Abstract
Main conclusion
Soybean contains a group of 64 L-type lectin receptor-like kinases. Three LecRKs were involved in the interactions with Phytophthora sojae and Bradyrhizobium diazoefficiens.
Abstract
L-type lectin receptor-like kinases (LecRKs) comprise an important class of membrane-localized receptor-like kinases that are involved in plant adaptation. In this study, we performed an inventory analysis of LecRKs in Glycine max (soybean). In total, 64 GmLecRKs containing the canonical LecRK feature were identified. Phylogenetic analysis revealed that 48 GmLecRKs have close orthologs in Arabidopsis or Solanum lycopersicum, while 16 are likely present only in the leguminous plant species. Transcriptome analyses revealed that expressions of multiple GmLecRK genes are either induced or suppressed during infection by the soybean root rot pathogen Phytophthora sojae. In addition, overexpression of the three LecRKs (Glyma.17G085000, Glyma.05G041300 or Glyma.17G224600) in the soybean hairy roots enhanced resistance to P. sojae. Upon inoculation with Bradyrhizobium diazoefficiens, overexpression of Glyma.17G085000 in the soybean hairy roots does not significantly influence the nodulation, while overexpression of Glyma.05G041300 or Glyma.17G224600 slightly reduced the number and dry weight of nodules. This study highlights the importance of LecRKs in regulating plant–microbe interactions and provides new knowledge on the deployment of LecRKs to increase resistance in soybean.
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Data availability
Data analyzed during this study are included in this published article and its supplementary information files.
Abbreviations
- dai:
-
Day after inoculation
- LecRKs:
-
L-type lectin receptor-like kinases
- SD:
-
Segmental duplication
- TD:
-
Tandem duplication
- WGD:
-
Whole genome duplication
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Acknowledgements
We thank Dr. Yao Zhao, Dr. Mian Wu, Zhichao Zhang and Maozhu Yin for suggestions. This project was supported by China Natural Science Foundation grants 31501622 and 31872927, and by “the Fundamental Research Funds for the Central Universities KJQN201663”.
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This article was funded by National Natural Science Foundation of China (31501622, and 31872927); Fundamental Research Funds for the Central Universities (KJQN201663).
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Zeng, M., Wan, B., Wang, L. et al. Identification and characterization of L-type lectin receptor-like kinases involved in Glycine max–Phytophthora sojae interaction. Planta 254, 128 (2021). https://doi.org/10.1007/s00425-021-03789-9
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DOI: https://doi.org/10.1007/s00425-021-03789-9