Abstract
Main conclusion
A stable genetic transformation system for Erigeron breviscapus was developed. We cloned the EbYUC2 gene and genetically transformed it into Arabidopsis thaliana and E. breviscapus. The leaf number, YUC2 gene expression, and the endogenous auxin content in transgenic plants were significantly increased.
Abstract
Erigeron breviscapus is a prescription drug for the clinical treatment of cardiovascular and cerebrovascular diseases. The rosette leaves have the highest content of the major active compound scutellarin and are an important component in the yield of E. breviscapus. However, little is known about the genes related to the leaf number and flowering time of E. breviscapus. In our previous study, we identified three candidate genes related to the leaf number and flowering of E. breviscapus by combining resequencing data and genome-wide association study (GWAS). However, their specific functions remain to be characterized. In this study, we cloned and transformed the previously identified full-length EbYUC2 gene into Arabidopsis thaliana, developed the first stable genetic transformation system for E. breviscapus, and obtained the transgenic plants overexpressing EbYUC2. Compared with wild-type plants, the transgenic plants showed a significant increase in the number of leaves, which was correlated with the increased expression of EbYUC2. Consistently, the endogenous auxin content, particularly indole-3-acetic acid, in transgenic plants was also significantly increased. These results suggest that EbYUC2 may control the leaf number by regulating auxin biosynthesis, thereby laying a foundation for revealing the molecular mechanism governing the leaf number and flowering time of E. breviscapus.
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Data availability
The sequence of EbYUC2 was derived from the E. breviscapus genome deposited at our database (http://medicinalplants.ynau.edu.cn/).
Abbreviations
- IPA:
-
Indole-3-propionic acid
- IAA:
-
Indole-3-acetic acid
- TAA:
-
Tryptophan aminotransferase
- YUC2:
-
Indole-3-pyruvate monooxygenase YUCCA2
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (81960689).
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HSM, SBC, and YSC designed the experiment. ZQ, XJL, and YYH performed the experiments. LYC and ZGH analyzed the data. ZQ, HSM, and YJL prepared the manuscript.
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Zhu, Q., Lu, YC., Xiong, JL. et al. Development of a stable genetic transformation system in Erigeron breviscapus: a case study with EbYUC2 in relation to leaf number and flowering time. Planta 259, 98 (2024). https://doi.org/10.1007/s00425-024-04351-z
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DOI: https://doi.org/10.1007/s00425-024-04351-z