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Study of the reactive dendritic cells in small B-cell lymphoproliferations of the skin

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Abstract

Distinguishing between low-grade primary cutaneous B-cell lymphoma (LG-pCBCL) and cutaneous lymphoid hyperplasia (CLH) based on histological features is often difficult. CLH lesions contain numerous reactive cells of the histiocyte lineage [Langerhans cells (LC), dermal dendritic cells (DDC), and macrophages], which are also often present in CBCL. The aim of this study was to determine whether immunohistochemical detection of those cells could help differentiate between CLH and LG-pCBCL. We determined the percentages of those histiocytic cells in the dermal infiltrates of 45 cases of cutaneous lymphoproliferations comprising 16 CLH and 29 LG-pCBCL (19 follicle-center cell lymphomas and 10 marginal zone lymphomas) by immunohistochemical labeling with antibodies to CD1a, FXIIIa, and CD68 to respectively detect LC, DDC, and macrophages. To avoid observer-dependent bias, an automated morphometric analysis method was used to recognize immunoreactive cells and calculate their percentages within the infiltrate. FXIIIa+ cells were significatively more frequent in CLH than in LG-pCBCL, whereas CD1a+ and CD68+ cell frequencies were comparable in the two groups. The results of our study suggest that DDC might play an important role in the genesis of cutaneous lymphomas.

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Abbreviations

CLH:

cutaneous lymphoid hyperplasia

CTCL:

cutaneous T cell lymphoma

DC:

dendritic cell

DDC:

dermal dendritic cell

FCL:

follicle-center cell lymphoma

LC:

Langerhans cell

LG-pCBCL:

low-grade primary cutaneous B cell lymphoma

MF:

mycosis fungoides

MZL:

marginal zone lymphoma

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Acknowledgments

We thank Olivier Hongtuanha and Charles Homsi (IMSTAR company) for technical help.

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Authors and Affiliations

  1. Département de Pathologie, Hôpital Henri Mondor, VI AP-HP, Université Paris XII, Créteil, France

    Amélie Carbonnelle, Nadine Martin-Garcia, Nicolas Ortonne & Janine Wechsler

  2. Service de Dermatologie, Hôpital Avicenne, Bobigny, France

    Liliane Laroche

  3. Service de Dermatologie, Hôpital Henri Mondor, VI AP-HP, Université Paris XII, Créteil, France

    Martine Bagot

  4. Service d’Immunologie Biologique, Hôpital Henri Mondor, VI AP-HP, Université Paris XII, Créteil, France

    Valérie Molinier-Frenkel

  5. Department of Pathology, Hôpital Henri Mondor, 51, avenue du Maréchal de Lattre de Tassigny, 94010, Créteil cedex, France

    Janine Wechsler

Authors
  1. Amélie Carbonnelle
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  2. Nadine Martin-Garcia
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  3. Nicolas Ortonne
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  4. Liliane Laroche
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  5. Martine Bagot
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  6. Valérie Molinier-Frenkel
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  7. Janine Wechsler
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Correspondence to Janine Wechsler.

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Carbonnelle, A., Martin-Garcia, N., Ortonne, N. et al. Study of the reactive dendritic cells in small B-cell lymphoproliferations of the skin. Virchows Arch 450, 441–447 (2007). https://doi.org/10.1007/s00428-007-0372-0

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  • DOI: https://doi.org/10.1007/s00428-007-0372-0

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