Abstract
Main conclusion
Plant growth regulators, sucrose concentration, and light quality significantly impact in vitro regeneration of 'Harmony'. Blue light promotes photomorphogenesis by enhancing light energy utilization, adjusting transcription of light signal genes, and altering hormone levels.
Abstract
Hydrangea quercifolia cv. 'Harmony', celebrated for lush green foliage and clusters of white flowers, has been extensively researched for its regenerative properties. Regeneration in stem segments, leaves, and petioles is facilitated by exogenous auxin and cytokinins (CTKs), with the concentration of sucrose (SC) being a key determinant for shoot regeneration from leaves. The study also highlights the significant impact of light conditions on photomorphogenesis. With an increase in the proportion of red (R) light, there is an inhibitory effect, leading to a reduction in leaf area, a decrease in the quantum yield of PSII (ΦPSII), and an increase in non-photochemical quenching (ΦNPQ) and non-regulated energy dissipation in PSII (ΦNO). Conversely, blue (B) light enhances growth, characterized by an increase in leaf area, elevated ΦPSII, and stable ΦNPQ and ΦNO levels. Additionally, B light induces the upregulation of HqCRYs, HqHY5-like, HqXTH27-like, and HqPHYs genes, along with an increase in endogenous CTKs levels, which positively influence photomorphogenesis independent of HqHY5-like regulation. This light condition also suppresses the synthesis of endogenous gibberellins (GA) and brassinosteroids (BR), further facilitating photomorphogenesis. In essence, B light is fundamental in expediting photomorphogenesis in 'Harmony', demonstrating the vital role in plant growth and development.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors are very grateful to Engineer Jin Li for the useful comments on the data analysis of the study, as well as for critically reviewing the manuscript.
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The study was financially supported by the National Natural Science Foundation of China (32201521), the Youth Innovation Promotion Association CAS (Grant No. 2021447), the Key Research and Development Program of Anhui Province (202204c06020045), the Chinese Academy of Sciences-Henan Province Achievement transfer and Transformation Project (2022110 and 2022211), and the Grant of the President Foundation of Hefei Institutes of Physical Science of Chinese Academy of Sciences (YZJJZX202013).
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Su, P., Wang, D., Wang, P. et al. In vitro regeneration, photomorphogenesis and light signaling gene expression in Hydrangea quercifolia cv. 'Harmony' under different LED environments. Planta 259, 71 (2024). https://doi.org/10.1007/s00425-024-04335-z
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DOI: https://doi.org/10.1007/s00425-024-04335-z