Abstract
Main conclusion
We characterized an efficient chimeric sub-genomic transcript promoter from Horseradish Latent Virus, FHS4, active in both dicot and monocot plants, and it could be a potential tool for plant biotechnology.
Abstract
Plant pararetroviruses are a rich source of novel plant promoters widely used for biotechnological applications. Here, we comprehensively characterized a unique sub-genomic transcript (Sgt) promoter of Horseradish Latent Virus (HRLV) and identified a fragment (HS4; − 340 to + 10; 351 bp) that showed the highest expression of reporter genes in both transient and transgenic assays as evidenced by biochemical, histochemical GUS reporter assay and transcript analysis of uidA gene by qRT-PCR. Phylogenetic analysis showed that the HSgt promoter was closely related to the sub-genomic promoter of the Cauliflower Mosaic Virus (CaMV19S). We found that the as-1 element and W-box played an important role in the transcriptional activity of the HS4 promoter. Furthermore, the HS4 promoter was also induced by salicylic acid. Alongside, we enhanced the activity of the HS4 promoter by coupling the enhancer region from Figwort Mosaic Virus (FMV) promoter to the upstream region of it. This hybrid promoter FHS4 was around 1.1 times stronger than the most commonly used promoter, 35S (Cauliflower Mosaic Virus full-length transcript promoter), and was efficient in driving reporter genes in both dicot and monocot plants. Subsequently, transgenic tobacco plants expressing an anti-microbial peptide BrLTP2.1 (Brassica rapa lipid transport protein 2.1), under the control of the FHS4 promoter, were developed. The in vitro anti-fungal assay revealed that the plant-derived BrLTP2.1 protein driven by an FHS4 promoter manifested increased resistance against an important plant fungal pathogen, Alternaria alternata. Finally, we concluded that the FHS4 promoter can be used as an alternative to the 35S promoter and has a high potential to become an efficient tool in plant biotechnology.
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Abbreviations
- HRLV:
-
Horseradish Latent Virus
- HS4:
-
Horseradish Latent Virus sub-genomic transcript promoter fragment 4 (− 340 to + 10)
- Flt:
-
Full-length transcript
- Sgt:
-
Sub-genomic transcript
- CRE:
-
Cis-regulatory element
- CaMV:
-
Cauliflower mosaic virus
- FMV:
-
Figwort mosaic virus
- MMV:
-
Mirabilis mosaic virus
- DaMV:
-
Dahlia mosaic virus
- UAS:
-
Upstream activation sequence
- FUAS:
-
Figwort mosaic virus upstream activation sequence
- CP:
-
Core promoter
- GUS:
-
β-Glucuronidase
- GFP:
-
Green fluorescent protein
- AMP:
-
Anti-microbial peptide
- BrLTP2.1:
-
Brassica rapa Lipid transport protein 2.1
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Acknowledgements
The study was supported by funds from Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India, under Grant No. CRG/2021/001001 and Council of Scientific and Industrial Research (CSIR), Government of India, under grant number 09/657(0076)/2020-EMR-I.
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Sherpa, T., Jha, D.K., Kumari, K. et al. Synthetic sub-genomic transcript promoter from Horseradish Latent Virus (HRLV). Planta 257, 40 (2023). https://doi.org/10.1007/s00425-023-04066-7
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DOI: https://doi.org/10.1007/s00425-023-04066-7