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Genome-wide transcriptome analysis and characterization of the cytochrome P450 flavonoid biosynthesis genes in pigeon pea (Cajanus cajan)

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Abstract

Main conclusion

226 CcCYP450 genes were identified at the genomic level and were classified into 45 clades based on phylogenetic analysis. CcCYP75B165 gene was found that might play important roles in the biosynthesis of flavonoids in pigeon pea, and was significantly induced by methyl jasmonate (MeJA).

Abstract

The cytochrome P450 mono-oxygenase (CYP450) superfamily plays a key role in the flavonoid biosynthesis pathway and resists different kinds of stresses. Several CYP450 genes have been identified to be involved in the biosynthesis of crop protection agents. However, the CcCYP450 genes from pigeon pea have not been identified. Here, 226 CcCYP450 genes were identified at the genomic level by analysing the gene structure, distribution on chromosomes, gene duplication, and conserved motifs and were classified into 45 clades based on phylogenetic analysis. RNA-seq analysis revealed clear details of CcCYP450 genes that varied with time of MeJA (methyl jasmonate) induction. Among them, six CcCYP450 subfamily genes were found that might play important roles in the biosynthesis of flavonoids in pigeon pea. The overexpression of CcCYP75B165 in pigeon pea significantly induced the accumulation of genistin and downregulated the contents of cajaninstilbene acid, apigenin, isovitexin, and genistein and the expression of flavonoid synthase genes. This study provides theoretical guidance and plant genetic resources for cultivating new pigeon pea varieties with high flavonoid contents and exploring the molecular mechanisms of the biosynthesis of flavonoids under MeJA treatment.

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Data availability

The authors declare that all data supporting the findings of this study are available within the article and its supplementary information files.

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Acknowledgements

This work was supported by the State Key Program of National Natural Science Foundation of China (31930076), the Fundamental Research Funds for the Central Universities (2572020AW03), the 111 Project (B20088), and Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team).

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  1. Jie Yang and Hongquan Li have contributed equally to this work.

Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China

    Jie Yang, Hongquan Li, Ruijin Ma, Yuanhang Chang, Xiangyu Qin & Jian Xu

  2. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China

    Jie Yang, Hongquan Li, Ruijin Ma, Yuanhang Chang, Xiangyu Qin & Jian Xu

  3. College of Forestry, Beijing Forestry University, Beijing, 100083, China

    Yujie Fu

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  1. Jie Yang
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Yang, J., Li, H., Ma, R. et al. Genome-wide transcriptome analysis and characterization of the cytochrome P450 flavonoid biosynthesis genes in pigeon pea (Cajanus cajan). Planta 255, 120 (2022). https://doi.org/10.1007/s00425-022-03896-1

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