Abstract
Hyponatremia is a common disorder in childhood. The indirect and the direct potentiometry are currently the most popular techniques employed for sodium assessment, although discrepancies between the two techniques may be > 10 mmol/L. It is known that < 20% of the recently published articles report information about the technique used for sodium analysis, but no data are available on pediatric studies. This study aimed at investigating the laboratory technique employed for sodium measurement in studies conducted in childhood. A systematic literature search in PubMed, Embase, and Web of Science was undertaken to identify articles containing the word “hyponatremia” in the title between 2013 and 2020. Papers with < 10 subjects were excluded. A total of 565 articles were included. Information on the laboratory technique used for sodium analysis was more commonly (p = 0.035) reported in pediatric (n = 15, 28%) than in non-pediatric (n = 81, 16%) reports. The frequency of reports with and without information on the technique for sodium assessment was not different with respect to the study characteristics, the quartile of the journal where the paper was published, the country income setting, and the inclusion of neonates among the 54 pediatric studies.
Conclusion: Most pediatric papers do not report any information on the technique used for sodium analysis. Although international authorities have recommended the implementation of direct potentiometry, a low awareness on this issue is still widespread in pediatric research.
What is Known: |
---|
• Direct potentiometry and indirect potentiometry are currently employed for sodium analysis in blood. |
• Direct potentiometry is more accurate. |
What is New: |
• Less than 30% of pediatric articles provide information on the technique employed for sodium analysis in blood. |
• Indirect potentiometry is more frequently employed than direct potentiometry in pediatric studies. |
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Introduction
Hyponatremia is a frequent laboratory finding in childhood and may by complicated by neurological damage [1, 2]. With the gradual abandonment of flame photometry, indirect and direct potentiometry are nowadays the most popular techniques for sodium analysis in clinical practice and research [3].
The indirect potentiometry is usually employed by laboratory analyzers whereas the direct technique by “point-of-care testing” tools such as blood gas analyzers. Although the agreement between the two methods is considered good, the results obtained by indirect potentiometry may be falsely increased or decreased by up to 10 mmol/L [4, 5].
A recent systematic review found that > 80% of the published articles do not report information about the technique used for sodium analysis [6]. However, no similar data are available on pediatric studies. The aim of this analysis of the literature is to investigate if data on the laboratory technique employed for sodium measurement are reported in studies on children, which technique is more frequently used, and if there are differences with studies conducted in adults.
Methods
Literature search and study selection
A systematic search of the literature in the National Library of Medicine database (PubMed), Excerpta Medica database (Embase), and Web of Science was undertaken to identify articles containing the word “hyponatremia” or “hyponatraemia” in the title according to the 2020 PRISMA guidelines. Only articles published in English from 2013 to 2020 as full text were retained. Articles including < 10 cases or not conducted in humans were excluded. Two authors independently screened the articles for eligibility.
Data extraction and study classification
From retained articles, the following information was extracted: (1) population type (pediatric vs non pediatric), (2) number of included subjects, (3) laboratory technique employed for sodium assessment (flame photometry, direct potentiometry, indirect potentiometry, not reported), (4) data collection (retrospective vs prospective), (5) study design (observational vs interventional), (6) quartile of the journal where the paper was published, (7) country income setting (high versus other). Pediatric studies were also subdivided in those with and without neonates.
The Journal Citation Reports (Clarivate Analytics’ Web of Science™) was used to assign the quartile ranking journal where the paper was published, in the year of publication. If the journal was listed in more than one subject area, the highest quartile was chosen.
Outcomes
For the present study, the primary outcome was the percentage of pediatric papers reporting information on the technique used for sodium assessment. The secondary outcomes were (1) the percentage of the non-pediatric papers reporting information on the technique used for sodium assessment and (2) the characteristics of the papers reporting the information on the technique used for sodium assessment.
Data management and statistical analysis
Corresponding authors of each article with no information about the technique employed for sodium measurement were contacted to gather the information. A reminder was sent to non-responders 1.5–2 months after the first email. To manage missing data, pairwise deletion was used. For data analysis, the two-tailed Fisher exact test was employed to compare categorical data, and statistical significance was set at p < 0.05. Prism 9 was used for the analysis.
Results
Search results
The literature search process is reported in Fig. 1 and the full list of included papers is given in the supplementary online material (pediatric studies in supplementary file 1 and non-pediatric studies in supplementary file 2). Five hundred and sixty-five articles were included. They were published from the following continents: 225 from Asia (Japan, n = 46; India, n = 35; Korea, n = 33; China, n = 29; Pakistan, n = 19; Turkey, n = 16; Taiwan, n = 15; Israel, n = 12; Iran, n = 6; Saudi Arabia, n = 4; Indonesia, n = 2; Singapore, n = 2; Bangladesh, n = 1; Nepal, n = 1; Philippines, n = 1; Russia, n = 1; Thailand, n = 1; Vietnam, n = 1), 162 from Europe (Italy, n = 30; Spain, n = 19; Germany, n = 17; Switzerland, n = 14; Denmark, n = 13; Sweden, n = 11; France, n = 9; Belgium, n = 8; Netherlands, n = 8; Poland, n = 8; Austria, n = 4; Ireland, n = 4; UK, n = 4; Czech Republic, n = 3; Greece, n = 2; Finland, n = 2; Romania, n = 2; Croatia, n = 1; Norway, n = 1; Portugal, n = 1; Serbia, n = 1), 154 from North America (USA, n = 140; Canada, n = 14), 9 from South America (Brazil, n = 5; Argentina, n = 3; Mexico n = 1), 9 from Oceania (Australia, n = 8; New Zealand, n = 1), and 6 from Africa (Egypt, n = 2; Ethiopia, n = 1; Nigeria, n = 1; South Africa, n = 1; Tunisia, n = 1).
Findings
Table 1 reports the characteristics of the 565 included articles. Pediatric and non-pediatric studies did not significantly differ with respect to number of subjects per publication, study design, quartile of the journal, and country income setting. Information on the laboratory technique employed for sodium assessment was more commonly (p = 0.034) reported in pediatric (n = 15, 28%) than in non-pediatric (n = 81, 16%) reports.
Information on the technique employed for sodium assessment was obtained from the corresponding authors for further 175 reports. Indirect potentiometry was the most used technique (81%) among both pediatric and non-pediatric studies (Table 2). In children, however, a larger (p = 0.001) subset of studies was performed using the direct method (45% versus 15%).
The frequency of reports with and without information on the technique for sodium assessment was not different with respect to study design (observational vs interventional), the data collection (retrospective vs prospective), the quartile of the journal where the paper was published (first vs others), the number of included subjects (10–99 vs ≥ 100), the country income setting (high versus others), and the inclusion of neonates (yes vs no) among the 54 pediatric studies (Fig. 2).
Discussion
This is the first systematic analysis of the literature on the techniques employed to assess sodium in childhood. This study points out that more than about two-third of the papers dealing with hyponatremia in pediatrics do not report any information on the technique used to assess sodium. On the other hand, the information was more frequently available in pediatric than in non-pediatric studies. Moreover, indirect potentiometry was overall the most employed technique.
Most pediatric articles (> 70%) included in our analysis fail to report any information on the technique employed for sodium analysis, independently from the study characteristics (including the involvement of neonates), the journal quartile ranking, and the country income setting.
This widespread attitude suggests that the techniques are considered interchangeable by most pediatric (and non-pediatric) researchers. This speculation is also supported by the fact that even systematic reviews and meta-analyses about the effects of medical interventions on sodium levels include data without considering the technique used to measure this electrolyte in the original studies [7,8,9,10]. This choice has a potential high relevance, considering that some of these papers included also critically ill patients: in this setting, the discrepancy between the two methods is often clinically relevant, i.e., > 4 mmol/L [3, 11].
The findings obtained in the original papers or after inquiry about the technique used for sodium analysis deserve some further considerations. Interestingly, pediatric studies reported such information more often than non-pediatric studies (28% vs 16%, respectively). Moreover, although indirect potentiometry represents the most frequently used technique, the direct potentiometry is more frequently employed in pediatric (45%) than in non-pediatric studies (15%). Indirect potentiometry, which utilizes diluted blood samples, assumes that the non-watery fraction of plasma is ≈7% [12]. A reduction in lipid and protein concentration might result in an increase of the plasma watery fraction and, consequently, possible spuriously high sodium measurements [12, 13]. On the other hand, direct potentiometry, which utilizes undiluted blood samples, is unaffected by changes of the non-watery fraction of plasma [4]. Therefore, the use of direct potentiometry is especially important for sodium assessment in neonates and infants, who typically present with lower circulating levels of immunoglobulins, clotting and anti-clotting factors, and lipids [14, 15].
Sodium abnormalities in childhood might occur both at presentation and during hospitalization [16, 17]. Neurological injuries and death have been reported due to hospital acquired-dysnatremia [18, 19]. Therefore, sodium monitoring is essential, and the combination of different sampling and analytical techniques might result in suboptimal children management [20,21,22]. In addition, direct potentiometry requires small amount of blood and has a relevantly shorter turnaround time [6, 13]. Therefore, most international societies recommend that indirect technique is progressively abandoned [23, 24].
This study has some important implications. First, it points out that the possibility to compare or pool data from available literature dealing with hyponatremia is affected by the unavailability of information on the technique for sodium measurement. Second, a greater awareness among pediatric researchers on the potential discrepancies between the two techniques is needed. Third, our findings suggest that also pediatric hospitalists poorly consider the technique used for sodium assessment. Finally, we suggest that the description of the technique for sodium assessment should be included in every scientific report and considered to rate the study quality.
This analysis has at least two limitations. Firstly, it includes only papers with the term “hyponatremia” in the title. Therefore, it does not consider papers which assessed or monitored sodium levels as secondary outcomes. This strategy could have led to underestimate the issue of sodium measurement in the pediatric literature, since included papers had a special emphasis on sodium and might be more prone to report information on its measurement. Moreover, papers focusing on hypernatremia were not included. However, hypernatremia is nowadays rather rare in childhood [25, 26].
Conclusions
This analysis points out that most reports (adult papers more often than pediatric papers) do not report any information on the technique used to assess sodium and, when reported, indirect potentiometry is still the most popular technique. Although international authorities have recommended the implementation of direct potentiometry, a low awareness on this issue is still widespread in pediatric research.
Data availability
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References
Buffington MA, Abreo K (2016) Hyponatremia: a review. J Intensive Care Med 31:223–236. https://doi.org/10.1177/0885066614566794
Moritz ML, Ayus JC (2021) 0.9% saline and balance crystalloids in acute ill patients: trading one problem for another. J Crit Care 63:254–256. https://doi.org/10.1016/j.jcrc.2021.01.011
Dimeski G, Morgan TJ, Presneill JJ, Venkatesh B (2012) Disagreement between ion selective electrode direct and indirect sodium measurements: estimation of the problem in a tertiary referral hospital. J Crit Care 27:326.e9–16. https://doi.org/10.1016/j.jcrc.2011.11.003
Goldwasser P, Ayoub I, Barth RH (2015) Pseudohypernatremia and pseudohyponatremia: a linear correction. Nephrol Dial Transplant 30:252–257. https://doi.org/10.1093/ndt/gfu298
Milani GP, Edefonti V, De Santis R et al (2020) Disagreement between direct and indirect potentiometric Na+ determination in infancy and childhood. Clin Chem Lab Med 58:e117–e119. https://doi.org/10.1515/cclm-2019-0931
Malandrini S, Lava SAG, Bianchetti MG et al (2021) Which laboratory technique is used for the blood sodium analysis in clinical research? A systematic review. Clin Chem Lab Med 59:1501–1506. https://doi.org/10.1515/cclm-2021-0293
Hasim N, Bakar MAA, Islam MA (2021) efficacy and safety of isotonic and hypotonic intravenous maintenance fluids in hospitalised children: a systematic review and meta-analysis of randomised controlled trials. Children (Basel) 8:785. https://doi.org/10.3390/children8090785
McNab S, Ware RS, Neville KA et al (2014) Isotonic versus hypotonic solutions for maintenance intravenous fluid administration in children. Cochrane Database Syst Rev CD009457. https://doi.org/10.1002/14651858.CD009457.pub2
Foster BA, Tom D, Hill V (2014) Hypotonic versus isotonic fluids in hospitalized children: a systematic review and meta-analysis. J Pediatr 165:163–169.e2. https://doi.org/10.1016/j.jpeds.2014.01.040
Padua AP, Macaraya JRG, Dans LF, Anacleto FE (2015) Isotonic versus hypotonic saline solution for maintenance intravenous fluid therapy in children: a systematic review. Pediatr Nephrol 30:1163–1172. https://doi.org/10.1007/s00467-014-3033-y
Almeida HI, Mascarenhas MI, Loureiro HC et al (2015) The effect of NaCl 0.9% and NaCl 0.45% on sodium, chloride, and acid-base balance in a PICU population. J Pediatr (Rio J) 91:499–505. https://doi.org/10.1016/j.jped.2014.12.003
Lavagno C, Milani GP, Uestuener P et al (2017) Hyponatremia in children with acute respiratory infections: a reappraisal. Pediatr Pulmonol 52:962–967. https://doi.org/10.1002/ppul.23671
Lava SAG, Bianchetti MG, Milani GP (2017) Testing Na+ in blood. Clin Kidney J 10:147–148. https://doi.org/10.1093/ckj/sfw103
Adeli K, Higgins V, Nieuwesteeg M et al (2015) Biochemical marker reference values across pediatric, adult, and geriatric ages: establishment of robust pediatric and adult reference intervals on the basis of the Canadian Health Measures Survey. Clin Chem 61:1049–1062. https://doi.org/10.1373/clinchem.2015.240515
De Henauw S, Michels N, Vyncke K et al (2014) Blood lipids among young children in Europe: results from the European IDEFICS study. Int J Obes (Lond) 38(Suppl 2):S67-75. https://doi.org/10.1038/ijo.2014.137
Moritz ML, Ayus JC (2015) Maintenance intravenous fluids in acutely ill patients. N Engl J Med 373:1350–1360. https://doi.org/10.1056/NEJMra1412877
Easley D, Tillman E (2013) Hospital-acquired hyponatremia in pediatric patients: a review of the literature. J Pediatr Pharmacol Ther 18:105–111. https://doi.org/10.5863/1551-6776-18.2.105
Moritz ML, Ayus JC (2003) Prevention of hospital-acquired hyponatremia: a case for using isotonic saline. Pediatrics 111:227–230. https://doi.org/10.1542/peds.111.2.227
Santi M, Lava SAG, Camozzi P et al (2015) The great fluid debate: saline or so-called “balanced” salt solutions? Ital J Pediatr 41:47. https://doi.org/10.1186/s13052-015-0154-2
Loughrey CM, Hanna EV, McDonnell M, Archbold GP (2006) Sodium measurement: effects of differing sampling and analytical methods. Ann Clin Biochem 43:488–493. https://doi.org/10.1258/000456306778904560
King RI, Mackay RJ, Florkowski CM, Lynn AM (2013) Electrolytes in sick neonates - which sodium is the right answer? Arch Dis Child Fetal Neonatal Ed 98:F74–F76. https://doi.org/10.1136/archdischild-2011-300929
Bertini A, Milani GP, Simonetti GD et al (2016) Na+, K+, Cl−, acid–base or H2O homeostasis in children with urinary tract infections: a narrative review. Pediatr Nephrol 31:1403–1409. https://doi.org/10.1007/s00467-015-3273-5
Spasovski G, Vanholder R, Allolio B et al (2014) Clinical practice guideline on diagnosis and treatment of hyponatraemia. Intensive Care Med 40:320–331. https://doi.org/10.1007/s00134-014-3210-2
Burnett RW, Covington AK, Fogh-Andersen N et al (2000) Recommendations for measurement of and conventions for reporting sodium and potassium by ion-selective electrodes in undiluted serum, plasma or whole blood. International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). IFCC Scientific Division Working Group on Selective Electrodes. Clin Chem Lab Med 38:1065–1071. https://doi.org/10.1515/CCLM.2000.159
Mazzoni MB, Milani GP, Bernardi S et al (2019) Hyponatremia in infants with community-acquired infections on hospital admission. PLoS ONE 14:e0219299. https://doi.org/10.1371/journal.pone.0219299
Peruzzo M, Milani GP, Garzoni L et al (2010) Body fluids and salt metabolism - part II. Ital J Pediatr 36:78. https://doi.org/10.1186/1824-7288-36-78
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Open access funding provided by Università degli Studi di Milano within the CRUI-CARE Agreement. This study was (partially) funded by the Italian Ministry of Health-Current research IRCCS.
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AC, SM, MGB, GPM: conceptualization and study design. AC, SM: literature search and data extraction; MGB: data analysis; CA, MGB, GPM: data interpretation; BC, FM and PBF gave a significant contribution in their field of expertise. AC, GPM, and MGB wrote the first draft of the manuscript. All authors revised the first draft of the manuscript and approved the manuscript as submitted.
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Corsello, A., Malandrini, S., Bianchetti, M.G. et al. Sodium assessment in neonates, infants, and children: a systematic review. Eur J Pediatr 181, 3413–3419 (2022). https://doi.org/10.1007/s00431-022-04543-3
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DOI: https://doi.org/10.1007/s00431-022-04543-3