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Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone triggers mitochondrial dysfunction and apoptosis in neutrophils through calcium signaling

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Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that utilizes the quorum-sensing (QS) process to regulate the production of different virulence factors and biofilm. N-3-oxo-dodecanoyl-l-homoserine lactone (C12) is a key QS molecule of P. aeruginosa which interacts with the mammalian immune cells and modulates their function. Here, we investigated the molecular mechanism of C12-induced apoptosis in neutrophils. Our data show that C12 causes apoptosis in neutrophils through an elevation in cytosolic and mitochondrial Ca2+ levels. Besides, C12 induces phosphatidylserine (PS) exposure, mitochondrial membrane potential (MMP) depolarization, mitochondrial permeability transition pore (MPTP) formation and mitochondrial reactive oxygen species (mROS) generation. C12-induced rise in intracellular Ca2+ level is majorly contributed by endoplasmic reticulum store through the activation of inositol 1, 4, 5-triphosphate receptor. Intracellular calcium chelation inhibited C12-induced mitochondrial dysfunction and apoptosis. Further, inhibition of mitochondrial Ca2+ uniporter by ruthenium red or Ru360 abrogated C12-induced mitochondrial Ca2+ uptake, MMP loss, MPTP opening, mROS production, and PS exposure. These mechanistic insights are expected to provide a better understanding of the role of C12 in P. aeruginosa pathogenesis.

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Acknowledgements

This research work was supported by the Grant from the Department of Biotechnology (DBT) Ministry of Science and Technology (BT/PR6175/GBD/27/367/2012); Science and Engineering Research Board (SERB) (EMR/2014/000496) Government of India. The authors gratefully acknowledge the University Grant Commission (UGC), and the Ministry of Human Resource Development (MHRD) for enabling fellowship. Authors are thankful to the Centre for Interdisciplinary Research (CIR), MNNIT Allahabad for providing the facility of the fluorescence spectrophotometer. Authors are also thankful to the Central Instrumentation Facility (CIF), Department of Biotechnology MNNIT Allahabad for providing the flow cytometry facility.

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  1. Pradeep Kumar Singh and Vivek Kumar Yadav contributed equally to this work.

Authors and Affiliations

  1. Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Prayagraj, Uttar Pradesh, 211004, India

    Pradeep Kumar Singh, Vivek Kumar Yadav, Manmohit Kalia & Vishnu Agarwal

  2. Department of Mathematics, National Institute of Technology Raipur, Raipur, Chhattisgarh, 492010, India

    Deepmala Sharma

  3. Department of Reproductive Biology, All India Institute of Medical Sciences, New Delhi, 110029, India

    Deepak Pandey

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Contributions

PKS, VKY, and VA conceived and designed the experiments; PKS, VKY, and MK performed the experiments; PKS, VKY, VA, DP, and DS analyzed the data; VA provided comments and technical support; PKS, VKY, MK and VA wrote the paper.

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Correspondence to Vishnu Agarwal.

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Singh, P.K., Yadav, V.K., Kalia, M. et al. Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxo-dodecanoyl)-l-homoserine lactone triggers mitochondrial dysfunction and apoptosis in neutrophils through calcium signaling. Med Microbiol Immunol 208, 855–868 (2019). https://doi.org/10.1007/s00430-019-00631-8

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