Abstract
Purpose
The aim of this study was to investigate the role of Li-cadherin in invasion and metastasis in LoVo cells.
Methods
We applied RNA interference mediated downregulation of Li-cadherin expression in LoVo cells. Li-cadherin expression in LoVo cells was examined by semiquantitative polymerase chain reaction, immunofluorescence, western blot, and immunoprecipitation, respectively. Effect of suppression of Li-cadherin expression on cell migration, invasion, and adhesion was detected by wound healing assay, migration assay, invasion assay, and adhesion assay. Expression and activity of MMP-2 and MMP-9 were analyzed by gelatin zymography.
Results
Cell migration, invasion, and adhesion were increased concomitantly with the reduction in Li-cadherin protein expression. Furthermore, downregulation of Li-cadherin expression induced secretion of proMMP-9, active MMP-9 and active MMP-2.
Conclusions
This study suggests that silencing Li-cadherin has positive actions in the processes of LoVo cells invasion and metastasis, and the interactions among MMP-2, MMP-9, and Li-cadherin participate in the multiple steps of invasion and metastasis in LoVo colorectal cancer cells.
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Acknowledgments
We thank Ph.D. Dian Gao (Medical College, Nanchang University) for excellent technical assistance.
Conflict of interest statement
Authors declare that the manuscript is submitted on behalf of all authors. None of the material in this manuscript has been published previously in any form and none of the material is currently under consideration for publication elsewhere other than noted in the covering letter to the editor.
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Yu, QF., Dong, WG. & Ren, JL. Knockdown of Li-cadherin increases metastatic behaviors of LoVo cells. J Cancer Res Clin Oncol 136, 1641–1649 (2010). https://doi.org/10.1007/s00432-010-0822-y
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DOI: https://doi.org/10.1007/s00432-010-0822-y