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AtFTCD-L, a trans-Golgi network localized protein, modulates root growth of Arabidopsis in high-concentration agar culture medium

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Abstract

Main conclusion

AtFTCD-L protein is localized on the TGN vesicles in Arabidopsis root cap cells. AtFTCD-L mutation resulted in slow root growth of Arabidopsis in high-concentration agar culture medium.

Abstract

Arabidopsis formiminotransferase cyclodeaminase-like protein (AtFTCD-L) in Arabidopsis is homologous to the formiminotransferase cyclodeaminase (FTCD) protein in animal cells. However, the localization and function of AtFTCD-L remain unknown in Arabidopsis. In this study, we generated and analyzed a deletion mutant of AtFTCD-L with a T-DNA insertion. We found that the growth of Arabidopsis roots with the T-DNA insertion mutation in AtFTCD-L was slower than that of wild-type roots when grown in high-concentration 1/2 MS agar culture medium. AtFTCD-L-GFP could restore the ftcd-l mutant phenotype. In addition, the AtFTCD-L protein was localized on the trans-Golgi network (TGN) vesicles in Arabidopsis root cap cells. Fluorescence recovery after photobleaching (FRAP) experiment using Arabidopsis pollen-specific receptor-like kinase-GFP (AtPRK1-GFP) stably transformed plants showed that the deficiency of AtFTCD-L protein in Arabidopsis led to slower secretion in the root cap peripheral cells. The AtFTCD-L protein deficiency also resulted in a significantly reduced monosaccharides content in the culture medium. Based on the above results, we speculate that the AtFTCD-L protein may be involved in sorting and/or transportation of TGN vesicles in root cap peripheral cells, thereby regulating the extracellular secretion of mucilage components in the root cap.

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All data generated or analyzed during this study are included in the manuscript.

Abbreviations

AtFTCD-L:

Arabidopsis Formiminotransferase cyclodeaminase-like protein

AtPRK1:

Arabidopsis Pollen-specific receptor-like kinase 1

FRAP:

Fluorescence recovery after photobleaching

TGN:

trans-Golgi network

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Acknowledgements

We thank the National Institute for Agricultural Resesrch (INRA) for providing the T-DNA insertion line. We thank Dr. Zhenbiao Yang (University of California, Riverside) for the gift of the plasmid contained AtPRK1. We thank Dr. Yan Guo (China Agricultural University) for kindly providing the seeds of the Arabidopsis line expressing mCherry-VTI12. This study was supported by the National Natural Science Foundation of China (30971435 and 31270230).

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  1. Qijiang Cao and Wei Zhang have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Qijiang Cao, Wei Zhang, Xinyan Liu & Yan Li

  2. College of Life Sciences and Engineering, Shenyang University, Liaoning, 110044, China

    Qijiang Cao

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  1. Qijiang Cao
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Cao, Q., Zhang, W., Liu, X. et al. AtFTCD-L, a trans-Golgi network localized protein, modulates root growth of Arabidopsis in high-concentration agar culture medium. Planta 256, 3 (2022). https://doi.org/10.1007/s00425-022-03911-5

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