Abstract
Main conclusion
Overexpression and loss of function of OsGEX3 reduce seed setting rates and affect pollen fertility in rice. OsGEX3 positively regulates osmotic stress response by regulating ROS scavenging.
Abstract
GEX3 proteins are conserved in plants. AtGEX3 encodes a plasma membrane protein that plays a crucial role in pollen tube guidance. However, the function of its homolog in rice, OsGEX3, has not been determined. Our results demonstrate that OsGEX3 is localized in the plasma membrane and the nucleus as shown by a transiently transformed assay using Nicotiana benthamiana leaves. The up-regulation of OsGEX3 was detected in response to treatments with polyethylene glycol (PEG) 4000, hydrogen peroxide, and abscisic acid (ABA) via RT-qPCR analysis. Interestingly, we observed a significant decline in the seed setting rates of OsGEX3-OE lines and mutants, compared to the wild type. Further investigations reveal that overexpression and loss of function of OsGEX3 affect pollen maturation. TEM observation revealed a significant decrease in the fertile pollen rates of OsGEX3-OE transgenic lines and Osgex3 mutants due to a delay in pollen development at the late vacuolated stage. Overexpression of OsGEX3 improved osmotic stress and oxidative stress tolerance by enhancing reactive oxygen species (ROS) scavenging in rice seedlings, whereas Osgex3 mutants exhibited an opposite phenotype in osmotic stress. These findings highlight the multifunctional roles of OsGEX3 in pollen development and the response to abiotic stress. The functional characterization of OsGEX3 provides a fundamental basis for rice molecular breeding and can facilitate efforts to cultivate drought resistance and yield-related varieties.
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Abbreviations
- GEX3:
-
Gamete-expressed 3 proteins
- GUS:
-
β-Glucuronidase
- MDA:
-
Malondialdehyde
- OsGEX3:
-
Oryza sativa Gamete-expressed 3 protein
- PCD:
-
Programmed cell death
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- TEM:
-
Transmission electron microscopy
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Acknowledgements
We acknowledge grants from the Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province (U1812401), and the Major Science and Technology Special Project of Fujian Province (2020NZ08016). Han Bao thank Yanbing Li, Dr. Hai Zhang, and Dr. Yong Cui and for suggestions and technical supports of subcellular localization.
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Bao, H., Cui, Y., Ge, L. et al. OsGEX3 affects anther development and improves osmotic stress tolerance in rice. Planta 259, 68 (2024). https://doi.org/10.1007/s00425-024-04342-0
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DOI: https://doi.org/10.1007/s00425-024-04342-0