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Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1

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Abstract

Purpose

To confirm the presence of the susceptible gene for the thymoma development in the region that was assumed by the previous linkage study by Oyabu et al. (J Natl Cancer Inst 91:279–282, 1999), we tried to establish a congenic strain of rats.

Methods

Backcrossings between the BUF/Mna strain as a donor strain and the ACI/NMna strain as an inbred partner were repeated for 12 generations, examining whether rats had the thymoma development region, and then homozygous rats were yielded by mating among the heterozygotes. To detect the phenotypic expression, heterozygous ACI.BUF/Mna-Tsr1/+ (ACI-Tsr1/+) rats were generated by crossing female ACI.BUF/Mna-Tsr1/Tsr1 (ACI-Tsr1/Tsr1) rats with male ACI/NMna rats and were maintained for 24 months.

Results

These ACI-Tsr1/+ rats produced thymoma in 71%, showing a dominant trait. The thymomas were of the lymphocyte predominant type, as those developed in rats of the original BUF/Mna strain.

Conclusions

Thus, a new rat congenic strain, ACI-Tsr1/Tsr1, was established, revealing that thymoma develops in the dominant trait in ACI-Tsr1/+ rats.

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Abbreviations

Tsr1 :

Thymoma susceptible locus of rat-1

ACI-Tsr1/Tsr1 :

ACI.BUF/Mna-Tsr1/Tsr1

ACI-Tsr1/+:

ACI.BUF/Mna-Tsr1/+

Chr:

Chromosome

PCR:

Polymerase chain reaction

SSLP:

Single strand length polymorphism

Ten-1 :

Thymus enlargement locus-1

Ten-2 :

Thymus enlargement locus-2

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Acknowledgments

We thank Prof. T. Serikawa for his advice, and Miss K. Ishikawa and Miss J. Adachi for their technical assistance. This work was supported by the Imanaga Medical Foundation. We are also thankful to the National Bioresource-Rat for keeping rats of the congenic strain.

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Authors and Affiliations

  1. Department of Surgical Pathology, School of Medicine, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake-shi, Aichi-ken, 470-1192, Japan

    Mutsushi Matsuyama & Makoto Kuroda

  2. Department of Pathology, School of Health Sciences, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake-shi, Aichi-ken, 470-1192, Japan

    Kazuo Kato

  3. Department of Virology and Parasitology, School of Medicine, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake-shi, Aichi-ken, 470-1192, Japan

    Kyoko Higo-Moriguchi

  4. Central Laboratories, Seirei Hamamatsu Hospital, 2-12-12 Sumiyoshi, Naka-ku, Hamamatsu-shi, Shizuoka-ken, 430-8558, Japan

    Tetsuji Yamada

  5. Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606-8501, Japan

    Takashi Kuramoto

Authors
  1. Mutsushi Matsuyama
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  2. Kazuo Kato
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  3. Kyoko Higo-Moriguchi
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  4. Tetsuji Yamada
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  5. Takashi Kuramoto
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  6. Makoto Kuroda
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Corresponding author

Correspondence to Mutsushi Matsuyama.

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Matsuyama, M., Kato, K., Higo-Moriguchi, K. et al. Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1 . J Cancer Res Clin Oncol 134, 789–792 (2008). https://doi.org/10.1007/s00432-007-0346-2

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  • DOI: https://doi.org/10.1007/s00432-007-0346-2

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