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A GARP transcription factor SlGCC positively regulates lateral root development in tomato via auxin-ethylene interplay

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A Correction to this article was published on 09 March 2024

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Abstract

Main conclusion

SlGCC, a GARP transcription factor, functions as a root-related transcriptional repressor. SlGCC synchronizes auxin and ethylene signaling involving SlPIN3 and SlIAA3 as intermediate targets sketching a molecular map for lateral root development in tomato.

Abstract

The root system is crucial for growth and development of plants as it performs basic functions such as providing mechanical support, nutrients and water uptake, pathogen resistance and responds to various stresses. SlGCC, a GARP family transcription factor (TF), exhibited predominant expression in age-dependent (initial to mature stages) tomato root. SlGCC is a transcriptional repressor and is regulated at a transcriptional and translational level by auxin and ethylene. Auxin and ethylene mediated SlGCC protein stability is governed via proteasome degradation pathway during lateral root (LR) growth development. SlGCC over-expressor (OE) and under-expressed (UE) tomato transgenic lines demonstrate its role in LR development. This study is an attempt to unravel the vital role of SlGCC in regulating tomato LR architecture.

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Data availability

All data generated and/ or analyzed in this study are included in the manuscript and its supplementary information.

Change history

Abbreviations

ACC:

1-Aminocyclopropane-1-carboxylic acid

CDS:

Coding sequence

DAG:

Days after germination

GCC:

GARP and coiled-coil

LR:

Lateral root

OE:

Over-expressor

PR:

Primary root

RSA:

Root System Architecture

TF:

Transcription factor

UE:

Under-expressed

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Acknowledgements

We would like to extend gratitude to Prof. Shucai Wang (Northeast Normal University, Changchun, China) for vectors. The authors are thankful to Dr. Mondher Bouzayen (Toulouse, France) for sharing the tomato (Ailsa craig cultivar) seeds. Mr. Ram Awadh for handling and taking care of the transgenics in glasshouse. Funding by CSIR, Delhi (BSC204 and MLP007) is acknowledged. This manuscript represents the CSIR-NBRI communication number CSIR-NBRI_MS/2023/09/01.

Funding

VK and SBT received financial support for this study from CSIR (GOI), AM from DST-INSPIRE(GOI), PP from CSIR funded project MLP007, VAS from CSIR funded BSC204 and MLP007 project.

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  1. Vinod Kumar

    Present address: CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, India

Authors and Affiliations

  1. Molecular Biology and Biotechnology, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Vinod Kumar, Adity Majee, Pooja Patwal, Babythoihoi Sairem, Aniruddha P. Sane & Vidhu A. Sane

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Vinod Kumar, Adity Majee, Pooja Patwal, Babythoihoi Sairem, Aniruddha P. Sane & Vidhu A. Sane

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VK experiments, data analysis, manuscript writing; AM data analysis and manuscript writing; PP and BS experiments and discussion; APS conceptualized and discussion; VAS experiment designing, data analysis, discussion, manuscript writing and fund acquisition.

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Correspondence to Vidhu A. Sane.

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Kumar, V., Majee, A., Patwal, P. et al. A GARP transcription factor SlGCC positively regulates lateral root development in tomato via auxin-ethylene interplay. Planta 259, 55 (2024). https://doi.org/10.1007/s00425-023-04325-7

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