Abstract
Main conclusion
SA and H2O2, in single and mixed elicitation stimulate specialized metabolism and activate oxidative stress in C. tenuiflora plants.
Abstract
Single elicitation with salicylic acid (SA at 75 µM) and, hydrogen peroxide (at 150 µM), and mixed elicitation (75 µM SA + 150 µM H2O2) were evaluated on specialized metabolism in Castilleja tenuiflora Benth. plants. Total phenolic content (TPC), phenylalanine ammonia-lyase (PAL) activity, antioxidant enzymes and specialized metabolite profiles, as well as the expression levels of eight genes involved in phenolic (Cte-TyrDC, Cte-GOT2, Cte-ADD, Cte-AO3, Cte-PAL1, Cte-CHS1) and terpene pathways (Cte-DXS1 and Cte-G10H) and their correlation with major metabolite (verbascoside and aucubin) concentrations were investigated. TPC content (three-fold) and PAL activity (11.5-fold) increased with mixed elicitation, as well as catalase and peroxidase activity (11.3-fold and 10.8-fold, respectively), compared to single elicitation. Phenylethanoid accumulation was greatest under mixed elicitation, followed by SA and H2O2. Lignan accumulation was differential, depending on the plant part and the elicitor. Flavonoids only appeared after mixed elicitation. The high concentration of verbascoside under mixed elicitation was related to a high gene expression. Single elicitation induced iridoid accumulation in specific parts (H2O2 in aerial parts and SA in roots), whereas under mixed elicitation, it accumulated in both parts. A high concentration of aucubin in the aerial part was related to a high expression level of genes of the terpene pathway Cte-DXS1 and Cte-G10H, and in the root with Cte-G10H, while Cte-DXS1 was downregulated in this tissue in all treatments. Mixed elicitation with SA and H2O2 represents an interesting tool to increase the production of specialized metabolites in plants.
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All data supporting the findings of the present study are available within the paper and its supplementary information files. Requests for material for scientific purposes should be made to the corresponding author.
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Abbreviations
- AO3:
-
Primary amine oxidase
- ADD:
-
Aryl alcohol dehydrogenase
- CAT:
-
Catalase
- CHS:
-
Chalcone synthase
- DM:
-
Dry matter
- DXS:
-
1-Deoxy-d-xylulose-5-phosphate synthase
- G10H:
-
Geraniol 10-hydroxylase
- GOT2:
-
Aspartate aminotransferase mitochondrial
- PAL:
-
Phenylalanine ammonia-lyase
- POD:
-
Peroxidase
- SA:
-
Salicylic acid
- TPC:
-
Total phenolic compounds
- TyrDC:
-
Tyrosine decarboxylase
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The authors acknowledge Instituto Politécnico Nacional for the financial support.
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This work was financed by the Instituto Politécnico Nacional (Grants SIP 2020-20200523; 2183-20220492). ERR received a doctoral fellowship from the Consejo Nacional de Ciencia y Tecnología (Grant 706094) and the BEIFI-IPN Program (SIP 2020-20200522). The funding sources had no role in study design, collection, analysis, or interpretation of data, or in the writing of the manuscript and the decision to submit the manuscript.
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Rubio-Rodríguez, E., Vera-Reyes, I., Rodríguez-Hernández, A.A. et al. Mixed elicitation with salicylic acid and hydrogen peroxide modulates the phenolic and iridoid pathways in Castilleja tenuiflora plants. Planta 258, 20 (2023). https://doi.org/10.1007/s00425-023-04177-1
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DOI: https://doi.org/10.1007/s00425-023-04177-1