Abstract
Limbic system-associated membrane protein (LSAMP) is a neural cell adhesion molecule involved in neurite formation and outgrowth. The purpose of the present study was to characterize the distribution of alternatively transcribed Lsamp isoforms in the mouse brain and its implications on the regulation of behavior. Limbic system-associated membrane protein 1b transcript was visualized by using a mouse strain expressing beta-galactosidase under the control of Lsamp 1b promoter. The distribution of Lsamp 1a transcript and summarized expression of the Lsamp transcripts was investigated by non-radioactive in situ RNA hybridization analysis. Cross-validation was performed by using radioactive in situ hybridization with oligonucleotide probes. Quantitative RT-PCR was used to study correlations between the expression of Lsamp isoforms and behavioral parameters. The expression pattern of two promoters differs remarkably from the developmental initiation at embryonic day 12.5. Limbic system-associated membrane protein 1a promoter is active in “classic” limbic structures where the hippocampus and amygdaloid area display the highest expression. Promoter 1b is mostly active in the thalamic sensory nuclei and cortical sensory areas, but also in areas that regulate stress and arousal. Higher levels of Lsamp 1a transcript had significant correlations with all of the measures indicating higher trait anxiety in the elevated plus-maze test. Limbic system-associated membrane protein transcript levels in the hippocampus and ventral striatum correlated with behavioral parameters in the social interaction test. The data are in line with decreased anxiety and alterations in social behavior in Lsamp-deficient mice. We propose that Lsamp is involved in emotional and social operating systems by complex regulation of two alternative promoters.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Limbic system-associated membrane protein (LSAMP) is a neural cell adhesion molecule expressed on the neuronal dendrites and somata (Zacco et al. 1990) on structures known to be especially important for emotional and motivational functions (Heimer and Van Hoesen 2006; Levitt 1984). Recently, LSAMP has been linked with a spectrum of psychiatric disorders in humans. The levels of the LSAMP protein have been found to be approximately 20 % increased in the postmortem frontal cortex both in patients with schizophrenia and bipolar disorder (Behan et al. 2009). Polymorphisms in the LSAMP gene have been associated with depression (Koido et al. 2012) and LSAMP has been suggested to have a role in the neurobiology of male completed suicide (Must et al. 2008).
Functional studies have shown that LSAMP can promote or inhibit neurite outgrowth depending on interactions with other members of the IgLON family (Mann et al. 1998; Gil et al. 2002), indicating its prominent role in neurite formation and synaptogenesis (Hashimoto et al. 2009). Before second postnatal week of development, LSAMP is transiently also expressed in developing axons and growth cones (Horton and Levitt 1988) indicating importance in developing of the brain structures. However, the lack of obvious deviations in brain organization in both of the two independently created Lsamp-deficient mouse strains (Catania et al. 2008; Innos et al. 2011) suggests that LSAMP is mediating finely specialized aspects of circuit formation and maturation of the limbic system. Genetic deletion of the Lsamp gene in mice induced no detectable changes in sensory and motor development, but caused increased activity in novel environments and reduced anxiety-like behavior in both knockout models (Catania et al. 2008; Innos et al. 2011). Increased trait anxiety in rats has been shown to be related with increased level of the Lsamp transcript in the amygdaloid area, periaqueductal gray (Nelovkov et al. 2006), raphe, hippocampus and frontal cortex (Alttoa et al. 2010). Elevated levels of the Lsamp transcript in the amygdaloid area of rats have been associated with acute fear reaction (Koks et al. 2004) and fear conditioning (Lamprecht et al. 2009). The amino acid sequence of LSAMP is highly conserved among species. There is 99 % sequence identity between human and rodent LSAMP (Pimenta and Levitt 2004) and 91 % identity with chicken (Brümmendorf et al. 1997), indicating remarkable phylogenetic conservation of protein structure and associated functional properties. Growing evidence indicates that LSAMP is involved in the formation of anatomical substrate for emotional behavior both in rodents and humans.
Pimenta and Levitt (2004) reported revised genomic structure of the mouse Lsamp gene, demonstrating that, besides the well-known exon 1 (now referred as exon 1b), the Lsamp gene has an alternative exon 1 (currently exon 1a) located 1.6 Mbp upstream, and both of them have separate promoter sequences. The two-promoter structure is conserved and has been described in mouse, rat, human (Pimenta and Levitt 2004) and also in chicken (Brümmendorf et al. 1997). Limbic system-associated membrane protein distribution in the whole adult mammalian brain was originally described in rat using immunohistochemistry (Levitt 1984) and in situ hybridization (Reinoso et al. 1996). The anatomical distribution of the LSAMP transcript and protein has been extensively described in various species (Chesselet et al. 1991; Côté et al. 1995; Prensa et al. 2003; Yamamoto and Reiner 2005), but the anatomical distribution of the alternative transcripts has not been reported. The purpose of the present study was to characterize the distribution of the two alternatively transcribed Lsamp isoforms in the mouse brain and to provide an initial analysis of the developmental activation of Lsamp promoters. Additionally, we investigated the relation between Lsamp expression and the regulation of emotional and social behavior.
Methods
Non-radioactive in situ RNA hybridization analysis with digoxigenin-UTP
Mouse Lsamp cDNA fragments were cloned from a cDNA pool from C57BL/6 mouse hippocampus and inserted into pGEM7-Zf(+) vector (Promega) to create an in situ probe. cDNA fragment specific for 1a promoter (400 bp) consisted of 1a-specific 5′UTR, exon 1a and exon 1a′. Universal Lsamp probe (567 bp) consisted of a cDNA fragment consisting of exons 2–6. RNA in situ hybridization on free-floating PFA-fixed 40 μm mouse brain cryosections using digoxigenin-UTP (Roche) labeled Lsamp sense and antisense RNA probes was performed as described previously (Braissant and Wahli 1998). As a major modification, active DEPC treatment was avoided and 0.25 % Triton X-100 was added to the PBS to improve probe penetration.
Radioactive in situ hybridization with oligonucleotides
Antisense 40′mer DNA oligonucleotide probes complementary to mouse Lsamp gene (Accession No. uc007zfr.1, UCSC Genome Browser, genome.ucsc.edu) Lsamp1a (5′-accccagcacccagacgctgtgcagccagtaggtcctcat-3′), Lsamp1b (5′-gaagaaggcagagcagtctcagtaggaccagcggcaactg-3′) and LsampUNI (5′-agagcatggcgcttctccagctcaacccgagggtccagag-3′) were labeled with 33P-dUTP by use of terminal transferase (Sigma-Aldrich, Europa). Free-floating in situ hybridization was carried out essentially as described previously (Hundahl et al. 2010).
Lsamp-deficient mice with beta-galactosidase knock-in
Detailed description of the generation of the Lsamp-deficient mice with a LacZ transgene can be found in Innos et al. (2011). Briefly, exon 1b of mouse Lsamp gene was replaced by an in-frame NLSLacZNeo cassette resulting in insertion of gene encoding beta-galactosidase immediately after Lsamp 1b promoter. X-Gal staining for detecting the distribution of 1b promoter specifically produced beta-galactosidase and NeuN immunostaining was performed as described previously (Luuk et al. 2008). The brains were cut into 100 μm coronal sections and transferred to glass slides. The contours in Fig. 4c, f and abbreviations in all the figures representing anatomical data have been adopted from the mouse brain atlas (Franklin and Paxinos 1997). For embryonic brains, the embryos were dissected from timed matings as in Philips et al. (2009). E13.5 (embryonic day 13.5) and E15.5 brains and/or embryos were fixed in 4 % PFA/PBS at 4 °C overnight for in situ hybridization, or for 30 min for X-gal staining. For cutting 50-μm vibratome sections, the stained (E13.5 and E15.5) specimens were inserted into 1 ml of 0.5 % gelatine/30 % BSA/20 % sucrose/PBS, wherein 140 μl of 25 % glutaraldehyde was added immediately before insertion and incubated for 10 min. The sections were mounted into 70 % glycerol and microphotographed.
Experimental animals for behavioral studies and quantitative real-time PCR analysis
Male C57BL/6 strain mice were used for all behavioral studies. In the first part of the behavioral studies we investigated the correlations between general behavioral profile and Lsamp expression that was measured 10 days after behavioral testing. All mice (n = 15) were subjected to a battery of three behavioral tests: elevated plus-maze test (day 0), locomotor activity test (day 5), and social interaction test (day 10), with less invasive tests preceding more stressful tests. Testing began at age 60 days (2 months). The motility box was carried out as described previously (Innos et al. 2013) and four behavioral parameters were recorded: (1) time spent moving in seconds (move, s); (2) distance travelled in meters (distance, m); (3) time spent in the center (time center, s); and (4) time spent in the corners (time corner, s). The plus-maze experiment was performed as described in Philips et al. (2010) and eight behavioral parameters were recorded: (1) the number of closed arm entries; (2) the number of open arm entries; (3) the ratio between open and closed arm entries; (4) the latency to enter open arm (latency, s); (5) time spent on open arms; (6) the number of protected head-dips; (7) the number of unprotected head-dips; and (8) the number of stretch-attend postures (SAPs). The social interaction test was carried out as described previously (Innos et al. 2012), briefly: 14 mice were matched into 7 pairs of two unfamiliar mice according to the bodyweight and (1) the time spent sniffing the partner’s anogenital area (anogenital sniffing, s) and (2) the time spent sniffing other body regions (sniffing other body parts, s) were recorded separately. These two measures were also summarized for (3) the time of total social sniffing for each animal (time of social sniffing, s). The animals were decapitated 10 days after the last experiment and three parts were dissected from the brains: the ventral striatum (including nucleus accumbens and olfactory tuberculi), hippocampus and temporal lobe (including temporal cortex and lateral, central and medial nuclei of amygdala).
In the second part of the behavioral studies, we investigated the influence of acute fear reaction on the expressional activity of the Lsamp gene. In order to induce acute fear reaction, fear conditioning was carried out by means of a computer-controlled Multi Conditioning System (TSE). Sixteen mice were divided into three groups: “Naïve” (n = 5); “Pre-conditioning” (n = 5) and “Conditioned fear” (n = 6). Training for the “Conditioned fear” and “Pre-conditioning” groups was performed in a dimly illuminated (15 lx) acrylic cage (30 × 30 × 30 cm) with stainless steel rod floor. Between subjects the cages were cleaned with isopropanol. On the first day after 150-s acclimation period animals received six trials with the following stimuli: 15 s tone (12 kHz; 70 dB) and bright light (pulsing at 200 ms) were terminated by a 2-s electric shock (0.6 mA) during which the light was constant. Inter-trial interval was 120 s (±50 %). After the last trial the animals were returned to their home cages. On the second day, animals in the “Conditioned fear” group were placed into the conditioning cages and exposed to similar stimuli without an electric shock for about 45 min (20 trials). The animals were killed immediately afterwards. We chose a 45-min duration as it has been shown that neuronal stimulation induces gene expression between timepoints 30–60 min (Lamprecht et al. 2009). “Naïve” and “Pre-conditioning” groups received no treatment on the second day. The hippocampus and temporal lobe (including the temporal cortex and the lateral, basolateral, central and medial nuclei of the amygdala) were dissected from the brains.
All the experiments were performed in accordance with the EU guidelines directive 86/609/EEC) and permit (No. 59, September 5, 2006) from the Estonian National Board of Animal Experiments.
qRT-PCR analysis of Lsamp expression in mouse brain areas
The tissue samples were frozen in liquid nitrogen. Dissection of the mouse brain was performed according to coordinates obtained from the mouse brain atlas (Franklin and Paxinos 1997). Limbic system-associated membrane protein mRNA level was determined by quantitative real-time PCR (qRT-PCR). Total RNA was extracted individually from each brain structure by using Trizol® reagent (Invitrogen, USA) according to the manufacturer’s protocol. First strand cDNA was synthesized by using Random Hexamers (Applied Biosystems) and SuperScript™ III Reverse Transcriptase (Invitrogen, USA). TaqMan Assay was designed for the detection of 1a- and 1b-specific transcripts. FAM-MGB-probe AACCGAGGCACGGACAAC was used with universal reverse primer combined with alternative forward oligos specific for either 1a isoform or 1b isoform. For mRNA quantification of the immediate-early gene c-fos, a Taqman assay from Applied Biosystems was employed (Mm00487425_m1). The detailed sequences of primers and probe for housekeeping gene hypoxanthine guanine phosphoribosyl transferase (Hprt-1) have been described in Areda et al. (2005). TaqMan® Universal PCR Master Mix was used in the ABI Prism 7900HT Sequence Detection System (Applied Biosystems, USA). Reactions were carried out in 10-μl reaction volumes in four replicates.
Data analysis
The analysis of qRT-PCR data was performed as described earlier (Raud et al. 2009). Briefly, qRT-PCR data in Fig. 3 is presented in linear scale, calculated as 2−ΔCT, where ΔCT is the difference in cycle threshold (CT) between the target gene (Lsamp) and housekeeper gene Hprt-1 (VIC-MGB). All data were analyzed using Statistica version 8.0 (StatSoft, Inc., USA). As the behavioral scores were not normally distributed, Spearman’s rank-order method was used for the calculation of correlation coefficients. In the fear conditioning study, one-way ANOVA (conditioning type as grouping variable) was performed. Tukey HSD post hoc analysis was used when applicable after statistically significant ANOVA. Data are presented as mean ± SEM.
Results
Lsamp 1a promoter activity predominates in “classic” limbic structures
Limbic system-associated membrane protein 1a transcript is intensively and specifically expressed in the brain areas that are commonly considered to be limbic structures (Heimer and Van Hoesen 2006; Morgane et al. 2005). Transcript 1a-specific staining is pronounced in the cingulate cortex (Cg, Fig. 2b, f; supplementary Fig. S1e), insular cortex (Ins, Fig. 2b, f; Fig. S1e, f), prelimbic cortex (PrL, Fig. S1e) and infralimbic cortex (IL, Fig. S1e). Extensive 1a-specific staining can be seen in the hippocampal formation (CA1, CA3 and DG; Figs. 1e–h, 2j), amygdalohippocampal area (AHi, Fig. 1g, h), lateral amygdaloid nucleus (La, Figs. 1e–g, 2j, 4c), basolateral (BL, Fig. 1e–h and BLA, Fig. 4c) and basomedial (BM, Fig. 1e, f) amygdaloid nuclei, medial amygdaloid nucleus (Me, Fig. 1f) and posterolateral (PLCo, Fig. 1e–g) and posteromedial (PMCo, Fig. 1g, h) cortical amygdaloid nuclei. Transcript 1b-specific X-Gal staining and in situ signal are much weaker in these areas. However, there is moderate 1b-specific staining in the central amygdaloid nucleus (Ce, Figs. 1i, 2l, 4f) and cortical amygdaloid nuclei (PLCo/PMCo, Fig. 1j–l). Expression of 1b isoform in the hippocampal formation is moderate and homogeneous (Figs. 1i–l, 2k, l; Fig. S1m). qRT-PCR results confirm the prevalent expression of 1a transcript in the hippocampal area and temporal lobe (Fig. 4e).
Lsamp 1b promoter activity is prevalent in the sensory nuclei and primary cortex areas
Many of the sensory systems are distinguished by 1b promoter-specific staining. In the major afferent pathways for somatosensory information, intense Lsamp 1b-specific staining is seen in the ventral posterior lateral thalamic nucleus (VPL; Figs. 1i–k, 2k, l) and primary somatosensory cortex (S1, Figs. 1j, k, 2c, d, g, k). The expression signal is the highest in the barrel field (S1b, f, Figs. 1i, 2h, l). There is moderate 1b transcript-specific staining in the gracile, cuneate (Cu, supplementary Fig. S1s) and spinal trigeminal (Sp5, Fig. S1s) nuclei and strong staining in the laterodorsal (Bezdudnaya and Keller 2008) thalamic nucleus (LD, Fig. 1i). In the ascending auditory pathway there is strong 1b-specific staining in the dorsal and ventral cochlear nuclei (DC and VC, respectively, Fig. S1o, p) and moderate 1b-specific staining in the superior olivary complex (SOC, Fig. S1p) and trapezoid body (Tz, Fig. S1p). Isoform 1b staining is strong in the nuclei of lateral lemniscus (LL, Fig. S1n), in the inferior colliculus (IC, Fig. S1o), in the medial geniculate nucleus (MG, Fig. 1l) and also in the primary and secondary (Au1/Au2, Fig. 1k, l) auditory cortex. In the visual pathway there is intensive 1b transcript-specific staining in the dorsal lateral geniculate thalamic nucleus (DLG, Fig. 1j, k; Fig. S1m) and primary visual cortex (V1, Fig. 1l; Fig. S1n). Strong 1b-specific X-Gal staining is also found in other brain areas receiving major projections from the retina: the superior colliculus (SC, Fig. S1n) and suprachiasmatic nucleus (SCh, Fig. S1i, j, l), and weak in the ventral lateral geniculate thalamic nucleus (VLG, Fig. 1j; Fig. S1m). In the sensory areas of the cortex, Lsamp 1b staining forms two distinct lines corresponding to layers 4 and 6 of the cortex (Lein et al. 2007) as estimated by comparing X-Gal staining (Fig. S1d) with NeuN immunoreactivity in the cortex (Fig. S1c). Staining reflecting promoter 1a activity is weak in the sensory areas of the cortex (Fig. S1a), and summarized expression of both isoforms reveals two moderate but distinct lines (Fig. S1b).
Both 1a and 1b promoters are expressed in brain areas involved in the processing of gustatory and olfactory information. In the gustatory system, 1b-specific staining is strong in the ventral posteromedial nucleus (VPM, Fig. 1i–k) and weak in the solitary nucleus (Sol, supplementary Fig. S1s). In the insular cortex only 1a isoform is expressed (Ins, Fig. 2b, f; Fig. S1e, f). In the olfactory system, the activity of 1b promoter is remarkable in the mediodorsal (Tham et al. 2009) thalamic nucleus (MD, Figs. 1i, 2k, l), and prevalent in the olfactory bulb (data not shown) and entorhinal cortex (Ent, Fig. S1n). The expression of 1a promoter is distinct in the nucleus of the lateral olfactory tract (LOT, Fig. S1k), and it dominates over 1b signal in the olfactory tubercle (Tu, Fig. 2b) and piriform cortex (Pir, Fig. 1e–h).
Differences in the activity of Lsamp 1a and 1b promoters in adult brain
Thalamic and hypothalamic nuclei are distinguished by the isoform-specific expression of Lsamp. Only two hypothalamic nuclei display activity for both 1a and 1b promoters: the paraventricular nucleus (Pa, supplementary Fig. S1k, l) and mammillary bodies (MB, Fig. 1c, g, k). High expression of 1a isoform is seen in the ventromedial hypothalamic nucleus, namely in the ventrolateral (VMHVL, Fig. 1e) and dorsomedial parts (VMHDM, Fig. 1e); weak 1b-specific staining is present in the anterior part of the VMH (Fig. 2k). Promoter 1a is active in the dorsomedial hypothalamic nucleus (DM, Fig. 1f), while the activity of 1b promoter is limited to the compact part of the DM (DMC, Fig. 2l). Strong 1a promoter-specific expression can be seen in the medial preoptic area (MPA, Fig. 2b), including medial preoptic nucleus, and also in the ventromedial preoptic nucleus (VMPO, Fig. S1f). Promoter 1a-specific staining is moderate in the anterolateral (LA, Fig. S1k) and lateral hypothalamus (LH, Fig. 1f, g; Fig. S1k, l). Strong 1b promoter-specific expression can be detected in the periventricular hypothalamic nucleus (Pe, Fig. 1i; Fig. S1l), anteroventral periventricular nucleus (AVPe, Fig. 2h), suprachiasmatic nucleus (SCh, Fig. S1i, j, l), supraoptic nucleus (SO, Fig. S1l) and arcuate nucleus (Arc, Fig. 1j, k).
Promoter 1a is specifically active in the anterior thalamus: the anteroventral thalamic nucleus (AV, supplementary Fig. S1g), reticular thalamic nucleus (Rt, Fig. S1k) and central medial thalamic nucleus (CM, Fig. 1e). Weak 1a-specific expression can be detected in the anteromedial thalamic nucleus (AM, Fig. S1g). Isoform 1b-specific staining can be seen in the sensory thalamic nuclei as described above, but also in the posterior thalamic nuclei (Po, Fig. 1i, j), lateral habenular nucleus (LHb, Fig. 1i), lateral posterior thalamic nucleus (LP, Fig. 1j, k), paratenial thalamic nucleus (PT, Fig. S1h) and reuniens thalamic nucleus (Re, Fig. S1l). There are numerous thalamic nuclei where both 1a and 1b promoters are active: the anterodorsal thalamic nucleus (AD, Fig. S1g, h), paraventricular thalamic nucleus (PV, Fig. S1k, l), paracentral thalamic nucleus (PC, Fig. S1g, h), parafascicular thalamic nucleus (PF, Fig. 1b, f, j), medial habenular nucleus (Mhb, Fig. 1a, e, i) and subthalamic nucleus (STh, Fig. 1b, f, j).
Alternative expression of Lsamp 1a and 1b promoters can be seen throughout the brain. Both 1a and 1b promoters are active in the dorsal (LSD, Fig. 2e–h) and ventral (LSV, Fig. 2e–h) part of the lateral septal nucleus, septofimbrial nucleus (SFi, Fig. 2f–h), subfornical organ (SFO, Fig. S1g, h) and retrosplenial granular cortex (RSG, Fig. 1e–g, i–k), whereas only 1b promoter is active in the retrosplenial agranular cortex (RSA, Fig. 1i–k). On the level of anterior commissure, 1b promoter is active in the bed nucleus of anterior commissure (BAC, Fig. 2h); 1a transcript is prevalent in the bed nuclei of stria terminalis (BST, Fig. 2f, Fig. S1f) and in the core of the nucleus accumbens (AcbC, Fig. 2b). Isoform 1b-specific staining is present in the dorsal peduncular cortex (DP, Fig. 2d), caudate putamen (CPu, Fig. 1j), dorsal endopiriform nucleus (Den, Fig. 1i–k), claustrum (Cl, Fig. 2h) and lateral stripe of striatum (LSS, Fig. 2h). The expression of the Lsamp transcript in the cerebellum is mostly initiated from promoter 1b, which is abundant in the Purkinje cell layer (Pc, Fig. S1r). There is moderate 1b expression in the molecular layer (Mc, Fig. S1r) and weak 1a expression in the granule cells (Gc, Fig. S1q). Limbic system-associated membrane protein expression is moderate in the spinal cord: both 1a (Fig. S1t) and 1b (Fig. S1u) isoforms are expressed in the ventral and dorsal horns. A detailed overview of the estimated intensities of Lsamp 1a, 1b and summarized transcripts in different brain areas has been presented in supplementary Table S2.
Differences in the activity of Lsamp 1a and 1b promoters in embryonic brain
We detected the first signals for both promoters of the Lsamp gene at around E12.5. Limbic system-associated membrane protein 1a transcript is firstly activated in the midbrain, being prominent in the outer layers of the neural tube (Fig. 3a, b), in the forebrain the first signs were detected at around E13.5 also in the outer surface of the neuroepithelium. The first signs of 1b transcript expression were detected in the lateral side of the lateral ventricle (Fig. 3d, e). During later embryonic development (E15.5), strong signal is detectable also in the lining of the aqueduct and in the deepest layers of the sensory region of the neocortex (S1, Fig. 3f). At E15.5, Lsamp 1a promoter is especially active in the caudate putamen (CPu, Fig. 3c), whereas this activity shades off during the first postnatal week (data not shown) and is not detectable in adult brain (CPu, Fig. 1f). The expression of Lsamp 1b promoter in the CPu is weak during development [as shown in E15,5; CPu (Fig. 3f) and moderate in adulthood (CPu, Fig. 1j)].
Expression of Lsamp transcripts correlates with behavioral measures of trait anxiety and social interaction
We looked for the correlations between various behavioral parameters and the activity of Lsamp transcript expression in three brain areas (ventral striatum, hippocampus and temporal lobe). Most of the significant correlations emerged between Lsamp expression in the temporal cortex and behavioral parameters in the elevated plus maze (Table 1). Both Lsamp 1a and 1b transcript levels were negatively correlated with time on open arms in the elevated plus maze (Fig. 4a). Furthermore, Lsamp 1a transcript levels were negatively correlated with unprotected headdips in the elevated plus maze (Fig. 4b), with the number of open arm entries and with the ratio between open and closed arm entries. There was also a significant positive correlation between Lsamp 1a transcript levels and the latency to enter open arm. Additionally, Lsamp transcript levels in the hippocampus and ventral striatum correlated with behavioral parameters in the social interaction test. Both Lsamp 1a and 1b transcript levels were negatively correlated with the time that the mice spent sniffing the other animal. The time of anogenital sniffing positively correlated with Lsamp 1a activity in the ventral striatum.
In the experiment for acute fear response, all the mice in the “Conditioned fear” group displayed an obvious fear reaction as evidenced by startling response and freezing (data not shown). The “Conditioned fear” group had significantly higher c-Fos expression in the amygdala [F (2,13) = 11.6, p < 0.01] and hippocampus [F (2,13) = 8.8, p < 0.01] than the “Naïve” or “Pre-conditioning” groups (Fig. 3d). Furthermore, in the “Conditioned fear” group, the c-Fos activation in the temporal lobe was significantly higher than in the hippocampus (p < 0.05), indicating specific activation of the amygdaloid area in response to conditioned fear. There were no statistically significant changes in the expression levels of Lsamp 1a of 1b transcripts at the 45 min timepoint (Fig. 3e).
Discussion
Limbic system-associated membrane protein has been linked with a wide spectrum of psychiatric disorders in humans and with behavioral alterations in rodents. The limbic system-specific neuroanatomical distribution of the Lsamp transcript and protein has been described in various species (Chesselet et al. 1991; Côté et al. 1996; Reinoso et al. 1996; Yamamoto and Reiner 2005) and LSAMP immunostaining has been used as an anatomical marker of the limbic system (Prensa et al. 2003; Uroz et al. 2004). Here we provide first evidence that the activity profile of the two alternative promoters of the Lsamp gene has a heterogenous anatomical distribution in the developing and adult brain and the activity of these two promoters correlates with trait anxiety and social behavior in mice.
Limbic system-associated membrane protein 1a promoter is transcriptionally active in the “classic” limbic structures known to be especially important for emotional and motivational functions (Heimer and Van Hoesen 2006). Namely, 1a promoter is specifically active in the hippocampal formation, temporal cortex and amygdaloid area and also in the ventral striatum that includes nucleus accumbens and olfactory tuberculi; furthermore, 1a transcript is expressed specifically in the limbic, cingulate and insular cortex. Promoter 1a is active in the anterior thalamic nuclei that have been specified as “limbic thalamus” (Vogt and Gabriel 1993; Marchand et al. 2013) and in the anterior hypothalamus, including preoptic area that is a major interacting structure of the limbic system (Morgane et al. 2005). Promoter 1b of the Lsamp gene is notably active in the sensory pathways ranging from the brainstem and sensory nuclei in the thalamus and up to the primary sensory areas in the cortex. In the cerebral cortex, the signal from 1b promoter can be seen in layers 4 and 6 of the cortex (supplementary Fig. S1d) emphasizing systematic expression in the areas involved in the processing of sensory input. Layer 4 is the primary recipient of sensory input from the thalamus (Liao and Lee 2012) and most thalamic relay neurons receive feedback from layer 6 of the same cortical column they innervate (Lam and Sherman 2010). The specific activity of 1b promoter is obvious in the sensory pathways in visual, auditory and somatosensory areas. However, both 1a and 1b promoters are active in the neural pathways transmitting olfactory and gustatory information. This finding can be anticipated as brain regions associated with olfactory and gustatory perception (e.g., the piriform cortex and insular cortex) are often overlapping with brain regions that are involved in emotional processing (Gutman et al. 2013).
While Lsamp 1b promoter is predominantly active in sensory areas, it is also highly expressed in areas that are either traditional components of the limbic system and/or actively involved in regulating stress and arousal, such as the mammillary bodies and the paraventricular nucleus of the hypothalamus. Additionally, 1b promoter is prevalently active in the central nucleus of amygdala that is commonly referred to as the central part of the limbic structures (Heimer and Van Hoesen 2006). However, according to a recent study, the central nucleus of amygdala gets projections from several sensory-related regions (Bienkowski and Rinaman 2013). 1b promoter activity is highly enriched but not strictly limited to sensory areas; however, connections with the sensory systems can be found in most of the areas expressing 1b transcript (see supplementary Table S2 for overview). The expression of Lsamp in the brain areas processing sensory information has been reported in earlier studies (Reinoso et al. 1996; Yamamoto and Reiner 2005). Yet, the discussion of whether the distribution of LSAMP is really specific for limbic structures has not been raised. Our results indicate that it is questionable to use the summarized LSAMP staining as a marker of the limbic regions, however, we propose that Lsamp 1a transcript is intensively and specifically expressed in the brain areas that are commonly considered to be limbic structures (Heimer and Van Hoesen 2006; Morgane et al. 2005; Kötter and Stephan 1997).
Developmentally, the initial activation of both 1a and 1b promoters can be detected on embryonic day E12.5, the time preceding active neurite outgrowth and path-finding, suggesting that Lsamp is important already at the time of neuroepithelial patterning. The expressional initiation is consistent with previously published data about the developing brain of the rat (Pimenta et al. 1996). However, as in the adult, the expression pattern of the two alternative promoters differs remarkably at this early stage. Limbic system-associated membrane protein 1a promoter activation is initiated in the outer surface of the neuroepithelium. The first signs of 1b transcript expression, on the other hand, are detected in the lateral aspect of the lateral ventricle and in the lining of the aqueduct. According to our primary analysis, the extensive 1a transcript-specific staining in the dorsal striatum that disappears during the first postnatal week represents the most striking difference in the distribution of Lsamp between the developing and the adult brain. Strong 1a staining in the dorsal striatum before the second postnatal week overlaps with the time period of transient expression of LSAMP on developing axons (Horton and Levitt 1988) suggesting that Lsamp 1a promoter is specifically activated during the regulation of striatal development. The expression patterns of the Lsamp 1a and 1b transcripts during mouse embryogenesis indicate a potential role of the Lsamp gene and protein in the development all over the brain.
Increased levels of the Lsamp transcript have been associated with lower activity and higher levels of trait anxiety or acute fear reaction and the genetic deletion of the Lsamp gene in mice resulted in increased activity in novel environments and reduced anxiety (Catania et al. 2008; Innos et al. 2011). To get further insight of how LSAMP is involved in the regulation of adaptive and emotional behavior by the usage of alternative promoters, we studied behavioral correlates for Lsamp 1a and 1b transcripts in three brain areas. Most of the significant correlations appeared between Lsamp expression in the temporal lobe and behavioral parameters in the elevated plus maze. Higher levels of Lsamp 1a transcript had significant correlations with all of the measures indicating higher anxiety (Cruz et al. 1994) in the elevated plus-maze test. Higher levels of Lsamp 1b in the temporal cortex correlated significantly with the time that mice spent on open arm that is again, a common measure of anxiety. Current results are correlative in nature, but well in line with our previous loss-of-function studies with Lsamp-deficient mice displaying decreased anxiety (Innos et al. 2011).
In the current study, we did not detect any expressional changes in Lsamp transcripts after acute fear reaction although there is evidence that Lsamp is also activated in reaction to acute fear in the amygdaloid area of rats (Koks et al. 2004) and in the lateral amygdaloid nucleus of rats after fear conditioning (Lamprecht et al. 2009). It is most likely that acute changes are limited to specific subnuclei in the amygdala that can be more exactly separated from rat brain. In our study, we used the temporal lobe of mice, including all the amygdaloid nuclei and also the temporal cortex. However, the expression of Lsamp transcripts of the same area was significantly correlated with trait anxiety of the mice. We provide further evidence that the Lsamp gene is implicated in the formation of fear and anxiety processing circuits in the temporal cortex/amygdaloid area (Nieh et al. 2012), but this influence seems to be mediated differentially in acute fear reaction and trait anxiety. Although related, fear and trait anxiety are distinctly different—fear is an emotional reaction triggered by an immediate threat, while anxiety is a state of heightened apprehension in the absence of an immediate threat (Davis et al. 2010). Taken together, our results fit with previous evidence relating increased levels of Lsamp with heightened trait anxiety (Nelovkov et al. 2006; Alttoa et al. 2010); the implication of Lsamp in acute fear reaction seems to be more complicated and might be related with certain subnuclei in the amygdala or/and specific time points.
Both Lsamp 1a and 1b transcript levels in the hippocampus correlated negatively with social sniffing and Lsamp 1a transcript in the ventral striatum was positively correlated with the time of anogenital sniffing in the social interaction test. The implication of LSAMP in the regulation of social activity is again in line with the behavioral phenotype of Lsamp-deficient mice displaying lack of inter-male dominance hierarchy and whisker trimming (Innos et al. 2011). The positive correlation of Lsamp 1a transcript with anogenital sniffing fits with reduced anogenital sniffing accompanying reduced inter-male aggressiveness reported in Lsamp-deficient mice (Innos et al. 2011). General social sniffing is not altered in Lsamp-deficient mice; therefore, the correlation between higher level of Lsamp in the hippocampal area and shorter time of social sniffing may reflect higher Lsamp levels correlating with higher trait anxiety as the social interaction test was initially designed to measure anxiogenic and anxiolytic drug effects (File and Hyde 1978). Current data are in line with our previous reports showing that Lsamp-deficient mice have decreased anxiety and alterations in social behavior. Our results provide further evidence that Lsamp is functional in brain areas processing emotional reactions, particularly those related to anxiety/hyperactivity and social behavior.
Comparison of anatomical data from different species reveals high levels of conservation in the anatomical distribution of LSAMP transcript/protein. The summarized anatomical distribution from the current study is in line with the data from humans and primates: in humans LSAMP expression is intensive in the paraventricular thalamic nucleus (Uroz et al. 2004) and moderate in the nucleus accumbens and claustrum (Prensa et al. 2003). In primates, the hippocampus displays the strongest immunoreactivity, amygdala has a highly heterogeneous staining pattern (Côté et al. 1996) and ventral striatum displays more intense LSAMP immunostaining than the dorsal striatum (Côté et al. 1995). Furthermore, the twin promoter structure of the LSAMP gene seems to be essential also in humans. In the human genome, the exon 1a′ has been mutated by insertion of two nucleotides introducing a frame shift and resulting in a termination codon. Surprisingly, consequent loss of the acceptor site prevents the inclusion of the mutated exon 1a′ (Pimenta and Levitt 2004). The consequence of these two evolutionary events suggests that two promoters and alternatively regulated expression is needed for functional emotional responses in humans. Altered expression of LSAMP has been demonstrated in brain areas of human psychiatric patients in the frontal cortex (Behan et al. 2009) and hippocampus (Hyde et al. 1997), but in the previous studies 1a and 1b transcripts of the LSAMP gene in the nervous system have not been distinguished. Certain SNPs that reside in the first intron flanking exon 1b of LSAMP are associated with major depressive disorder (Koido et al. 2012). Furthermore, lower expression level of LSAMP 1a transcript has been linked with the susceptibility allele for coronary artery disease (Vance 2007). These data emphasize the importance of studying 1a and 1b isoforms separately to find out relevant information that can be used in diagnostic panels in the future.
The distinct system-specific use of alternative promoters reveals highly organized transcriptional regulation of LSAMP gene/protein associated with a broad spectrum of emotional behaviors. We propose that LSAMP is involved in emotional and social operating systems by complex regulation of two alternative promoters that guide the development of neural circuits in the limbic and sensory brain areas.
References
Alttoa A, Kõiv K, Hinsley TA, Brass A, Harro J (2010) Differential gene expression in a rat model of depression based on persistent differences in exploratory activity. Eur Neuropsychopharmacol 20(5):288–300
Areda T, Kõks S, Philips MA, Vasar E, Karis A, Asser T (2005) Alterations in opioid system of the rat brain after cat odor exposure. Neurosci Lett 377(2):136–139
Behan AT, Byrne C, Dunn MJ, Cagney G, Cotter DR (2009) Proteomic analysis of membrane microdomain-associated proteins in the dorsolateral prefrontal cortex in schizophrenia and bipolar disorder reveals alterations in LAMP, STXBP1 and BASP1 protein expression. Mol Psychiatry 14(6):601–613
Bezdudnaya T, Keller A (2008) Laterodorsal nucleus of the thalamus: a processor of somatosensory inputs. J Comp Neurol 507(6):1979–1989
Bienkowski MS, Rinaman L (2013) Common and distinct neural inputs to the medial central nucleus of the amygdala and anterior ventrolateral bed nucleus of stria terminalis in rats. Brain Struct Funct 218(1):187–208
Braissant O, Wahli W (1998) Differential expression of peroxisome proliferator-activated receptor-alpha, -beta, and -gamma during rat embryonic development. Endocrinology 139(6):2748–2754
Brümmendorf T, Spaltmann F, Treubert U (1997) Cloning and characterization of a neural cell recognition molecule on axons of the retinotectal system and spinal cord. Eur J Neurosci 9(6):1105–1116
Catania EH, Pimenta A, Levitt P (2008) Genetic deletion of Lsamp causes exaggerated behavioral activation in novel environments. Behav Brain Res 188(2):380–390
Chesselet MF, Gonzales C, Levitt P (1991) Heterogeneous distribution of the limbic system-associated membrane protein in the caudate nucleus and substantia nigra of the cat. Neuroscience 40(3):725–733
Côté PY, Levitt P, Parent A (1995) Distribution of limbic system-associated membrane protein immunoreactivity in primate basal ganglia. Neuroscience 69(1):71–81
Côté PY, Levitt P, Parent A (1996) Limbic system-associated membrane protein (LAMP) in primate amygdala and hippocampus. Hippocampus 6(5):483–494
Cruz AP, Frei F, Graeff FG (1994) Ethopharmacological analysis of rat behavior on the elevated plus-maze. Pharmacol Biochem Behav 49(1):171–176
Davis M, Walker DL, Miles L, Grillon C (2010) Phasic vs sustained fear in rats and humans: role of the extended amygdala in fear vs anxiety. Neuropsychopharmacology 35(1):105–135
File SE, Hyde JR (1978) Can social interaction be used to measure anxiety? Br J Pharmacol 62(1):19–24
Franklin KBJ, Paxinos G (1997) The mouse brain in stereotaxic coordinates. Academic Press, San Diego, London
Gil OD, Zhang L, Chen S, Ren YQ, Pimenta A, Zanazzi G, Hillman D, Levitt P, Salzer JL (2002) Complementary expression and heterophilic interactions between IgLON family members neurotrimin and LAMP. J Neurobiol 51(3):190–204
Gutman DA, Magnuson M, Majeed W, Keifer OP Jr, Davis M, Ressler KJ, Keilholz S (2013) Mapping of the mouse olfactory system with manganese-enhanced magnetic resonance imaging and diffusion tensor imaging. Brain Struct Funct 218(2):527–537
Hashimoto T, Maekawa S, Miyata S (2009) IgLON cell adhesion molecules regulate synaptogenesis in hippocampal neurons. Cell Biochem Funct 27(7):496–498
Heimer L, Van Hoesen GW (2006) The limbic lobe and its output channels: implications for emotional functions and adaptive behavior. Neurosci Biobehav Rev 30(2):126–147
Horton HL, Levitt P (1988) A unique membrane protein is expressed on early developing limbic system axons and cortical targets. J Neurosci 8(12):4653–4661
Hundahl CA, Allen GC, Hannibal J, Kjaer K, Rehfeld JF, Dewilde S, Nyengaard JR, Kelsen J, Hay-Schmidt A (2010) Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain. Brain Res 1331:58–73
Hyde TM, Bachus SE, Levitt P, Herman MM, Pimenta AF, Kleinman JE, Weinberger DR (1997) Reduction in hippocampal limbic system-associated membrane protein (LAMP) mRNA in schizophrenia. Soc Neurosci Abstr 23:2200
Innos J, Philips MA, Leidmaa E, Heinla I, Raud S, Reemann P, Plaas M, Nurk K, Kurrikoff K, Matto V, Visnapuu T, Mardi P, Kõks S, Vasar E (2011) Lower anxiety and a decrease in agonistic behaviour in Lsamp-deficient mice. Behav Brain Res 217(1):21–31
Innos J, Philips MA, Raud S, Lilleväli K, Kõks S, Vasar E (2012) Deletion of the Lsamp gene lowers sensitivity to stressful environmental manipulations in mice. Behav Brain Res 228(1):74–81
Innos J, Leidmaa E, Philips MA, Sütt S, Alttoa A, Harro J, Kõks S, Vasar E (2013) Lsamp−/− mice display lower sensitivity to amphetamine and have elevated 5-HT turnover. Biochem Biophys Res Commun 430(1):413–418
Koido K, Traks T, Balõtšev R, Eller T, Must A, Koks S, Maron E, Tõru I, Shlik J, Vasar V, Vasar E (2012) Associations between LSAMP gene polymorphisms and major depressive disorder and panic disorder. Transl Psychiatry 2:e152
Koks S, Luuk H, Nelovkov A, Areda T, Vasar E (2004) A screen for genes induced in the amygdaloid area during cat odor exposure. Genes Brain Behav 3(2):80–89
Kötter R, Stephan KE (1997) Useless or helpful? The “limbic system” concept. Rev Neurosci 8(2):139–145
Lam YW, Sherman SM (2010) Functional organization of the somatosensory cortical layer 6 feedback to the thalamus. Cereb Cortex 20(1):13–24
Lamprecht R, Dracheva S, Assoun S, LeDoux JE (2009) Fear conditioning induces distinct patterns of gene expression in lateral amygdala. Genes Brain Behav 8(8):735–743
Lein ES, Hawrylycz MJ, Ao N, Ayres M, Bensinger A, Bernard A, Boe AF, Boguski MS, Brockway KS, Byrnes EJ et al (2007) Genome-wide atlas of gene expression in the adult mouse brain. Nature 445(7124):168–176
Levitt P (1984) A monoclonal antibody to limbic system neurons. Science 223(4633):299–301
Liao CC, Lee LJ (2012) Evidence for structural and functional changes of subplate neurons in developing rat barrel cortex. Brain Struct Funct 217(2):275–292
Luuk H, Koks S, Plaas M, Hannibal J, Rehfeld JF, Vasar E (2008) Distribution of Wfs1 protein in the central nervous system of the mouse and its relation to clinical symptoms of the Wolfram syndrome. J Comp Neurol 509(6):642–660
Mann F, Zhukareva V, Pimenta A, Levitt P, Bolz J (1998) Membrane associated molecules guide limbic and non-limbic thalamocortical projections. J Neurosci 18(22):9409–9419
Marchand A, Faugère A, Coutureau E, Wolff M (2013) A role for anterior thalamic nuclei in contextual fear memory. Brain Struct Funct. [Epub ahead of print]
Morgane PJ, Galler JR, Mokler DJ (2005) A review of systems and networks of the limbic forebrain/limbic midbrain. Prog Neurobiol 75(2):143–160
Must A, Tasa G, Lang A, Vasar E, Kõks S, Maron E, Väli M (2008) Association of limbic system-associated membrane protein (LSAMP) to male completed suicide. BMC Med Genet 23(9):34
Nelovkov A, Areda T, Innos J, Kõks S, Vasar E (2006) Rats displaying distinct exploratory activity also have different expression patterns of gamma-aminobutyric acid- and cholecystokinin-related genes in brain regions. Brain Res 1100(1):21–31
Nieh EH, Kim SY, Namburi P, Tye KM (2012) Optogenetic dissection of neural circuits underlying emotional valence and motivated behaviors. Brain Res 1511:73–92
Philips MA, Vikeså J, Luuk H, Jønson L, Lilleväli K, Rehfeld JF, Vasar E, Kõks S, Nielsen FC (2009) Characterization of MYG1 gene and protein: subcellular distribution and function. Biol Cell 101(6):361–373
Philips MA, Abramov U, Lilleväli K, Luuk H, Kurrikoff K, Raud S, Plaas M, Innos J, Puussaar T, Kõks S, Vasar E (2010) Myg1-deficient mice display alterations in stress-induced responses and reduction of sex-dependent behavioural differences. Behav Brain Res 207(1):182–195
Pimenta AF, Levitt P (2004) Characterization of the genomic structure of the mouse limbic system-associated membrane protein (Lsamp) gene. Genomics 83(5):790–801
Pimenta AF, Reinoso BS, Levitt P (1996) Expression of the mRNAs encoding the limbic system-associated membrane protein (LAMP): II. Fetal rat brain. J Comp Neurol 375(2):289–302
Prensa L, Richard S, Parent A (2003) Chemical anatomy of the human ventral striatum and adjacent basal forebrain structures. J Comp Neurol 460(3):345–367
Raud S, Sütt S, Luuk H, Plaas M, Innos J, Kõks S, Vasar E (2009) Relation between increased anxiety and reduced expression of alpha1 and alpha2 subunits of GABA(A) receptors in Wfs1-deficient mice. Neurosci Lett 460(2):138–142
Reinoso BS, Pimenta AF, Levitt P (1996) Expression of the mRNAs encoding the limbic system-associated membrane protein (LAMP): II. Adult rat brain. J Comp Neurol 375(2):274–288
Tham WW, Stevenson RJ, Miller LA (2009) The functional role of the medio-dorsal thalamic nucleus in olfaction. Brain Res Rev 62(1):109–126
Uroz V, Prensa L, Giménez-Amaya JM (2004) Chemical anatomy of the human paraventricular thalamic nucleus. Synapse 51(3):173–185
Vance (2007) Lsamp gene associated with cardiovascular disease. US Patent 2007/0148661 A1 Pub, 28 Jun 2007
Vogt BA, Gabriel M (1993) Neurobiology of cingulate cortex and limbic thalamus: a comprehensive reference. Birkhäuser, Boston
Yamamoto K, Reiner A (2005) Distribution of the limbic system-associated membrane protein (LAMP) in pigeon forebrain and midbrain. J Comp Neurol 486(3):221–242
Zacco A, Cooper V, Chantler PD, Fisher-Hyland S, Horton HL, Levitt P (1990) Isolation, biochemical characterization and ultrastructural analysis of the limbic system associated membrane protein (LAMP), a protein expressed by neurons comprising functional neural circuits. J Neurosci 10(1):73–90
Acknowledgments
This study was supported by a personal investigation grant from the Estonian Research Council PUT129 (M.A. Philips) and an institutional investigation grant from the Estonian Research Council IUT20-41 (E.Vasar); grants from the Estonian Science Foundation 8408 (K. Lilleväli) and 9099 (H.Luuk), a grant from the Estonian Research Council PUT120 (C.A. Hundahl), a grant from the Ministry of Science and Education (SF0180125s08) and the European Regional Development Fund. We thank professor Luis Puelles from University of Mucia, Spain, and Professor Charles Watson from Curtin University, Australia, for their great insights and help with interpreting anatomical data.
Conflict of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
About this article
Cite this article
Philips, MA., Lilleväli, K., Heinla, I. et al. Lsamp is implicated in the regulation of emotional and social behavior by use of alternative promoters in the brain. Brain Struct Funct 220, 1381–1393 (2015). https://doi.org/10.1007/s00429-014-0732-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00429-014-0732-x