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Expression of ICAM-1 in colon epithelial cells: an ultrastructural study performed on in vivo and in vitro models

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Abstract

Background

Studies have suggested that in ulcerative colitis (UC), intercellular adhesion molecule-1 (ICAM-1) is involved in migration of leukocytes toward the colonic epithelium. A suitable in vitro model of chronic colonic inflammation does not exist, and the role of the epithelium is based on monolayers of cancer cells. Conflicting results exist on epithelial ICAM-1 expression, and the aim of this study was to compare the expression in various models of colonic epithelium.

Materials and methods

Colonic biopsies from four UC patients and four controls were examined by cryoimmuno-electron microscopy using ICAM-1-antibodies. In four other controls, the epithelium was isolated from colonic biopsies, embedded in collagen, and evaluated similarly. Isolated crypts and cultured cancer cells were stimulated with interferon-γ (IFN-γ) or tumor necrosis factor-α (TNF-α).

Results

ICAM-1 was not expressed in the biopsies. In contrast, HT29 cells and the collagen-embedded crypts expressed ICAM-1 on the apical membranes proximal to the junctional complexes when stimulated with IFN-γ or TNF-α in a dose-related manner.

Conclusions

ICAM-1 is not expressed on colonic epithelium in vivo. However, both colonocytes and HT29 cells were capable of expressing ICAM-1 on their apical membranes in response to supraphysiologic cytokine concentrations. These observations question the justification of extrapolating observations from colon cancer cell lines to in vivo inflammatory conditions.

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Acknowledgements

The authors wish to thank Ms. Hanne Kruse for invaluable technical assistance as well as Jakob Hendel, MD, and Anders Perner, MD, PhD, for assistance in obtaining biopsies. Mesalazine was kindly donated by Søren Halskov, Ferring Pharmaceuticals, Copenhagen, Denmark. The study was supported by a grant from Else and Mogens Wedell-Wedellsborg’s Foundation, Denmark.

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Authors and Affiliations

  1. Department of Pathology, Herlev Hospital, University of Copenhagen, Denmark

    Ben Vainer, Susanne Sørensen & Thomas Horn

  2. Department of Gastroenterology C, Herlev Hospital, University of Copenhagen, Denmark

    Jakob Seidelin & Ole Haagen Nielsen

  3. Department of Pathology, Rigshospitalet, Blegdamsvej 9, 2100 , Copenhagen Ø, Denmark

    Ben Vainer

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  1. Ben Vainer
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  2. Susanne Sørensen
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Correspondence to Ben Vainer.

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Vainer, B., Sørensen, S., Seidelin, J. et al. Expression of ICAM-1 in colon epithelial cells: an ultrastructural study performed on in vivo and in vitro models. Virchows Arch 443, 774–781 (2003). https://doi.org/10.1007/s00428-003-0900-5

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  • DOI: https://doi.org/10.1007/s00428-003-0900-5

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