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Features of the clear zone of odontoclasts in the Chinook salmon (Oncorhynchus tshawytscha)

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Abstract

This study aims to clarify the features of the clear zone of odontoclasts on shedding teeth of a teleost fish, Chinook salmon, Oncorhynchus tshawytscha (Walbaum), using a light microscope to determine the orientation between a cell body and a resorptive lacuna, followed by transmission electron microscopy. Ultrathin sections of LR White embedded material were incubated in rabbit anti-actin polyclonal antibody and then were incubated with 15 nm gold-conjugated goat anti-rabbit IgG. The clear zones of odontoclasts showed a variable structure with electron-dense structures on sections, but distinct clear zones were not always seen on odontoclasts. In odontoclasts sectioned in the direction perpendicularly to the surface of a resorptive lacuna, some cells showed a wide clear zone, but two types of clear zones were usually observed: a part composed of some cytoplasmic processes and one composed of several complicatedly interwoven processes. Gold particles were localized on the clear zones, especially in electron-dense structures; very few gold particles were detected in ruffled borders. These results show that the clear zone of odontoclasts in Chinook salmon contains actin. Our results suggest that the clear zone of an odontoclast in Chinook salmon is not always a wide annular structure.

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Abbreviations

CZ:

Clear zone

D:

Dentine

Nu:

Nucleus

RB:

Ruffled border

V:

Vacuole

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Acknowledgements

We wish to thank Mr. N. Satoh and Ms. N. Aruga, Sapporo Salmon Museum (Sapporo, Japan), for providing Chinook salmon. We are also grateful to Mr. Y. Honma and Dr. Y. Nodasaka, Department of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, for their technical assistance; and to C.M.T. van de Sande-Rijkers, Laboratory of Molecular Biology, Leiden University Medical Centre, The Netherlands, for stimulating suggestions to this study. This study was supported by grants from the Japanese Ministry of Education, Science, Sports, Culture and Technology (Grant numbers: 19390548, 15659432, 16591819), and by a grant from the Ministry of Education, Science, Sports, Culture and Technology to promote multidisciplinary research projects (2003).

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Authors and Affiliations

  1. Division of Developmental Biology of Hard Tissue, Department of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Kita 13, Nishi 7, Kita-Ku, 060-8586, Sapporo, Japan

    Takanori Domon, Yumi Taniguchi, Ami Fukui, Shigeru Takahashi, Tsuneyuki Yamamoto & Minoru Wakita

  2. Department of Oral Anatomy II, Asahi University School of Dentistry, Hozumi 1851, Motosu-gun, 501-0296, Gifu, Japan

    Reiko Suzuki

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  1. Takanori Domon
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  2. Yumi Taniguchi
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  3. Ami Fukui
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  4. Reiko Suzuki
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  5. Shigeru Takahashi
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  6. Tsuneyuki Yamamoto
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  7. Minoru Wakita
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Correspondence to Takanori Domon.

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Domon, T., Taniguchi, Y., Fukui, A. et al. Features of the clear zone of odontoclasts in the Chinook salmon (Oncorhynchus tshawytscha). Anat Embryol 211, 87–93 (2006). https://doi.org/10.1007/s00429-005-0061-1

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  • DOI: https://doi.org/10.1007/s00429-005-0061-1

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