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Intraganglionic laminar endings in the rat esophagus contain purinergic P2X2 and P2X3 receptor immunoreactivity

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Abstract

Intraganglionic laminar endings (IGLEs) represent the most prominent vagal afferent terminal structures throughout the gastrointestinal tract. They are most prominent in the esophagus and stomach, but can be found down to the distal colon. Their role as mechanosensors as proposed on anatomical grounds was recently substantiated in elegant functional experiments. There is evidence that vagal mechanosensors in the esophagus and stomach respond to ATP. Thus, the present study aimed at detecting purinergic receptors on IGLEs. IGLEs in the rat esophagus were identified by immunohistochemistry for calretinin and sections were co-incubated with antibodies directed against P2X2 or P2X3 receptors. Also, double label immunocytochemistry for purinergic receptors and calcitonin gene-related peptide as a marker for spinal afferents was performed. Terminal nerve fibers immunoreactive for P2X2 and P2X3, respectively, were observed between outer and inner layers of the tunica muscularis, covering myenteric ganglia totally or partly. Both P2X2 and P2X3 receptor immunoreactivities were highly co-localized with calretinin positive IGLEs as shown by confocal laser scanning microscopy. Numerous calcitonin gene-related peptide immunostained fibers were found to closely approach and intermingle with P2X immunopositive IGLEs. However, there was never co-staining for either of the purinergic receptors and calcitonin gene-related peptide within the same fibers. P2X3 but not P2X2 immunoreactivity was also observed within nerve fiber arborizations in the mucosa of the pharynx. In the nodose ganglion, 8.9±1.1% of P2X2 and 7.2±1.3% of P2X3 immunopositive neurons, respectively, co-stained for calretinin. On the other hand, 63.4±4.6% and 60.1±5.3% of calretinin positive cell bodies contained P2X2 and P2X3 receptor immunoreactivity, respectively. These results indicate that IGLEs are equipped with both P2X2 and P2X3 receptors. Thus, they may act as chemosensors or their mechanosensory properties may be modulated by ATP. It is also suggested that spinal afferents innervating the esophagus are equipped with neither P2X2 nor P2X3 purinergic receptors.

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Acknowledgements

The authors thank Karin Löschner for expert technical assistance, Dr. Marion Raab and Dr. Falk Schrödl for help with the illustrations. This study was supported by Deutsche Forschungsgemeinschaft SFB353/B15.

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  1. Anatomisches Institut, Universität Erlangen-Nürnberg, Krankenhausstrasse 9, 91054, Erlangen, Germany

    Z.-J. Wang & W. L. Neuhuber

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  1. Z.-J. Wang
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  2. W. L. Neuhuber
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Correspondence to W. L. Neuhuber.

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Wang, ZJ., Neuhuber, W.L. Intraganglionic laminar endings in the rat esophagus contain purinergic P2X2 and P2X3 receptor immunoreactivity. Anat Embryol 207, 363–371 (2003). https://doi.org/10.1007/s00429-003-0351-4

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