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Specific detection of antibodies to different flaviviruses using a new immune complex ELISA

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Abstract

Immune complex (IC) ELISAs for IgG antibodies to various virus antigens have turned out to be both highly specific and sensitive. During incubation of a labelled antigen with the serum samples, ICs are formed, which bind to microtiter plates coated with rheumatoid factor (RF) IgM. Here, we describe an improved coating of the solid-phase support comparing various Fc-receptor molecules. IC ELISAs were applied to detect human IgG antibodies to the highly virus-specific ED3 domain of West Nile- and tick-borne encephalitis virus envelopes. Compared with other Fc-receptor molecules like RF or C1q, FcγRIIA (CD32) turned out to bind the ICs composed of IgG antibodies and peroxidase-labelled ED3 antigens more efficiently. Due to low background reactions, sera could be tested at a dilution of 1:10. Moreover, using CD32 instead of RF coating, anti-flavivirus antibodies could be detected in various animal species

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Acknowledgments

We would like to thank Dr. Ute Ziegler, Friedrich Loeffler Institute for providing rabbit and chicken sera with anti-WN antibodies, Prof. Jan ter Meulen for human anti-ED3 West Nile MAB and Dr. Strubel for anti-ED3 TBE mouse MABs. Moreover, the continuous excellent assistance of Michael Reinholz and Doris von Schassen will be thankfully acknowledged.

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Authors and Affiliations

  1. Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Bernhard-Nocht-Str.74, 20359, Hamburg, Germany

    Herbert Schmitz, Martin Gabriel & Petra Emmerich

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  1. Herbert Schmitz
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  2. Martin Gabriel
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  3. Petra Emmerich
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Correspondence to Herbert Schmitz.

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The experiments comply with the current laws of Germany.

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Schmitz, H., Gabriel, M. & Emmerich, P. Specific detection of antibodies to different flaviviruses using a new immune complex ELISA. Med Microbiol Immunol 200, 233–239 (2011). https://doi.org/10.1007/s00430-011-0195-0

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  • DOI: https://doi.org/10.1007/s00430-011-0195-0

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