6. Departamento de Patologia, Hospital General de Mexico, Mexico, SS, Mexico

    Minerva Lazos-Ochoa

  7. Departamento de Biologia Molecular, Universidad de Guanajuato, Leon, Gto, Mexico

    Beatriz Gonzalez-Yebra

Authors
  1. Ana P. Barba-de la Rosa
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  2. Erika Briones-Cerecero
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  3. Ofelia Lugo-Melchor
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  4. Antonio De León-Rodríguez
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  5. Leticia Santos
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  8. Patricia Piña
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  9. Alicia Chagolla-López
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  14. Mauricio Salcedo
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Corresponding author

Correspondence to Mauricio Salcedo.

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Barba-de la Rosa, A.P., Briones-Cerecero, E., Lugo-Melchor, O. et al. Hox B4 as potential marker of non-differentiated cells in human cervical cancer cells. J Cancer Res Clin Oncol 138, 293–300 (2012). https://doi.org/10.1007/s00432-011-1081-2

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  • DOI: https://doi.org/10.1007/s00432-011-1081-2

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