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Cell death-inducing effect of novel palladium(II) and platinum(II) complexes on non-small cell lung cancer cells in vitro

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Abstract

Purpose

Treatment for lung cancer is still far from satisfying rates. Therefore, there is a need for novel anticancer agents. For this purpose, novel platinum and palladium complexes {[Pd(sac)(terpy)](sac)·4H2O (Complex 1), [Pt(sac)(terpy)](sac)·5H2O (Complex 2), [PdCl(terpy)](sac)·2H2O (Complex 3), [PtCl(terpy)](sac)·2H2O (Complex 4)} have been tested against three different non-small cell lung cancer cell lines (A549, H1299, PC-3).

Methods

Growth-inhibiting effects have been tested by the MTT and ATP viability assays. Apoptosis has been detected by the caspase-cleaved cytokeratin 18 (M30-antigen) assay. Necrosis has been detected by staining the cells with fluorescent dyes. Mitotic index has been calculated by counting the mitotic figures after staining with hematoxylin.

Results

The complex 3 exhibited significant anti-growth effects, and its anti-growth effect was more powerful than that of cisplatin that is a standard chemotherapeutic agent for this type of cancer. The complexes did not induce apoptosis, while necrosis clearly took place.

Conclusions

Novel Pd(II) complex ([PdCl(terpy)](sac)·2H2O) seems to represent a potentially active drug against non-small cell lung cancer cell lines, and further studies in vivo are warranted.

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Acknowledgments

We thank the Research Fund of Uludag University for providing us with the kits/chemicals.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Medical School, Uludag University, 16059, Bursa, Turkey

    Engin Ulukaya

  2. Department of Biology, Faculty of Arts and Sciences, Uludag University, Bursa, Turkey

    Ferda Ari & Mehmet Sarimahmut

  3. Department of Pharmacology, School of Medicine, University of Thessaly, Biopolis, Larisa, Greece

    Konstantinos Dimas & Nikos Sakellaridis

  4. Department of Chemistry, Faculty of Arts and Sciences, Uludag University, Bursa, Turkey

    Emel Guney & Veysel T. Yilmaz

Authors
  1. Engin Ulukaya
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  2. Ferda Ari
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  3. Konstantinos Dimas
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  4. Mehmet Sarimahmut
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  5. Emel Guney
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  6. Nikos Sakellaridis
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  7. Veysel T. Yilmaz
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Correspondence to Engin Ulukaya.

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Ulukaya, E., Ari, F., Dimas, K. et al. Cell death-inducing effect of novel palladium(II) and platinum(II) complexes on non-small cell lung cancer cells in vitro. J Cancer Res Clin Oncol 137, 1425–1434 (2011). https://doi.org/10.1007/s00432-011-1021-1

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  • DOI: https://doi.org/10.1007/s00432-011-1021-1

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